Objective To explore the value of magnifying staining endoscopy combined with Vascular endothelial growth factor(VEGF), CD34, CD105 and other angiogenesis molecules in the diagnosis of early esophageal cancer.Method One hundred and fifty-two patients with suspected early esophageal cancer or precancerous lesions detected at the Endoscopic Diagnosis and Treatment Center of Hainan Provincial People's Hospital from January 2022 to December 2022 were selected for classification by the Japanese Esophageal Society(JES) under magnifying endoscopy, and their serum levels of VEGF, CD34, and CD105 were measured. Build a column chart model using R software, and evaluate the diagnostic efficacy of the column chart and various serological indicators using the receiver operating characteristic curve(ROC). Results Among 152 suspected early esophageal cancer or precancerous lesions patients, 80 cases were classified as type A(11 cases of early cancer), 51 cases as type B1(36 cases of early cancer), 14 cases as type B2(10 cases of early cancer), and 7 cases as type B3(6 cases of early cancer). The sensitivity and specificity of JES classification using magnifying endoscopy for diagnosing early esophageal cancer were 0.730 and 0.663, respectively; Serum VEGF, CD34, and CD105 gradually increased in patients with inflammatory response, mild atypical hyperplasia, severe atypical hyperplasia, and early esophageal cancer(F/P=1 536.000/<0.001,1 133.000/<0.001,3 156.000/<0.001), and their area under the curve(AUC) for diagnosing early esophageal cancer were 0.821, 0.772, and 0.687, respectively; The AUC of the diagnostic model for JES typing combined with serum biomarker nomograms under magnifying endoscopy is 0.922. Conclusion The combination of magnifying endoscopy JES typing and serum markers such as VEGF, CD34, and CD105 can be used for the diagnosis of early esophageal cancer, and its diagnostic accuracy is significantly higher than a single indicator.

Objective To explore the predictive value of serum carcinoembryonic antigen(CEA), C-reactive protein(CRP) and interleukin-6(IL-6) in response to neoadjuvant radiotherapy and chemotherapy for advanced rectal cancer, and to construct a related nomogram model. Methods From January 2019 to January 2022, 104 patients with advanced rectal cancer diagnosed and treated by liver laparoscopic surgery in the Digestive Vascular surgery Center of the First Affiliated Hospital of Xinjiang Medical University were selected as the research objects. According to different neoadjuvant chemotherapy treatment reactions, they were divided into a good response group of 21 patients and a poor response group of 83 patients. The clinical data, serum tumor markers and inflammatory factor expression levels of the two groups were compared; Multivariate logistic regression analysis is used to investigate the influencing factors of neoadjuvant radiotherapy and chemotherapy response in mid to late-stage rectal cancer patients. A column chart model is constructed to predict neoadjuvant radiotherapy and chemotherapy response in mid to late-stage rectal cancer patients. The calibration curve of the column chart model is plotted, and subject work characteristic curve(ROC) analysis and decision curve analysis are performed to evaluate the calibration ability and predictive effectiveness of the column chart model. Results The proportion of maximum tumor diameter, poorly differentiated tumor differentiation, TNM/T stage 4, and lymph node metastasis in the poorly responsive group was significantly higher than that in the well responsive group[(χ2)/P=3.536/<0.001, 5.785/0.029, 7.086/0.009, 5.349/0.034]; Poor response group serum CRP, IL-6, tumor necrosis factor-α(TNF-α) CEA, carbohydrate antigen 19-9(CA19-9), Vascular endothelial growth factor(VEGF) were significantly higher than those in the well responded group(t/P=5.232/<0.001, 5.352/<0.001, 2.255/0.022, 4.462/<0.001, 2.145/0.031, 3.859/<0.001); the="" results="" of="" multivariate="" logistic="" regression="" analysis="" showed="" that="" high="" and="" il-6="" were="" independent="" risk="" factors="" affecting="" response="" to="" neoadjuvant="" radiotherapy="" chemotherapy="" in="" patients="" with="" advanced="" rectal="" cancer="" .="" a="" column="" chart="" model="" was="" constructed="" analyzed="" using="" calibration="" predicted="" value="" poor="" is="" good="" agreement="" actual="" observed="" p="">0.05). ROC analysis results show that the area under the curve(AUC) predicted by the combination of serum CEA, CRP, and IL-6 is 0.943(95% CI 0.834-0.987). Decision curve analysis results show that within most reasonable threshold probability ranges, The overall net return predicted by the combination of the three indicators is higher than that of a single indicator(Z/P=5.879/<0.001, 2.578/0.006, 2.178/0.023). Conclusion Serum CEA, CRP, and IL-6 are independent predictive factors affecting the response to neoadjuvant radiotherapy and chemotherapy in patients with advanced rectal cancer. The column chart model constructed by combining three indicators has high predictive power and accuracy.

