Objective To investigate the expression and prognostic value of serum tenascin C(TNC) and bromodomain PHD-finger transcription factor(BPTF) in patients with pancreatic cancer.Methods A total of 102 patients with pancreatic cancer diagnosed and treated in the Department of Gastroenterology, Heilongjiang Provincial Hospital from January2020 to January 2022 were selected as the pancreatic cancer group, and 60 healthy subjects were selected as the healthy control group. Serum TNC and BPTF levels were measured by enzyme-linked immunosorbent assay. The expression levels of serum TNC and BPTF in pancreatic cancer patients with different clinicopathological parameters were compared. The prognostic value of serum TNC and BPTF levels was evaluated using receiver operating characteristic(ROC) curve analysis. KaplanMeier survival curves and Cox regression were used to analyze the relationship between serum TNC, BPTF levels and prognosis in pancreatic cancer patients.Results Serum TNC and BPTF levels in pancreatic cancer patients were significantly higher than those in healthy controls(t/P = 34.850/<0.001, 26.931/<0.001). Patients with tumor stages ⅡB-Ⅲ and lymph node metastasis showed higher serum TNC and BPTF levels compared to those with stages Ⅰ-ⅡA and no lymph node metastasis(TNC:t/P = 16.041/<0.001, 11.132/<0.001; BPTF: t/P = 16.377/<0.001, 11.416/<0.001). The AUC values of serum TNC, BPTF, and their combination for predicting pancreatic cancer prognosis were 0.862, 0.846, and 0.921, respectively. The combined predictive value was superior to that of either biomarker alone(Z/P = 4.551/<0.001, 4.558/<0.001). The 3-year overall survival rate in the TNC high-expression subgroup was lower than that in the TNC low-expression subgroup(Log-rankχ 2= 16.120,P<0.001),and the 3-year overall survival rate in the BPTF high-expression subgroup was lower than that in the BPTF low-expression subgroup(Log-rank χ 2= 13.380, P < 0.001). Tumor stage Ⅱ B-Ⅲ, lymph node metastasis, high TNC expression, and high BPTF expression were identified as independent risk factors for prognosis in pancreatic cancer patients [OR(95%CI) = 1.774(1.217-2.584), 1.587(1.272-1.981), 1.404(1.140-1.728), 1.507(1.150-1.975)].Conclusion Elevated serum TNC and BPTF levels in pancreatic cancer are associated with tumor stage and lymph node metastasis. The combination of TNC and BPTF demonstrates good predictive value for the prognosis of pancreatic cancer patients.

Objective To analyze the expression levels of SRY box-related gene 7(SOX7) and zinc finger E-box binding protein 1(ZEB1) in pancreatic cancer tissues and their relationship with patient prognosis. Methods A total of 115 patients with pancreatic cancer admitted to the Department of Hepatobiliary Surgery, East Campus, Jinan Central Hospital from January 2021 to April 2022 were included in the study. During surgery, both cancerous and adjacent tissues were resected from the patients, and the expression levels of SOX7 and ZEB1 proteins in the tissues were detected by immunohistochemistry. Patient pathological characteristics were collected. The patients were followed up for 3 years, during which 48 patients survived and 67 patients died. Multivariate Cox regression analysis was performed to identify prognostic factors. The relationship between SOX7 and ZEB1 expression levels and prognosis was analyzed using the Kaplan-Meier method. Results Compared with adjacent tissues, the positive expression rate of SOX7 in cancer tissues decreased, while the positive expression rate of ZEB1 increased(χ 2/P = 28.186/<0.001). Compared with pancreatic cancer patients without lymph node metastasis, with stage I tumors, and invasion depth of Tis-T2, the positive expression rate of SOX7 in patients with lymph node metastasis, stage Ⅲtumors, and invasion depth of T3-4 was decreased(χ 2/P = 4.973, 9.534, 45.083/0.026, 0.009, < 0.001), while the positive expression rate of ZEB1 increased(χ 2/P = 7.547, 40.573, 7.952/0.006, < 0.001, 0.005). The 3-year survival rate of patients with positive SOX7 expression(36/44, 81.82%) was higher than that of patients with negative SOX7 expression(12/71, 16.90%)(χ 2= 47.077,P<0.001). The 3-year survival rate of patients with positive ZEB1 expression(31/95, 32.63%) was lower than that of patients with negative ZEB1 expression(17/20, 85.00%)( χ 2= 18.633, P<0.001). Positive expression of ZEB1 was identified as a risk factor for death in pancreatic cancer patients, while positive expression of SOX7 was a protective factor[HR( 95%CI) =0.409(0.282-0.592), 2.341(1.655-3.312)].Conclusion The positive rate of SOX7 is decreased and that of ZEB1 is increased in pancreatic cancer. Changes in the expression of both are closely related to patient prognosis.

