Objective To analyze the correlation between the expression of ubiquitin binding enzyme variant 1A(UEV1A), ubiquitin ligase E2N(UBE2N) and the expression of epithelial mesenchymal transformation(EMT) gene in breast cancer and its clinical prognostic significance.Methods One hundred and twenty-four breast cancer patients diagnosed and treated by Qionghai People's Hospital/Hainan Eastern Regional Medical Center from February 2016 to February 2017 were selected as the study subjects. The expressions of UEV1A, UBE2N and EMT pathway genes E-cadherin(E-cad), N-cadherin(N-cad), vim and Snail mRNA in breast cancer tissues and adjacent tissues were detected by real-time fluorescent quantitative PCR; Immunohistochemical method was used to detect the levels of UEV1A and UBE2N proteins in tissues. To compare the difference of UEV1A and UBE2N protein levels in breast cancer patients with different clinicopathological characteristics. Pearson correlation analysis and Spearman rank correlation analysis were used to analyze the correlation between UEV1A, UBE2N mRNA and EMT related gene expression in breast cancer tissues, as well as the correlation between UEV1A and UBE2N protein levels in cancer tissues. Kaplan Meier survival analysis: The impact of UEV1A and UBE2N protein levels on the survival and prognosis of breast cancer patients. Multivariate Cox regression analysis of factors affecting the survival and prognosis of breast cancer patients.Results UEV1A, UBE2N, N-cad, Vim, and Snail mRNA expression in breast cancer tissues were higher than those in adjacent noncancerous tissues, while E-cad mRNA expression was lower than that in adjacent noncancerous tissues(t/P=28.803/<0.001, 48.654/<0.001, 54.411/<0.001, 46.213/<0.001, 27.273/<0.001, 22.228/<0.001). UEV1A and UBE2N mRNA expression were positively correlated with N-cad, Vim, and Snail mRNA expression in cancer tissues(UEV1A:r=0.694, 0.702, 0.806,P<0.001 for all; UBE2N:r=0.717, 0.756, 0.803,P<0.001 for all), and negatively correlated with E-cad mRNA expression(r=-0.741,-0.756,P<0.001 for both). The positive expression rate of UEV1A and UBE2N proteins in breast cancer tissues was significantly higher than that in adjacent noncancerous tissues(χ2/P=68.896/<0.001, 64.851/<0.001); there was a significant positive correlation between UEV1A and UBE2N protein expression in cancer tissues(r/P=0.785/<0.001). The positive expression rate of UEV1A and UBE2N proteins in breast cancer tissues was higher in patients with T2-4 stage and lymph node metastasis than in those with T1 stage and without lymph node metastasis(UEV1A:χ2/P=29.803/<0.001, 4.015/0.045; UBE2N: χ2/P=7.038/0.008, 8.572/0.003). Breast cancer patients with positive UEV1A expression had a lower 5-year overall survival rate than those with negative expression(χ2/P=5.085/0.024), and those with positive UBE2N expression had a lower 5-year overall survival rate than those with negative expression(χ2/P=8.391/0.004). T2-4 stage, lymph node metastasis, positive UEV1A expression, and positive UBE2N expression were independent risk factors affecting the survival prognosis of breast cancer patients [OR(95%CI)=1.777(1.299-2.432), 1.613(1.193-2.181), 1.838(1.302-2.596), 1.735(1.224-2.459),P<0.001 for all].Conclusion The expression of UEV1A and UBE2N in breast cancer tissue is increased, which are related to tumor stage and lymph node metastasis, and are independent risk factors affecting the prognosis of breast cancer patients.

