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《YiNanBing ZaZhi》2023 Vol.22,No.3
  • Expression level and clinical significance of miR-34a-5p and miR-149-5p in joint fluid of patients with knee osteoar- thritis
    Author:Wang Hao,Tu Feng,Zhao Wenbin,Zhang Lin keyword:Knee osteoarthritis; miR-34a-5p; miR-149-5p; Synovial fluid
    Objective To analyze the expression and clinical significance of miR-34a-5p and miR-149-5p in joint
    fluid of patients with knee osteoarthritis ( KOA) . Methods The clinical data of 90 KOA patients from the Orthopedics De-
    partment of Wuhan First Hospital from January 2020 to June 2022 were collected. According to the Kellgren-Lawrence ( KL)
    standard grading of X-ray film,45 patients were divided into mild KOA group ( grade 1-2 patients) and severe KOA group
    ( grade 3-4 patients) . In addition,45 patients with physical examination in the same period were selected as the control
    group. The expression of miR-34a-5p and miR-149-5p in joint fluid of each group was detected by qRT-PCR,and the amount
    of expression IL-1β in joint fluid of each group was detected by ELISA. Spearman analyzed the correlation between expression
    level of miR-34a-5p,miR-149-5p and IL-1β in joint fluid,KL grading. The diagnostic value of the expression of miR-34a-5p
    and miR-149-5p in the joint fluid for the severity of KOA was analyzed by the working characteristic curve ( ROC) of subjects.
    Results MiR-34a-5p,IL-1 in knee joint fluid β Comparison of expression level,severe KOA group > mild KOA group > con-
    trol group ( F / P = 19. 21 / < 0. 001,14. 86 / < 0. 001) . Compared with the expression level of miR-149-5p in knee joint fluid,
    severe KOA group < mild KOA group < control group ( F / P = 63. 81 / < 0. 001) . Spearman correlation analysis showed that the
    expression level of miR-34a-5p and IL-1 β,KL grading showed significant positive correlation ( r / P = 0. 835 / < 0. 001,0.Chin J Diffic and Compl Cas,March 2023,Vol. 22,No. 3

    835 / < 0. 001) ,and the expression level of miR-149-5p was correlated with IL-1 β,KL grading showed significant negative
    correlation ( r / P = - 0. 828 / < 0. 001,- 0. 943 / < 0. 001) . The ROC curve analysis showed that the area under the curve of
    miR-34a-5p,miR-149-5p and their combined prediction of KOA severity were 0. 902,0. 850 and 0. 976,respectively. The
    value of their combined prediction of KOA severity was higher than the single index ( Z / P = 240. 70 /0. 046224. 13 /0. 005) .
    Conclusion The expression of miR-34a-5p and miR-149-5p in the joint fluid of KOA patients is abnormal,and is significant-
    ly related to the clinical grade and laboratory indicators of KOA,which can be used as a potential marker for diagnosis and mo-
    nitoring of KOA disease activity.
  • Health state identification guided by the theory of “Eight Character Classic of Tongluo Regimen”
    Author:Li Hongrong* , Wei Cong,Chang Liping,Yin Yujie,Jia Zhenhua keyword:“Eight-character theory of Tongluo Regimen”; Luo; Jing; Xing; Shen; Health status identification; Health management
    Health status identification is the basis and key link of implementing health management. Guided by the
    eight-character meridian theory of dredging collaterals and preserving health,this paper puts forward the identification of the
    healthy state of collaterals,essence,shape and spirit; Differentiation of essence: deficiency of kidney essence,deficiency of
    essence and bone,deficiency of essence and brain failure,deficiency of essence and lack of blood; Differentiation of shape:
    shape of collaterals and internal shape of collaterals; Differentiation of mind: mental injury and depression,mental anxiety,
    mental fatigue and forgetfulness,mental decline and insomnia. The physical and mental health status of sub-healthy and chro-
    nic disease people was comprehensively analyzed and evaluated in four aspects of collaterals,essence,shape and spirit by u-
    sing traditional Chinese medicine diagnostic methods and modern diagnostic techniques,providing the premise and basis for
    the application of the“Eight-character theory of Tongluo Regimen”to guide the implementation of health management.
  • Autosomal dominant mental retardation type 22 caused by ZBTB18 gene mutation: a case report and literature re- view
    Author: Li Yi* ,Zhou Zheng,Guo Zhidan,Lu Xiangpeng keyword: 22 type of autosomal dominant mental retardation; ZBTB18 gene; Mental retardation; Motor retardation; Backward language development
    This paper reported a case of autosomal dominant mental retardation type 22 caused by ZBTB18 gene muta-
    tion,and reviewed literatures
  • hrotic syndrome with syphilis and anticardiolipin antibody: a case report
    Author:Li Yan,Xue Chao,Li Wei. keyword: Nephrotic syndrome; Syphilis; Anticardiolipin antibody; Diagnosis; Treatment
    The paper reported a case of nephrotic syndrome with syphilis and anticardiolipin antibody,and reviewed
    the related literatures.
  • Application and research progress of deciduous tooth pulp stem cells in nervous system diseases
    Author: Zhou Shaolan,Li- ang Yan,Yuan Yuanyuan keyword:Nervous system disease; Dental stem cell; Tissue repair; Nerve regeneration
    Neurodegenerative diseases are a kind of diseases characterized by neurodegenerative diseases. At present,
    they occur frequently all over the world,and there is no effective treatment. Pulp stem cells of deciduous teeth are derived
    from the pulp of deciduous teeth and from the neural crest. They have high neurogenic potential and can be used to treat a va-
    riety of nervous system diseases. This article reviews the application and research progress of deciduous tooth pulp stem cells
    ( SHEDs) in tissue repair and regeneration of nervous system diseases.