Objective To explore the clinical value of serum endoplasmic reticulum aminopeptidase 1(ERAP1),adenosine deaminase acting on RNA 1(ADAR1), and zinc finger protein 132(ZNF132) in predicting lymph node metastasis and prognosis in patients with colorectal cancer. Methods A total of 185 colorectal cancer patients consecutively enrolled between July 2019 and October 2020 at the Department of Gastrointestinal Surgery, First Affiliated Hospital of Baotou Medical College, were included in the case group. Patients were categorized into a metastatic subgroup(94 cases) and a non-metastatic subgroup(91 cases) based on the presence of lymph node metastasis. Patients were further divided into a survival subgroup(122 cases) and a mortality subgroup(63 cases) based on their survival status. Additionally, 100 individuals who underwent health examinations at the hospital were recruited as the healthy control group. A Cox proportional hazards regression model was used to analyze prognostic factors affecting colorectal cancer patients. Kaplan-Meier survival curve analysis was used to examine the relationship between serum ERAP1 m RNA, ADAR1 m RNA, and ZNF132 m RNA levels and five-year survival outcomes. ROC curve analysis was used to evaluate the prognostic predictive value of serum ERAP1 m RNA, ADAR1 m RNA,and ZNF132 m RNA in colorectal cancer patients. Results Compared with the healthy control group, the case group exhibited significantly decreased serum levels of ERAP1 m RNA and ZNF132 m RNA, and significantly increased ADAR1 m RNA levels(t/P = 17.263/<0.001, 26.671/<0.001, 29.485/<0.001). Compared with the non-metastatic subgroup, the metastatic subgroup showed significantly decreased serum ERAP1 m RNA and ZNF132 m RNA levels, and significantly increased ADAR1 m RNA levels(t/P = 7.638/<0.001, 7.797/<0.001, 5.209/<0.001). Compared with the survival subgroup, the mortality subgroup had significantly higher proportions of clinical stage Ⅲ–Ⅳ, poorly differentiated tumors, and lymph node metastasis, as well as significantly increased serum ADAR1 m RNA levels and decreased ERAP1 m RNA and ZNF132 m RNA levels(χ2/t/P = 33.687/<0.001, 13.618/<0.001, 16.248/<0.001, 7.275/<0.001, 8.148/<0.001, 7.567/<0.001). Clinical stage Ⅲ–Ⅳ, lymph node metastasis, and elevated ADAR1 m RNA expression were independent risk factors for mortality in colorectal cancer patients, while increased ERAP1 m RNA and ZNF132 m RNA expression were independent protective factors [HR(95% CI) = 2.589(1.975-3.393), 2.115(1.288-3.473), 2.437(1.538-3.863), 0.346(0.201-0.597), 0.365(0.200-0.666)]. The five-year overall survival rates of patients with low serum ERAP1 m RNA and ZNF132 m RNA expression were significantly lower than those of patients with high expression(χ2= 10.245, 10.274; both P<0.01). Similarly, the five-year overall survival rate of patients with high serum ADAR1 m RNA expression was significantly lower than that of patients with low expression(χ2= 7.782;P<0.01).The AUC values for serum ERAP1 m RNA, ADAR1 m RNA, ZNF132 m RNA, and their combination in predicting poor prognosis in colorectal cancer patients were 0.832, 0.807, 0.803, and 0.925, respectively, with the combined AUC being the highest(Z = 3.177, 3.480, 3.782; P = 0.002, 0.001,<0.001). Conclusion Serum ERAP1 and ZNF132 levels are decreased, while ADAR1 level is increased, in patients with colorectal cancer. These changes are associated with lymph node metastasis and poor prognosis. Combined detection of serum ERAP1, ADAR1, and ZNF132 has predictive value for the prognosis of patients with colorectal cancer.