Ｏｂｊｅｃｔｉｖｅ To investigate the expression of β⁃catenin and long chain non coding RNA (lncRNA) KC⁃
NQ1OT1 in serum of patients with chronic hepatitis B and their application value in the diagnosis of liver fibrosis. Ｍｅｔｈｏｄｓ
A total of 90 patients with chronic hepatitis B admitted to the First Affiliated Hospital of Air Force Military Medical Uni⁃
versity from January 2020 to January 2021 were selected. According to the degree of liver fibrosis, they were divided into
23 patients without liver fibrosis (S0 group), 42 patients with mild liver fibrosis (S1 － S2 group), and 25 patients with mod⁃
erate to severe liver fibrosis (S3 － S4 group); In addition, 30 patients undergoing physical examination in the same hospital
were selected as the healthy control group. Compare the serum expression levels of β⁃catenin and lncRNA KCNQ1OT1
and indicators of liver fibrosis; analysis the correlation between the expression levels of β⁃catenin and lncRNA KC⁃
NQ1OT1 and the degree of liver fibrosis and its indicators; Logistic regression analysis was used to analyze the influen⁃
cing factors of liver fibrosis in patients with chronic hepatitis B; receiver operation characteristic curve (ROC) analysis the
predictive value of serum β⁃catenin and lncRNA KCNQ1OT1 levels in the development of liver fibrosis in patients withchronic hepatitis B. Ｒｅｓｕｌｔｓ Serum β⁃catenin and lncRNA KCNQ1OT1 levels inhealthy control group, S0 group, S1⁃S2group, S3⁃S4 group increased sequentially (F/P = 28.066/ < 0.001, 25.173/ < 0.001). The levels of hyaluronic acid (HA), pro⁃
collagen N⁃terminal peptide (P III P), type IV collagen (C IV), and laminin (LN) were gradually increased (F/P = 79.414/ <
0.001, 119.630/ < 0.001, 104.574/ < 0.001, 96.631/ < 0.001). The levels of serum β⁃catenin and lncRNA KCNQ1OT1 were sig⁃nificantly and positively correlated with the degree of liver fibrosis (
r/P = 0.598/0.001, 0.643/0.009). β⁃Catenin and lncRNA
KCNQ1OT1 levels were significantly positively correlated with liver fibrosis indicators HA, P III P, C IV, and LN（ β⁃cate⁃nin：
r/P = 0.483/0.016, 0.456/0.013, 0.641/0.006, 0.519/0.008； Serum lncRNA KCNQ1OT1:r/P = 0.496/0.014, 0.604/0.007, 0.523/
0.014, 0.611/0.002). Elevated levels of serum β⁃catenin, lncRNA, KCNQ1OT1, HA, P III P, C IV, and LN are risk factors forliver fibrosis in patients with chronic hepatitis B ［
OR(95% CI) = 1.564 (1.205 － 2.030), 2.213 (1.449 － 3.379), 1.816 (1.261 －2.615), 2.315 (1.380 － 3.884), 1.564 (1.161 － 2.107), and 3.013 (1.491 － 6.090)］ . The AUC of serum β⁃catenin, lncRNA, KC⁃NQ1OT1, and their combination in predicting liver fibrosis in patients with chronic hepatitis B were 0.784, 0.775, and 0.857,respectively. The combination of the two was superior to their respective prediction alone (Z/P = 2.617/0.028, 2.897/0.014).Ｃｏｎｃｌｕｓｉｏｎ The levels of serum β⁃catenin and lncRNA KCNQ1OT1 from patients with chronic hepatitis B are closelyrelated to the degree of liver fibrosis, and the combined detection of the two has a good reference value for the occur⁃rence of liver fibrosis in patients with chronic hepatitis B.

