Ｄｉａｇｎｏｓｔｉｃ ｖａｌｕｅ ｏｆ ｃｅｒｖｉｃａｌ ｌｉｑｕｉｄ⁃ｂａｓｅｄ ｃｙｔｏｌｏｇｙ ｐ１６ ａｎｄ ｉｔｓ ｃｏｍｂｉｎａｔｉｏｎ ｗｉｔｈ ｈｉｇｈ⁃ｒｉｓｋ ＨＰＶ ｄｅｔｅｃｔｉｏｎ ｉｎ ｓｃｒｅｅｎｉｎｇ
ｃｅｒｖｉｃａｌ ｃａｎｃｅｒ ａｎｄ ｐｒｅｃａｎｃｅｒｏｕｓ ｌｅｓｉｏｎｓ Ｚｈａｏ Ｆａｎｇ∗ ， Ｍａ Ｄｅｙｏｎｇ， Ｗａｎｇ Ｔｉｎｇｔｉｎｇ， Ｚｈａｎｇ Ｙａｎ， Ｄｏｎｇ Ｙｉｎｇ， Ｚｈａｏ
Ｊｉａｎ． ∗ Ｄｅｐａｒｔｍｅｎｔ ｏｆ Ｏｂｓｔｅｔｒｉｃｓ ａｎｄ Ｇｙｎｅｃｏｌｏｇｙ， Ｂｅｉｊｉｎｇ Ｊｉｓｈｕｉｔａｎ Ｈｏｓｐｉｔａｌ，Ｂｅｉｊｉｎｇ １０００３５，Ｃｈｉｎａ
Ｃｏｒｒｅｓｐｏｎｄｉｎｇ ａｕｔｈｏｒ： Ｚｈａｏ Ｊｉａｎ， Ｅ⁃ｍａｉｌ： ｊｉａｎｚｈａｏ＠ ｐｋｕ． ｅｄｕ． ｃｎ
Ｆｕｎｄｉｎｇ ｐｒｏｇｒａｍ： Ｎａｔｉｏｎａｌ Ｋｅｙ Ｒｅｓｅａｒｃｈ ａｎｄ Ｄｅｖｅｌｏｐｍｅｎｔ Ｐｌａｎ Ｆｕｎｄｅｄ Ｐｒｏｊｅｃｔ （２０２０ＡＡＡＡ０１０５２００

Abstract：

Objective To evaluate the diagnostic value of cervical liquid-based cytology detection p16 and its combination with high-risk human papillomavirus(HR-HPV) detection in screening cervical cancer and precancerous lesions. Methods Select 900 women who received liquid-based cytology p16, liquid-based cytology(LBP) and high-risk HPV test in the Department of Obstetrics and Gynecology of Peking University First Hospital from January 2018 to August 2019 to analyze the screening results. Positive patients were referred to colposcopy biopsy. According to the pathological results of biopsy, evaluate the recognition efficiency of different screening strategies for high-level cervical lesions. Results The positive rate of p16 expression increased with the severity of cytology and the severity of cervical lesions. P16 has the highest sensitivity and negative predictive value for identifying high-level lesions, 0.969 and 0.995 respectively. LBP has the lowest sensitivity(0.853), but the highest specificity(0.859). HR-HPV positive predictive value and accuracy rate were the lowest, 0.328 and 0.700 respectively, with statistically significant difference(P<0.05). the="" sensitivity="" of="" lbp="" and="" hpv="" combined="" screening="" modes="" was="" 0.960="" 0.992="" respectively.="" specificity="" 0.634="" 0.643="" with="" no="" significant="" p="">0.05). Conclusion p16 and p16+HPV alone can effectively identify high-level cervical lesions. p16 is convenient and easy to operate, helps to simplify the screening strategy, and has better promotion and practical clinical application value.

High-grade cervical lesions;                    Cervical cancer;                    Biomarkers;                    Liquid based cytology test;                    p16;                    HR-HPV;