Objective To observe the effects of 3,3 '-diindolylmethane(DIM) on the proliferation and oxidative stress of H9c2 cells induced by Lipopolysaccharide(LPS). Methods From October to December 2022, the experiment was carried out in Hubei Key Laboratory of Metabolism and Related Chronic Diseases, People's Hospital of Wuhan University. H9c2 cells were stimulated with LPS(10 mg/ml) and different concentrations of DIM(10, 20, 30 μ Mol/L) for intervention. The live cell counting kit(CCK-8) was used to detect the proliferation ability of H9c2 cells; By detecting intracellular reactive oxygen species(ROS) levels and analyzing the toxic effects of LPS on H9c2 cells. Results The CCK-8 results suggest that DIM has no significant effect on the activity of H9c2 cells(P>0.05); LPS can increase the level of ROS in H9c2 cells. When DIM and LPS act simultaneously, ROS levels are significantly reduced(LPS+DIM Ⅰ group: F=27.44, P<0.001, LPS+DIM Ⅱ group: F=14.06, P<0.001, LPS+DIM Ⅲ group: F=18.76, P<0.001); LPS can enhance the proliferation activity of H9c2 cells(F=28.62, P<0.01), and when DIM and LPS act simultaneously, it can significantly reduce the increase in proliferation activity of H9c2 cells by LPS(LPS+DIM I group: F=7.451, P=0.002, LPS+DIM Ⅱ group: F=8.822, P=0.001, LPS+DIM Ⅲ group: F=10.460, P=0.001). Conclusion DIM can inhibit LPS induced oxidative stress in H9c2 cells and improve the proliferative activity of H9c2 cells.

A family follow-up and literature review of ataxia telangiectasia with ATM gene mutation were reported.

The clinical data of a patient with serous cystadenoma of fallopian tube combined with supernumerary ovary and fallopian malformations were reported and literature review was conducted.

Programmed death receptor-1(PD-1) and its ligands PD-L and PD-L2 play an important role in the immune response. PD-1/PD-L1 or PD-L2 signal pathway has been extensively studied in cancer immunity, and is crucial in regulating infection, autoimmunity and allergic inflammation. Studies have shown that PD-L1 and PD-L2 can regulate the activation and function of T cells and the start or stop of adaptive immune response. This review summarized the basic characteristics of PD-1/PD-L1 or PD-L2 signaling pathway, its relationship with immune tolerance and its role in bronchial asthma.

Cervical cancer is the most common malignant tumor of female lower reproductive tract. At present, it is recognized that the continuous infection of high-risk human papillomavirus(HR-HPV) is the main cause of its incidence. For the prevention and treatment of cervical precancerous lesions and the prevention of cervical cancer, it is important to explore the mechanism of persistent infection of HR-HPV. Studies have shown that HR-HPV infection is related to vaginal micro ecological environment, such as vaginal microorganisms and vaginal local immune system. This paper deeply explores the correlation between vaginal micro ecological environment and HR-HPV infection, in order to find new ideas for the prevention and treatment of HR-HPV persistent infection, and provide a theoretical basis for further exploring the treatment of cervical cancer.