Ｄｅｔｅｃｔｉｏｎ ａｎｄ ｓｉｇｎｉｆｉｃａｎｃｅ ｏｆ ｐｅｒｉｐｈｅｒａｌ ｂｌｏｏｄ Ｔｈ１７ ／ Ｔｒｅｇ ｃｅｌｌｓ ａｎｄ ｕｒｉｎａｒｙ ｍｏｎｏｃｙｔｅ ｃｈｅｍｏａｔｔｒａｃｔａｎｔ ｐｒｏｔｅｉｎ⁃１ ｉｎ
ｐａｔｉｅｎｔｓ ｗｉｔｈ ｓｙｓｔｅｍｉｃ ｌｕｐｕｓ ｅｒｙｔｈｅｍａｔｏｓｕｓ Ｚｈｅｎｇ Ｘｕｅｎａ， Ｙａｎｇ Ｈｕａｑｉａｎｇ， Ｌｉ Ｂｏｊｕｎ， Ｑｉｎ Ｊｕｎ． Ｄｅｐａｒｔｍｅｎｔ ｏｆ Ｈｅｍａｔｏｌｏ⁃
ｇｙ， Ｒｅｎｍｉｎ Ｈｏｓｐｉｔａｌ， Ｈｕｂｅｉ Ｕｎｉｖｅｒｓｉｔｙ ｏｆ Ｍｅｄｉｃｉｎｅ， Ｈｕｂｅｉ Ｐｒｏｖｉｎｃｅ， Ｓｈｉｙａｎ ４４２０００， Ｃｈｉｎａ
Ｃｏｒｒｅｓｐｏｎｄｉｎｇ ａｕｔｈｏｒ： Ｑｉｎ Ｊｕｎ， Ｅ⁃ｍａｉｌ： ｑｉｎｊｕｎ９８０１＠ １２６． ｃｏｍ
Ｆｕｎｄｉｎｇ ｐｒｏｇｒａｍ： Ｋｅｙ Ｓｃｉｅｎｔｉｆｉｃ Ｒｅｓｅａｒｃｈ Ｐｒｏｇｒａｍ ｉｎ Ｄｅｐａｒｔｍｅｎｔ ｏｆ Ｅｄｕｃａｔｉｏｎ， Ｈｕｂｅｉ Ｐｒｏｖｉｎｃｉａｌ （ Ｄ２０１４２１０４）
【 Ａｂｓｔｒａｃｔ】 Ｏｂｊｅｃｔｉｖｅ To investigate the ratio of peripheral blood helper T cells 17 (Th17 cells ) to regulatory T
cells (Treg cells) and the level of urinary monocyte chemoattractant protein⁃1 (MCP⁃1) in patients with systemic lupus ery⁃
thematosus (SLE). Ｍｅｔｈｏｄｓ A total of 68 patients with SLE admitted to Shiyan People's Hospital from July 2018 to Sep⁃
tember 2020 were selected and divided into a non lupus nephritis group of 28 patients (non⁃LN group) and a lupus nephritis
group of 40 patients (LN group) based on whether or not the SLE patients had renal involvement. During the same period,
35 healthy physical examinees from the hospital were included in the healthy control group. Collect clinical data of subjects,
use flow cytometry to detect the ratio of Th17 cells to Treg cells in peripheral blood mononuclear cells (PBMC), and use RT⁃
PCR technology to detect the corresponding transcription factor retinoic acid related solitary nuclear receptor (ROR⁃ γ t) of
Th17 cells and Treg cells)， and the expression of forked helical wing like transcription factor 3 (Foxp3) mRNA. Urine MCP⁃
·０２４· 疑难病杂志 ２０２３ 年 ４ 月第 ２２ 卷第 ４ 期 Ｃｈｉｎ Ｊ Ｄｉｆｆｉｃ ａｎｄ Ｃｏｍｐｌ Ｃａｓ，Ａｐｒｉｌ ２０２３，Ｖｏｌ． ２２，Ｎｏ． ４

1 levels were measured using ELISA. Spearman correlation was used to analyze the relationship between Th17/Treg cell ratio
and urine MCP⁃1, as well as its correlation with clinical indicators. Subjects' performance characteristic curve (ROC) was used
to analyze Th17/Treg cell ratio and urine MCP⁃1 levels in the diagnosis of LN in patients with SLE. Ｒｅｓｕｌｔｓ The ratio of
Th17 cells in LN group and non LN group was higher than that in healthy control group (
F = 12.345,
P = 0.015). The ratio of
Treg cells in LN group < non⁃LN group < healthy control group (
F = 22.388,
P < 0.001), and the ratio of Th17/Treg cells in LN
group > non⁃LN group > healthy control group (
F = 26.674,
P < 0.001); Comparison of ROR⁃ γ t mRNA expression levels in
LN group > non⁃LN group > healthy control group (
F = 20.578,
P < 0.001). The expression level of Foxp3 mRNA in LN group
and non⁃LN group was lower than that in healthy control group (
F = 7.782,
P = 0.003); Comparison of urinary MCP⁃1 levels in
LN group > non⁃LN group > healthy control group (
F = 51.231,
P < 0.001). Spearman correlation analysis showed that there
was a positive correlation between the Th17/Treg cell ratio and the urine MCP⁃1 level in SLE patients (
r = 0.604,
P = 0.017), a
positive correlation between the Th17/Treg cell ratio and the SLEDAI score in SLE patients (
r = 0.534,
P = 0.021), and a posi⁃
tive correlation between the urine MCP⁃1 level and the SLEDAI score and 24⁃hour urine protein content (
r/P = 0.342/ < 0.001,
0.412/0.023). The ROC curve showed that the area under curve (AUC) of Th17/Treg cell ratio and urine MCP⁃1 in predicting
LN in SLE patients was 0.718 and 0.769, with sensitivity of 0.782 and 0.993, and specificity of 0.736 and 0.569.Ｃｏｎｃｌｕｓｉｏｎ
In patients with SLE and LN, there is an imbalance in the ratio of Th17/Treg cells and an increase in the level of MCP⁃1 in
urine. The combined detection of the two can be used as an indicator of disease activity in SLE patients, and is helpful in
assessing the progress of LN.
【 Ｋｅｙ ｗｏｒｄｓ】 Systemic lupus erythematosus; Lupus nephritis;T help cell 17; T regulation cell; Monocyte chemotac⁃
tic protein⁃1