Objective To observe the levels of serum amyloid A4(SAA4) and kallikrein B1(KLKB1) in patients with nonmuscular invasive bladder cancer(NMIBC) and their effects on the prognosis after transurethral resection of bladder tumor(TURBT).Methods One hundred NMIBC patients who received TURBT treatment in the Urology Department of Beijing Ditan Hospital Affiliated with Capital Medical University from January 2017 to December 2019 were selected as the research subjects, and 60 healthy individuals who underwent physical examinations during the same period were selected as the control group. Enzyme linked immunosorbent assay(ELISA) was used to detect serum levels of SAA4 and KLKB1 in NMIBC patients. Compare the serum levels of SAA4 and KLKB1 in NMIBC patients with different clinical and pathological characteristics. Pearson correlation analysis of the correlation between serum, SAA4, and KLKB1 levels in NMIBC patients. Kaplan Meier survival curve analysis(Log Rank test): The impact of serum SAA4 and KLKB1 levels on the prognosis of progression free survival(PFS) in NMIBC patients. COX proportional risk regression model was used to analyze the influencing factors of PFS prognosis in NMIBC patients.Results The serum levels of SAA4 and KLKB1 in the NMIBC group were on average higher than those in the control group(t=31.508, 22.825, P<0.001), and there was a positive correlation between serum SAA4 and KLKB1 levels(r=0.725, P<0.001). The serum levels of SAA4 and KLKB1 in T1 stage and high-grade NMIBC cancer were higher than those in Ta/tis stage and low-grade cancer tissues, respectively(t=7.244, 9.255, 13.718, 16.681, P<0.001). The 3-year PFS of patients with high and low levels of SAA4 were 42.86%(21/49) and 74.51%(38/51), respectively. The 3-year cumulative PFS of patients in the high-level SAA4 group was significantly lower than that of patients in the low-level group(χ2/P=8.275/0.004). The 3-year PFS of the KLKB1 high-level group and low-level group were 41.67%(20/48) and 75.00%(39/52), respectively. The 3-year cumulative PFS of patients in the high-level KLKB1 group was significantly lower than that of patients in the low-level group(χ2/P=10.420/0.001). Tumor stage T1, high pathological grade, high levels of SAA4, and KLKB1 are independent risk factors affecting the prognosis of PFS in NMIBC patients [HR(95%CI)=1.614(1.319-2.799), 1.917(1.319-2.799), 1.839(1.228-2.753), 1.744(1.245-2.443)].Conclusion The elevated levels of serum SAA4 and KLKB1 in NMIBC patients are associated with tumor TNM staging and pathological grading, and are independent factors affecting the prognosis of PFS in NMIBC patients.