Objective To explore the correlation between serum and glucocorticoid-induced kinase 1(SGK1), kallikrein 7(KLK7), and adiponectin(ADPN) levels and glucose and lipid metabolisms and insulin resistance(IR) in patients with type 2 diabetes mellitus(T2 DM) complicated with metabolic syndrome(MS). Methods A total of 229 T2 DM patients admitted to the Department of Endocrinology and Diabetes II at Cangzhou Central Hospital from August 2021 to August 2024 were selected as the study subjects. According to whether the patients had MS, they were divided into an MS group and a nonMS group. Pearson correlation analysis was used to examine the relationships between serum SGK1, KLK7, ADPN and glucose-lipid metabolism and IR. Logistic regression analysis was performed to identify factors influencing MS in T2 DM patients.ROC curve analysis was conducted to evaluate the value of serum SGK1, KLK7, and ADPN in assessing MS in T2 DM patients. Results Compared with the non-MS group, the MS group showed significantly increased FBG, 2 hPBG, TG, TC,LDL-C, HOMA-IR, and HOMA-β, and significantly decreased HDL-C(t/P = 12.492/<0.001, 10.676/<0.001, 27.963/<0.001,21.813/<0.001, 17.965/<0.001, 23.899/<0.001, 11.115/<0.001, 12.638/<0.001). Serum SGK1 and KLK7 levels in the MS group were significantly higher than those in the non-MS group, while ADPN levels were significantly lower(t/P = 20.657/<0.001, 14.955/<0.001, 18.211/<0.001). Serum SGK1 and KLK7 were positively correlated with FBG, 2 hPBG, TG, TC, LDLC, HOMA-IR, and HOMA-β, and negatively correlated with HDL-C(r/P = 0.373/<0.001, 0.410/<0.001, 0.364/<0.001, 0.342/<0.001, 0.436/<0.001, 0.451/<0.001, 0.416/<0.001, 0.413/<0.001, 0.364/<0.001, 0.382/<0.001, 0.386/<0.001, 0.408/<0.001,0.423/<0.001,-0.402/<0.001,-0.363/<0.001). ADPN was negatively correlated with FBG, 2 hPBG, TG, TC, LDL-C, HOMAIR, and HOMA-β, and positively correlated with HDL-C(r/P =-0.436/<0.001,-0.431/<0.001,-0.401/<0.001,-0.349/<0.001,-0.498/<0.001,-0.439/<0.001,-0.408/<0.001, 0.465/<0.001). High BMI, large waist circumference, low hip circumference, high waist-to-hip ratio, high SGK1, and high KLK7 were independent risk factors for T2 DM patients with MS(all P<0.05), while high ADPN was an independent protective factor [OR(95%CI) = 2.432(1.438-4.112), 3.015(1.862-4.883),2.965(1.612-5.454), 3.164(1.622-6.173), 2.654(1.573-4.479), 3.012(1.641-5.530), 0.413(0.233-0.735)]. The AUCs of serum SGK1, KLK7, ADPN, and their combined assessment for diagnosing MS in T2 DM patients were 0.813, 0.809, 0.790,and 0.936, respectively, with the combined assessment showing the highest AUC(Z/P = 2.726/0.007, 2.732/0.006, 2.741/0.004).Conclusion There are abnormal changes in the levels of serum SGK1, KLK7, and ADPN in patients with T2 DM complicated with MS. All three markers are related to glucose and lipid metabolism and IR. Combined detection has certain clinical value for evaluating T2 DM complicated with MS.

Objective To characterize local oral microecological dysbiosis and mi R-34a expression in chronic periodontitis(CP) associated with type 2 diabetes mellitus(T2 DM).Methods A total of 63 patients with CP concomitant with T2 DM were included as the study group, and 63 patients with CP alone were selected at a 1:1 ratio as the control group. All participants were enrolled from January 2021 to December 2023 in the Department of Stomatology, Nanchong Hospital of Beijing Anzhen Hospital, Capital Medical University/Nanchong Central Hospital, Sichuan, China. Periodontal clinical indices, the composition of pathogenic bacteria in saliva and gingival crevicular fluid(GCF), and mi R-34a expression levels in saliva and GCF were compared between the two groups.Results Compared with the control group, the study group had higher plaque index, gingival bleeding index, clinical attachment loss, and probing depth(t = 2.923, 10.924, 6.283, 4.555;P = 0.004,P<0.001,P<0.001,P<0.001). The abundances of Porphyromonas gingivalis, Capnocytophaga spp., Prevotella intermedia, and Prevotella nigrescens were increased in saliva(t = 2.242, 2.316, 2.682, 2.506; P = 0.027, P = 0.022, P = 0.008, P = 0.014) and in GCF(t =2.457, 2.713, 5.513, 4.836; P = 0.015, P = 0.008, P<0.001, P<0.001). Relative mi R-34a expression levels in saliva and GCF were also elevated(t = 5.885 and 3.269; both P<0.001). Multivariable logistic regression analysis showed that high mi R-34a expression and high P. gingivalis load were associated with T2 DM-related chronic periodontitis [OR(95%CI) = 2.454(1.283-4.704) and 2.112(1.125-3.971)].Conclusion CP patients with T2 DM present more severe periodontal destruction, accompanied by enrichment of key periodontal pathogens and upregulated mi R-34a expression. These findings suggest that diabetes may exacerbate periodontal lesions through microecological and molecular mechanisms, warranting further investigation.