Ｏｂｊｅｃｔｉｖｅ To observe the effect of ketogenic diet (KD) combined with high intensity interval training
(HIIT) on nonalcoholic fatty liver (NAFL). Ｍｅｔｈｏｄｓ From June 2020 to March 2022, 112 patients with NAFL admitted to
the Infectious Disease/Liver Disease Center of the First Affiliated Hospital of Xinjiang Medical University were selected as
the study subjects. They were divided into the control group and the observation group with a ratio of 1:1 according to the
random number table, with 56 patients in each group. The control group was treated with HIIT intervention, and the obser⁃
vation group was treated with KD intervention on the basis of the control group. Obesity indexes (body mass index (BMI),
waist to hip ratio (WHR), obesity degree, body fat rate (Fat)), glucose metabolism indexes ［ fasting insulin (FINS), fasting
blood glucose (FPG), insulin resistance index (HOMA⁃IR)］ , lipid metabolism indexes (TG), low density lipoprotein cholesterol
(LDL⁃C), high density lipoprotein cholesterol (HDL⁃C) Inflammatory factors ［ high sensitivity C⁃reactive protein (hs⁃CRP), in⁃
terleukin⁃6 (IL⁃6), IL⁃8］ , sterol regulatory element binding protein⁃1 (SREBP⁃1c), retinol binding protein⁃4 (RBP4), muscle
mass , bone mass level. Ｒｅｓｕｌｔｓ After 3 months of intervention, BMI, WHR, obesity and Fat in the observation group werelower than those in the control group (
t/P = 6.183/ < 0.001, 4.224/ < 0.001, 3.362/0.001, 3.268/0.001), FINS, FPG, HOMA⁃IR lev⁃els were lower than those in the control group (
t/P = 2.888/0.005, 2.983/0.004, 2.459/0.016), TG, LDL⁃C levels were lower thanthose in the control group, HDL⁃C levels were higher than those in the control group (
t/P = 3.582/ < 0.001, 3.416/ < 0.001,4.036/ < 0.001), The level of serum hs⁃CRP, IL⁃6 ,IL⁃8 was lower than that of the control group (t/P = 3.109/0.002, 3.862/ <0.001, 3.431/ < 0.001), and the level of SREBP⁃1c and RBP4 in serum was lower than that of the control group (t/P = 5.248/ <0.001, 3.978/0.002). The muscle mass was higher than that of the control group (t/P = 2.613/0.010). There was no significantdifference in bone mass between the two groups before and after the intervention (P > 0.05). Ｃｏｎｃｌｕｓｉｏｎ The interventioneffect of KD and HIIT on NAFL is better, which can effectively control obesity, reduce body mass, and regulate glycolipidmetabolism, inflammatory factors and serum SREBP⁃1c, RBP4 levels, without affecting muscle mass and bone mass.

Ｏｂｊｅｃｔｉｖｅ To analyze the changes and clinical significance of plasma miR⁃15b and vascular endothelial
growth factor (VEGF) levels in premature infants with bronchopulmonary dysplasia. Ｍｅｔｈｏｄｓ A total of 148 premature in⁃
fants admitted to the Department of Neonatology, Beijing Children's Hospital, Capital Medical University, from March 2020
to March 2022 were collected. They were divided into bronchopulmonary dysplasia group (65 cases ) and normal develop⁃
ment group (normal group, 83 cases) based on whether or not they had bronchopulmonary dysplasia. Real⁃time fluorescence
quantitative polymerase chain reaction was used to detect the expression of miR⁃15b in plasma, and enzyme linked immu⁃
nosorbent assay was used to detect the level of VEGF in plasma. Relevant clinical data were collected. Pearson correlation
coefficient describes the correlation between miR⁃15b and VEGF, and multivariate logistic regression analysis is used to ana⁃
lyze the factors affecting bronchopulmonary dysplasia in premature infants. The value of miR⁃15b and VEGF in the auxiliary
diagnosis of bronchopulmonary dysplasia in preterm infants was analyzed by subject performance characteristic curve (ROC)
analysis. Ｒｅｓｕｌｔｓ The expression of plasma miR⁃15b in the adverse group was higher than that in the normal group, and
the level of VEGF was lower than that in the normal group (
t/P = 12.754/ < 0.001, 31.023/ < 0.001). The expression of plasma
miR⁃15b was negatively correlated with VEGF (
r/P = － 0.617/ < 0.001). Large gestational age, high partial pressure of oxygen,
and high VEGF are protective factors for bronchopulmonary dysplasia in premature infants, while high miR⁃15b is a risk fac⁃
tor ［
OR(95% CI) = 0.899 (0.845 － 0.958), 0.521 (0.356 － 0.764), 0.600 (0.442 － 0.814), and 1.540 (1.220 － 1.945)］ . The area under
the curve of miR⁃15b, VEGF, and their combination in the diagnosis of bronchopulmonary dysplasia in premature infants was
0.728, 0.756, and 0.931, respectively, and the combination of the two was higher than that of the single diagnosis (
Z/P/ < 0.001, 4.207/ < 0.001). Ｃｏｎｃｌｕｓｉｏｎ The expression of miR⁃15b in the plasma of premature infants with bronchopul⁃
monary dysplasia is upregulated, and the level of VEGF is decreased. miR⁃15b may negatively regulate VEGF participation in
the process of bronchopulmonary dysplasia.