Objective To analyze the relationship between the expression of differentially expressed genes(lncRNA CRNDE) and microRNA-29a-3p(miR-29a-3p) in colorectal tumors with long chain non coding RNA in renal cell carcinoma(RCC) tissue, clinical pathological features, and prognosis. Methods From January 2018 to November 2019, 92 patients with RCC were selected from the Urology of the People's Hospital of Xinjiang. qPCR was used to detect the expression of lncRNA CRNDE and miR-29a-3p in RCC tissues and paracancerous tissues, and analyze their correlation and relationship with clinicopathological characteristics. The survival curves of RCC patients with different lncRNA CRNDE and miR-29a-3p expressions were plotted using the K-M method, and the influencing factors of mortality in RCC patients were analyzed using multivariate Cox regression. Results Compared with adjacent tissues, the expression of lncRNA and CRNDE in RCC cancer tissue increased, while the expression of miR-29a-3p decreased(t=16.050, 21.147, P<0.001). Pearson correlation analysis showed a negative correlation between lncRNA CRNDE and miR-29a-3p expression in RCC cancer tissue(r/P=-0.739/<0.001). In RCC cancer tissue, lncRNA CRNDE expression was higher in WHO/ISUP grades 3-4, TNM stages III-IV, and with lymph node metastasis than in WHO/ISUP grades 1-2, TNM stages I-II, and without lymph node metastasis(t/P=3.041/0.003, 3.183/0.002, 3.598/0.001), while miR-29a-3p expression was lower in WHO/ISUP grades 1-2, TNM stages I-II, and without lymph node metastasis(t/P=3.197/0.002, 2.962/0.004, 3.421/0.001). Following up for 3 years, the cumulative survival rate of 92 RCC patients was 81.52%(75/92). K-M survival curve analysis showed that the cumulative survival rate of the lncRNA CRNDE high expression group was lower than that of the lncRNA CRNDE low expression group, and the cumulative survival rate of the miR-29a-3p high expression group was higher than that of the miR-29a-3p low expression group(χ2/P=4.346/0.037, 5.773/0.016). Multivariate Cox regression analysis showed that WHO/ISUP grade 3-4, TNM stage III-IV, lymph node metastasis, and high lncRNA CRNDE were independent risk factors for mortality in RCC patients, while high miR-29a-3p was an independent protective factor [HR(95% CI)=4.112(1.676-10.088), 4.664(1.696-12.825), 5.806(1.881-17.919), 1.552(1.144-2.105), 0.524(0.312-0.883)].Conclusion The high expression of lncRNA CRNDE and low expression of miR-29a-3p in RCC tissue are related to WHO/ISUP grading, TNM staging, lymph node metastasis, and prognosis, and may become new targets for the diagnosis and treatment of RCC.

Objective To explore the correlation between serum Dephosphorylation uncarboxylated matrix Gla protein(dp-ucMGP), osteocalcin(OC) levels and intracranial calcification of internal carotid artery in patients with ischemic stroke. Methods One hundred and seventy-two patients with ischemic stroke who were admitted to the Neurology, the Second Hospital of Baoding City, Hebei Province from December 2020 to October 2022 were selected as the research objects. The vascular wall calcification score was divided into 37 cases of no calcification group, 95 cases of mild calcification group, and 40 cases of severe calcification group. Collect clinical data of patients; Detect serum biochemical indicators and levels of dp-ucMGP and OC in patients; Analyze the correlation between serum levels of dp-ucMGP and OC with age, smoking history, and 25-hydroxyvitamin D [25(OH) D] in patients with ischemic stroke. Logistic regression analysis is used to identify the risk factors for intracranial carotid artery calcification in patients with ischemic stroke. The receiver operating characteristic curve(ROC) is used to analyze the predictive value of serum levels of dp-ucMGP and OC for mild and severe intracranial carotid artery calcification in patients with ischemic stroke. Results Compared with the non calcification group, the mild calcification group and the severe calcification group showed an increase in age, smoking history, and a decrease in 25(OH) D levels(F=5.901,P=0.003,χ2=8.963, P=0.011, F=33.403, P<0.001); Compared with the mild calcification group, the severe calcification group showed a decrease in 25(OH) D levels(P<0.05). The levels of serum dp-ucMGP and OC in the non calcification group, mild calcification group, and severe calcification group increased sequentially(F/P=101.057/<0.001, 11.668/<0.001). The serum dp ucMGP level was significantly positively correlated with OC(r/P=0.563/<0.001). High levels of dp-ucMGP and OC are risk factors for intracranial carotid artery calcification in ischemic stroke patients [OR(95% CI)=2.329(1.510-3.591), 1.735(1.010-2.980)]. The serum levels of dp-ucMGP and OC, as well as their combined prediction of AUC for mild calcification in the intracranial segment of the internal carotid artery in patients with ischemic stroke, were 0.857, 0.825, and 0.913, respectively. The combined AUC of the two was greater than the AUC predicted separately(Z/P=2.869/0.004, 2.861/0.004). The serum levels of dp-ucMGP, OC, and their combined prediction of severe calcification in the intracranial segment of the internal carotid artery in patients with ischemic stroke were 0.876, 0.809, and 0.945, respectively. The combined AUC of the two was greater than the AUC predicted separately(Z/P=2.314/0.021, 3.012/0.003). Conclusion The serum levels of dp-ucMGP and OC in patients with ischemic stroke increase with the degree of calcification in the intracranial segment of the internal carotid artery, which has certain predictive value for the degree of calcification in the intracranial segment of the internal carotid artery.