Objective To construct and apply a daytime surgery model of flexible ureteroscopic lithotripsy(RIRS)under the concept of enhanced recovery after surgery(ERAS), providing reference guidance for the implementation of daytime surgery. Methods A total of 100 patients with kidney stones or ureteral stones admitted to the Department of Urology of Dongguan People' s Hospital from September 2022 to December 2023 were selected. According to the surgical methods, they were divided into the ERAS group(receiving ERAS daytime RIRS surgery, n = 50) and the traditional group(receiving traditional RIRS surgery, n = 50). Perioperative indicators, blood biochemical indicators, renal function, psychological status, and complications were compared between the two groups. Results The operation time and the time to getting out of bed for activity in the ERAS group were significantly shorter than those in the traditional group(t/P = 2.025/0.046, 6.081/<0.001). There was no statistically significant difference in the primary stone clearance rate between the two groups(χ2/P = 0.457/0.499). The levels of urinary white blood cell count, red blood cell count(RBC), blood white blood cell count, and C-reactive protein(CRP) in the ERAS group on postoperative day 1(T1), day 3(T2), and day 30(T3) were significantly lower than those in the traditional group(t/P = 4.327/<0.001, 6.670/<0.001, 21.240/<0.001, 2.217/0.029, 3.186/0.002, 3.293/0.001, 2.196/0.030, 3.660/<0.001, 3.898/<0.001, 3.527/<0.001, 2.292/<0.001, 5.063/<0.001). The glomerular filtration rate(GFR) level at T3 in the ERAS group was significantly higher than that in the traditional group, while the levels of blood urea nitrogen(BUN) and serum creatinine(Scr) were significantly lower(t/P = 14.502/<0.001, 3.670/<0.001, 7.237/<0.001). The scores of the Self-Rating Anxiety Scale(SAS) and the Self-Rating Depression Scale(SDS) at T3 in the ERAS group were significantly lower than those in the traditional group(t/P = 4.596/<0.001, 5.937/<0.001). The complication rates of the ERAS group and the traditional group were8.00% and 14.00%, respectively, with no statistically significant difference(χ2/P = 0.919/0.338). Conclusion The application of the ERAS-based daytime surgery model in RIRS can shorten surgical recovery time, reduce inflammatory responses, optimize renal function, and improve psychological status in patients.

Objective To explore and analyze the relationship between the expression of serum thioredoxin-1(Trx1),melatonin and the NOD-like receptor family pyrin domain containing 3(NLRP3) inflammasome, the severity of the disease,and prognosis in patients with pregnancy-induced hypertension(PIH). Methods From March 2022 to March 2025, 130 patients with PIH who were treated and delivered in the Department of Obstetrics, Guangxi Zhuang Autonomous Region People's Hospital were selected and classified into a mild group(64 cases), a moderate group(45 cases), and a severe group(21 cases). According to prognosis, they were assigned into a good prognosis group(98 cases) and an adverse prognosis group(32 cases). General clinical data and serum Trx1 and melatonin levels were collected and analyzed. Multivariate logistic regression was used to explore prognostic factors for patients with PIH. ROC curve analysis was used to evaluate the predictive value of serum Trx1 and melatonin for the prognosis of patients with PIH. Correlations between serum Trx1, melatonin and NLRP3 inflammasome, as well as disease severity, were analyzed using Spearman and Pearson methods. Results Patients in the adverse prognosis group exhibited significantly lower serum Trx1 and melatonin levels compared to the good prognosis group(t/P = 7.590/<0.001, 7.731/<0.001). Serum Trx1 and melatonin levels were significantly higher in the mild group than in the moderate group, and significantly higher in the moderate group than in the severe group(F/P = 10.726/<0.001, 63.283/<0.001).Trx1 and melatonin levels showed a negative correlation with disease severity(r/P =-0.502/<0.001,-0.533/<0.001). Serum Trx1 and melatonin levels also exhibited a negative correlation with NLRP3 expression(r/P =-0.486/<0.001,-0.473/<0.001).Elevated NLRP3 level was an independent risk factor for poor prognosis in PIH patients [OR(95%CI) = 1.869(1.067-3.274)]. Conversely, elevated Trx1 and elevated melatonin levels were independent protective factors against poor prognosis[OR(95%CI)= 0.798(0.647-0.984), 0.795(0.641-0.986)]. The AUC values for predicting poor prognosis in PIH patients using Trx1, melatonin, and their combination were 0.825, 0.796, and 0.907, respectively. The AUC for the combination was significantly higher than that for either single indicator(Z/P = 2.241/0.025, 2.902/0.004). Conclusion Serum Trx1 and melatonin levels are decreased in patients with adverse prognosis of PIH. These levels are correlated with disease severity and NLRP3 expression, and have auxiliary predictive value for pregnancy outcomes in these patients.