Objective To analyze the effect of antisense RNA 1(lncRNA ROR1-AS1) targeting microRNA-504(miR-504) on the proliferation, migration and invasion of human hepatoma cell line MHCC97H by long chain non-coding RNA tyrosine protein kinase transmembrane receptor 1.Methods The cancer tissues and adjacent tissues of 32 patients with liver cancer were collected from the Department of Hepatobiliary Surgery of the Affiliated Hospital of Inner Mongolia Medical University from June 2019 to May 2021. MHCC97H cells were divided into si-ROR1-AS1 group, si-NC group, miR-504 mimics group, miR-NC group, si-ROR1-AS1+anti-miR-504 group and si-ROR1-AS1+anti-miR-NC group, and the corresponding plasmids were transfected. The expression of ROR1-AS1 and miR-504 in tissue samples and cells was detected by qRT-PCR. Cell proliferation was detected by clonogenic assay and MTT assay. Transwell detected cell migration and invasion. The levels of cyclin-dependent kinase inhibitor 1A(p21), matrix metalloproteinase-2(MMP-2), MMP-9 and E-cadherin were detected by Western-blot method. The regulatory relationship between ROR1-AS1 and miR-504 was detected by double luciferase report experiment. Results The level of ROR1-AS1 in cancer tissue of patients with liver cancer was higher than that in paracancerous tissue, and the level of miR-504 was lower than that in paracancerous tissue(t=11.544, 10.905, P<0.001). Compared with si-NC group, the number of MHCC97H cell clones, cell survival rate, number of migrating cells, number of invasive cells, and levels of MMP-2 and MMP-9 in si-ROR1-AS1 group decreased(t=8.978, 9.647, 8.444, 13.282, 10.026, 12.006, P<0.001), and the levels of miR-504, p21, E-cadherin increased(t=10.527, 9.722, 12.901, P<0.001). Compared with the miR-NC group, the number of MHCC97H cell clones, cell survival rate, number of migrating cells, number of invasive cells and the level of MMP-2 and MMP-9 in the miR-504 mimics group decreased(t=8.831, 9.680, 8.187, 12.480, 10.026, 10.954, P<0.001), and the level of p21 and E-cadherin protein increased(t=9.418, 12.614, P<0.001). The double luciferase report experiment showed that ROR1-AS1 could target the regulation of miR-504. Compared with si-ROR1-AS1+anti-miR-NC group, si-ROR1-AS1+anti-miR-504 group increased the number of cell clones, cell survival rate, number of migrating cells, number of invasive cells, and levels of MMP-2 and MMP-9(t=7.064, 7.012, 6.746, 10.222, 7.213, 7.982, P<0.001), while p21 and E-cadherin protein levels decreased(t=4.841, 7.120, P<0.001).Conclusion Interference with the expression of ROR1-AS1 can inhibit the proliferation, migration and invasion of hepatoma MHCC97H cells. The specific mechanism may be related to the targeted miR-504 level.

Liver cancer;                    Long non-coding RNA ROR1-AS1;                    microRNA-504;                    Proliferation;                    Migration;                    Invasion;                    Mechanism;