Objective To investigate the changes and clinical significance of serum macrophage inflammatory protein-1α(MIP-1α) and interleukin-35(IL-35) levels in patients with autoimmune thyroiditis. Methods From April 2021 to May 2024, 103 patients with autoimmune thyroiditis who visited our hospital were regarded as the observation group, and 106 healthy volunteers who underwent physical checkups at our hospital were regarded as the control group. ELISA method was applied to detect the levels of serum IL-1, IL-6, IL-17, TNFα, MIP-1α, IL-35, thyroid peroxidase antibody, and thyroid globulin antibody. Pearson method was applied to analyze the correlation between serum MIP-1α and IL-35 levels in autoimmune thyroiditis. Logistic regression was applied to analyze the related factors of autoimmune thyroiditis. ROC curve was applied to analyze the efficacy of serum MIP-1α and IL-35 levels in diagnosing autoimmune thyroiditis. Results The serum levels of MIP-1α(t/P=9.911/<0.001) in the observation group were higher than those in the healthy control group, and the serum IL-35(t/P=9.102/<0.001) levels lower than those of healthy controls; Pearson analysis showed that serum MIP-1α was negatively correlated with IL-35 in patients with autoimmune thyroiditis(r=-0.621,P<0.001),MIP-1α was positively correlated with TPOAb, TGAb, T3, FT3, FSH, T4and FSH, and negatively correlated with FT4. IL-35 was negatively correlated with TPOAb, TGAb, T3, FT3, FSH, T4and FSH, and positively correlated with FT4(r=0.531, 0.517, 0.594, 0.562, 0.504, 0.601,-0.475,-0.519,-0.524,-0.496,-0.575,-0.524,-0.617, 0.498,P<0.001). Elevated MIP-1α levels were an independent risk factor for autoimmune thyroiditis[OR(95%CI)=2.157(1.208-3.853)], and elevated IL-35 levels were an independent protective factor for autoimmune thyroiditis[OR(95%CI)=0.617(0.502-0.758)]. The serum MIP-1α and IL-35 levels and the area under the curve(AUC) of the combined diagnosis of autoimmune thyroiditis were 0.849, 0.862 and 0.950, respectively, and the combined diagnosis of autoimmune thyroiditis was better than the diagnosis of serum MIP-1α and IL-35 levels alone(Z/P=3.390/<0.001, 3.039/0.002). Conclusion Serum MIP-1α level in patients with autoimmune thyroiditis is prominently higher than that in the healthy population, while IL-35 level is prominently lower than that in the healthy population. Changes in their levels can effectively diagnose autoimmune thyroiditis.

Objective To detect the expression of serum kisspeptin and forkhead box O subtype 3(FOXO3) in patients with polycystic ovary syndrome(POS), and analyze the prognostic value of kisspeptin and FOXO3 expression in polycystic ovary syndrome. Methods From March 2021 to March 2024, 94 patients with polycystic ovary syndrome who visited to the Gynecology Department of Yan'an People's Hospital were selected as the study group. According to the prognosis of the patients after 3 months of treatment, they were assigned into a poor prognosis subgroup(38 cases) and a good prognosis subgroup(56 cases). Additionally, 60 healthy women of childbearing age who underwent physical checkup at our hospital were included as the healthy control group. ELISA method was applied to detect serum kisspeptin. RT-qPCR method was applied to detect serum FOXO3. Multivariate logistic regression was applied to analyze the influencing factors of poor prognosis in patients. Receiver operating characteristic(ROC) curve analysis of serum kisspeptin and FOXO3 for predicting poor prognosis in patients with polycystic ovary syndrome. Results The serum level of kisspeptin in the study group was higher than that in the healthy control group, and the level of FOXO3 was lower than that in the healthy control group(t/P=9.388/<0.001, 10.087/<0.001). The levels of luteinizing hormone and kisspeptin in the poor prognosis subgroup were higher than those in the good prognosis subgroup, and FOXO3 was lower than that in the good prognosis subgroup(t/P=4.774/<0.001, 7.147/<0.001, 8.269/<0.001). Elevated levels of luteinizing hormone and kisspeptin were independent risk factors for poor prognosis in patients with polycystic ovary syndrome[OR(95%CI)=2.455(1.402-4.300), 2.174(1.432-3.300)], while elevated level of FOXO3 was a independent protective factor[OR(95%CI)=0.142(0.067-0.301)]. The AUC of predicting poor prognosis in patients with polycystic ovary syndrome based on serum levels of kisspeptin and FOXO3, as well as their combined AUC, were 0.791, 0.792, and 0.906, respectively. The AUC of combined was greater than the AUC predicted by serum levels of kisspeptin and FOXO3 alone(Z/P=2.060/0.039, 2.544/0.011). Conclusion Patients with polycystic ovary syndrome have higher serum level of kisspeptin and lower level of FOXO3. Elevated levels of serum kispeptin and decreased levels of FOXO3 are factors affecting poor prognosis in patients. The combined detection of serum kisspeptin and FOXO3 has certain significance for the prognosis of patients with polycystic ovary syndrome.