Objective To investigate the clinical efficacy of preoperative short-course radiotherapy(SCRT) combined with anti-programmed death receptor-1(PD-1) monoclonal antibody in elderly patients with locally advanced rectal cancer.Methods A retrospective study was conducted on rectal cancer patients admitted to Minda Hospital Affiliated to Hubei Minzu University from June 2021 to June 2024. After screening, 80 patients were enrolled and divided into an observation group(n = 40) and a control group(n = 40). The observation group received SCRT(25 Gy/5 fractions over 5 days) combined with tirelizumab(200 mg intravenous infusion on day 1, repeated every 3 weeks for 2 cycles) followed by radical surgery. The control group underwent radical surgery 8 to 12 weeks after SCRT alone. Perioperative indicators, disease remission rates, and postoperative complications were compared between the two groups.Results The positive rate of serum SDC2 decreased significantly after surgery in both groups compared to preoperative levels(P<0.05). The observation group showed a significantly greater reduction in SDC2 expression than the control group(χ 2= 5.59,P = 0.018). Postoperative levels of CD3 +, CD4 +, and CD4 +/CD8 +decreased significantly in both groups(all P<0.05), but these levels were significantly higher in the observation group than in the control group(P <0.05). no="" significant="" differences="" were="" observed="" in="" operative="" intraoperative="" blood="" time="" to="" first="" bowel="" duration="" of="" abdominal="" or="" length="" hospital="" all="" p="">0.05).The objective response rate did not differ significantly between the observation and control groups(75.00% vs. 62.50%,χ 2=1.455,P = 0.228), but the disease control rate was significantly higher in the observation group(95.00% vs. 77.50%, χ 2=5.165, P = 0.023). Postoperative complication rates were comparable between the two groups(15.00% vs. 17.50%,χ 2= 0.092,P = 0.762).Conclusion Preoperative SCRT combined with PD-1 monoclonal antibody can effectively improve disease control rates, reduce serum SDC2 positivity, and enhance immune function without compromising surgical safety in elderly patients with locally advanced rectal cancer.

Objective To investigate the expression levels of serum soluble triggering receptor expressed on myeloid cells-1(sTREM-1) and dipeptidyl peptidase Ⅲ(DPP3) in patients with acute coronary syndrome(ACS) complicated with cardiogenic shock(CS) and their relationship with prognosis.Methods A total of 137 patients with ACS complicated with CS admitted to the First Affiliated Hospital of Hunan University of Traditional Chinese Medicine from January 2021 to December 2024 were selected as the ACS-CS group. According to 28-day survival after admission, patients were divided into a survival subgroup(n = 86) and a death subgroup(n = 51). Additionally, 137 patients with uncomplicated ACS during the same period were selected as the ACS group, and 137 healthy subjects were enrolled as the healthy control group. Serum s TREM-1 and DPP3 levels were measured by enzyme-linked immunosorbent assay. Spearman rank correlation was used to analyze the correlation between serum s TREM-1, DPP3 levels and CS severity. Cox proportional hazards regression was used to analyze factors influencing prognosis and death in ACS patients with CS. The receiver operating characteristic(ROC) curve was applied to evaluate the predictive value of serum s TREM-1 and DPP3 levels for the risk of death in ACS patients with CS.Results Serum s TREM-1 and DPP3 levels in the ACS-CS group and ACS group were higher than those in the healthy control group, and levels in the ACS-CS group were higher than those in the ACS group(P<0.05). Serum s TREM-1 and DPP3 levels in the typical phase, deterioration phase, and end-stage phase were higher than those in the risk phase and initial phase, and levels in the deterioration and end-stage phases were higher than those in the typical phase(P <0.05). Serum s TREM-1 and DPP3 were positively correlated with disease severity(r s/P = 0.516/0.001, 0.559/0.001). Compared with the risk phase, the risk of death increased in patients in the typical phase, deterioration phase, and end-stage phase(HR = 1.445, 2.670, 6.633,P<0.05). Elevated LVEF was a protective factor against death(HR = 0.875,P <0.05), while elevated s TREM-1 and elevated DPP3 were independent risk factors(HR = 1.626, 1.714,P <0.05). The AUC values of serum s TREM-1, DPP3, and their combination in predicting the risk of death were 0.866, 0.859, and 0.935, respectively. The combined predictive value was greater than that of either single indicator(Z/P = 2.089/0.036,2.192/0.028).Conclusion Serum s TREM-1 and DPP3 expression is upregulated in patients with ACS complicated with CS and is closely related to CS severity and prognosis. Early combined detection may serve as a potential biomarker for assessing disease status and predicting prognosis.

Objective To analyze the risk factors of acute biliary pancreatitis(ABP) complicated with pancreatic pseudocyst(PPC), and to construct a risk prediction model and verify it. Methods A total of 149 ABP patients admitted to the Department of General Surgery of the First People ' s Hospital of Kashi Prefecture from June 2022 to June 2024 were retrospectively selected as the modeling group. According to the occurrence of PPC, the patients were divided into PPC group and non-PPC group. The baseline data of the two groups were compared, and the risk factors were determined by binary Logistic regression analysis, and the prediction model was constructed. At the same time, 27 patients with ABP admitted from July 2024 to December 2024 were selected as the validation group to verify and analyze the clinical application value of the prediction model. Results Of the 149 patients with ABP, 30(20.13 %) patients with PPC were included in the PPC group, and119(79.87 %) patients without PPC were included in the non-PPC group. The drinking history, diabetes history, seizure frequency, ABP severity and fasting blood glucose level in PPC group were significantly higher than those in non-PPC group(t/χ 2/P = 6.256/0.012, 4.070/0.044, 10.883/<0.001, 10.227/0.001), while the serum albumin level was significantly lower than that in non-PPC group(t/P = 3.347/0.001). Binary Logistic regression analysis showed that drinking history and the number of ABP episodes were independent risk factors for PPC in ABP patients [OR(95%CI) = 10.957(1.740-69.038), 4.127(1.540-11.065)], while high serum albumin was a protective factor for PPC [OR(95%CI) = 0.372(0.176-0.812)].The area under the receiver operating characteristic(ROC) curve of the modeling group was 0.804, and the area under the ROC curve of the validation group was 0.912. The Hosmer-Lemeshow test showed that the performance of the model was close to the ideal predictive performance(χ 2= 14.47,P = 0.071). Conclusion Repeated episodes of ABP and alcohol abuse are independent risk factors for PPC in patients with ABP, while higher serum albumin is a protective factor. The nomogram prediction model based on these factors has good prediction efficiency and can provide a quantitative tool for early clinical identification of high-risk patients.