Ｏｂｊｅｃｔｉｖｅ To investigate the ratio of peripheral blood helper T cells 17 (Th17 cells ) to regulatory Tcells (Treg cells) and the level of urinary monocyte chemoattractant protein⁃1 (MCP⁃1) in patients with systemic lupus ery⁃thematosus (SLE). Ｍｅｔｈｏｄｓ A total of 68 patients with SLE admitted to Shiyan People's Hospital from July 2018 to Sep⁃
tember 2020 were selected and divided into a non lupus nephritis group of 28 patients (non⁃LN group) and a lupus nephritisgroup of 40 patients (LN group) based on whether or not the SLE patients had renal involvement. During the same period,35 healthy physical examinees from the hospital were included in the healthy control group. Collect clinical data of subjects,
use flow cytometry to detect the ratio of Th17 cells to Treg cells in peripheral blood mononuclear cells (PBMC), and use RT⁃PCR technology to detect the corresponding transcription factor retinoic acid related solitary nuclear receptor (ROR⁃ γ t) ofTh17 cells and Treg cells)， and the expression of forked helical wing like transcription factor 3 (Foxp3) mRNA. Urine MCP1 levels were measured using ELISA. Spearman correlation was used to analyze the relationship between Th17/Treg cell ratioand urine MCP⁃1, as well as its correlation with clinical indicators. Subjects' performance characteristic curve (ROC) was usedto analyze Th17/Treg cell ratio and urine MCP⁃1 levels in the diagnosis of LN in patients with SLE. Ｒｅｓｕｌｔｓ The ratio ofTh17 cells in LN group and non LN group was higher than that in healthy control group (F = 12.345,P = 0.015). The ratio ofTreg cells in LN group < non⁃LN group < healthy control group (F = 22.388,P < 0.001), and the ratio of Th17/Treg cells in LNgroup>non⁃LNgroup > healthy control group (F = 26.674,P < 0.001); Comparison of ROR⁃ γ t mRNA expression levels inLN group > non⁃LN group > healthy control group (F = 20.578,
P < 0.001). The expression level of Foxp3 mRNA in LN groupand non⁃LN group was lower than that in healthy control group (F = 7.782,P = 0.003); Comparison of urinary MCP⁃1 levels inLN group > non⁃LN group > healthy control group (F = 51.231,P < 0.001). Spearman correlation analysis showed that therewas a positive correlation between the Th17/Treg cell ratio and the urine MCP⁃1 level in SLE patients (r = 0.604,P = 0.017), apositive correlation between the Th17/Treg cell ratio and the SLEDAI score in SLE patients (
r = 0.534,P = 0.021), and a posi⁃tive correlation between the urine MCP⁃1 level and the SLEDAI score and 24⁃hour urine protein content (
r/P = 0.342/ < 0.001,0.412/0.023). The ROC curve showed that the area under curve (AUC) of Th17/Treg cell ratio and urine MCP⁃1 in predicting
LN in SLE patients was 0.718 and 0.769, with sensitivity of 0.782 and 0.993, and specificity of 0.736 and 0.569.Ｃｏｎｃｌｕｓｉｏｎ
In patients with SLE and LN, there is an imbalance in the ratio of Th17/Treg cells and an increase in the level of MCP⁃1 in
urine. The combined detection of the two can be used as an indicator of disease activity in SLE patients, and is helpful in
assessing the progress of LN.