Objective To explore the application value of antinuclear antibody(ANA) and antinuclear antibody spectrum(ANAs) in the diagnosis of systemic lupus erythematosus(cSLE) in children. Methods A total of 96 children diagnosed with SLE in the Department of Nephrology and Immunology of our hospital were selected as the experimental group, 86 children with other rheumatic diseases were selected as the disease control group, and 60 healthy subjects were selected as the healthy control group. The expression of ANA and ANAs in serum was detected by indirect immunofluorescence and immunoblotting, respectively. The positive expression was analyzed and the diagnostic efficacy of different diagnostic methods was compared. The results of fluorescence karyotype and specific antibody spectrum were analyzed statistically. Results The expression of ANA in cSLE(90.6%) was significantly higher than that in disease control group(36.0%) and healthy control group(3.3%, P<0.05). The fluorescent karyotypes of cSLE children mainly included nuclear granular type in 21 cases(24.1%), nuclear homogeneous type in 25 cases(28.7%) and related mixed type in 41 cases(47.2%). The positive rates of nRNP/Sm, Sm, SSA, Ro-52, dsDNA, anti-nucleosome, anti-histone and anti-RIB in ANAs of cSLE children were significantly different from those of the disease control group and the healthy control group(P<0.01). The dsDNA antibodies, anti-nucleosome antibodies and anti-histone antibodies were dominant in nuclear homogeneity type and related mixed type, anti-NRNP/Sm antibodies, SSA antibodies and Ro52 antibodies were dominant in nuclear particle type and related mixed type, anti-RIB antibodies were dominant in cytoplasmic particle type and related mixed type. There was general consistency between ANA and ANAs(Kappa=0.443, P<0.05), and the sensitivity of the combined detection increased. Conclusion ANA and ANAs have their respective advantages and are correlated with each other. There is a certain correlation between ANA and ANAs antibody. The combined detection of ANA and ANAs can greatly improve the sensitivity and reduce the missed diagnosis rate of children with SLE, which is of great significance for the early diagnosis and timely treatment of children with cSLE.

Objective To explore the clinical efficacy, immune function and quality of life of patients with obstructive sleep apnea hypopnea syndrome(OSAHS) after multiplanar low temperature plasma radiofrequency ablation(LTPRA). Methods A total of 120 OSAHS patients admitted to the Otolaryngology Department of Xincheng Branch of Xuzhou Central Hospital from January 2020 to January 2024 were selected as the study objects, and were divided into control group(62 cases) and observation group(58 cases) according to random number table method. The control group was treated with plasma-assisted modified uvulopalatopharyngoplasty(H-UPPP), and the observation group was treated with multiplane(LTPRA). The clinical efficacy, related symptoms, immune function and quality of life before and after treatment were compared between the two groups. Results The total effective rate of observation group was higher than that of control group(98.28% vs. 80.65%,χ2=4.550,P<0.001). Compared with before treatment, sleep apnea hypopnea index(AHI) decreased and minimum blood oxygen saturation(LSAT) increased in OSAHS patients in 2 groups 3 days after surgery, and the decrease and increase were more significant in the observation group(t=4.931,P<0.001;t=6.330,P<0.001). At 3 days after surgery, CD4+T lymphocytes and CD4+/CD8+ decreased and CD8+T lymphocytes increased in patients with OSAHS in the 2 groups, and the increases of CD4+T lymphocytes, CD4+/CD8+ and CD8+T lymphocytes decreased in the observation group were greater than those in the control group(t=6.993;P<0.001;t=2.955,P=0.004;t=9.879,P<0.001). At 6 months after surgery, the scores of sleep apnea Quality of life index(SAQLI) of patients with OSAHS in both groups were higher than those before surgery, and the SAQLI scores in the observation group were higher than those in the control group(t=9.877,P<0.001). Conclusion Multiplanar LTPRA can effectively improve the immunity and quality of life of OSAHS patients, and enhance the clinical efficacy.

Objective To investigate the effects of etomidate(Eto) on the proliferation, apoptosis, and invasion of human gastric cancer cells by regulating the microRNA(miR)-204-5p/homologous box gene C8(HOXC8) axis. Methods Experiments were conducted in the Laboratory of Wuhan University of Science and Technology in April 2023 to April 2024. Gastric cancer cells MKN45 were separated into control(Ctrl) group, low-dose Eto group(Eto-L, 5 μmol/L), medium-dose Eto group(Eto-M, 10 μmol/L), high-dose Eto group(Eto-H, 20 μmol/L), Eto-H+miR inhibitor NC, and Eto-H+miR-204-5p inhibitor group. CCK-8 method and colony formation assay were applied to detect cell proliferation. Flow cytometry was applied to detect apoptosis in MKN45 cells. Transwell assay was applied to detect cell invasion ability. QRT-PCR was applied to detect the mRNA levels of miR-204-5p and HOXC8 in cells. Dual luciferase assay was applied to detect the targeting relationship between miR-204-5p and HOXC8. Results Compared with the control group, the survival rate of MKN45 cells(F/P=33.391/<0.001), colony formation rate(F/P=29.646/<0.001), cell invasion rate(F/P=44.814/<0.001), and HOXC8 mRNA level(F/P=45.485/<0.001) in Eto-L, Eto-M, and Eto-H groups were greatly lower while the apoptosis rate(F/P=78.091/<0.001) and miR-204-5p level(F/P=22.665/<0.001) were greatly higher. Compared with the Eto-H group and Eto-H+miR inhibitor NC group, the survival rate of MKN45 cells(q/P=8.099/<0.001), colony formation rate(q/P=7.857/<0.001), cell invasion rate(q/P=7.768/<0.001), and HOXC8 mRNA level(q/P=11.162/<0.001) in Eto-H+miR-204-5p inhibitor group were greatly higher(P<0.05), while the apoptosis rate(q/P=10.254/<0.001) and miR-204-5p level(q/P=8.596/<0.001) were greatly lower. Compared with co transfected cells with HOXC8-WT and miR-NC, the relative luciferase activity of MKN45 cells co transfected with HOXC8-WT and miR-204-5p mimic was greatly lower(t/P=5.770/<0.001). Conclusion Eto may weaken the proliferation and invasion abilities of gastric cancer cells, and promote cell apoptosis by upregulating miR-204-5p and downregulating HOXC8.