Ｓｅｒｕｍ ｅｘｐｒｅｓｓｉｏｎ ｏｆ β⁃ｃａｔｅｎｉｎ ａｎｄ ｌｎｃＲＮＡ ＫＣＮＱ１ＯＴ１ ａｎｄ ｔｈｅ ａｐｐｌｉｃａｔｉｏｎ ｉｎ ｔｈｅ ｄｉａｇｎｏｓｉｓ ｏｆ ｌｉｖｅｒ ｆｉｂｒｏｓｉｓ ｉｎ ｐａ⁃
ｔｉｅｎｔｓ ｗｉｔｈ ｃｈｒｏｎｉｃ ｈｅｐａｔｉｔｉｓ Ｂ Ｓｕｎ Ｒｕｉｑｉｎｇ， Ｄｅｎｇ Ｊｕａｎ， Ｌｉ Ｆｅｎｇ， Ｗａｎｇ Ｌｏｎｇ， Ｗａｎｇ Ｘｉｕｆａｎｇ． Ｄｅｐａｒｔｍｅｎｔ ｏｆ Ｇａｓｔｒｏｅｎｔｅｒ⁃
ｏｌｏｇｙ， ｔｈｅ Ｆｉｒｓｔ Ａｆｆｉｌｉａｔｅｄ Ｈｏｓｐｉｔａｌ ｏｆ Ａｉｒ Ｆｏｒｃｅ Ｍｉｌｉｔａｒｙ Ｍｅｄｉｃａｌ Ｕｎｉｖｅｒｓｉｔｙ，Ｓｈａａｎｘｉ Ｐｒｏｖｉｎｃｅ，Ｘｉ’ ａｎ ７１００３２， Ｃｈｉｎａ
Ｃｏｒｒｅｓｐｏｎｄｉｎｇ ａｕｔｈｏｒ： Ｗａｎｇ Ｘｉｕｆａｎｇ， Ｅ⁃ｍａｉｌ：ｗｘｆｍｍ＠ １６３． ｃｏｍ
【Ａｂｓｔｒａｃｔ】 Ｏｂｊｅｃｔｉｖｅ To investigate the expression of β⁃catenin and long chain non coding RNA (lncRNA) KC⁃
NQ1OT1 in serum of patients with chronic hepatitis B and their application value in the diagnosis of liver fibrosis. Ｍｅｔｈｏｄｓ
A total of 90 patients with chronic hepatitis B admitted to the First Affiliated Hospital of Air Force Military Medical Uni⁃
versity from January 2020 to January 2021 were selected. According to the degree of liver fibrosis, they were divided into
23 patients without liver fibrosis (S0 group), 42 patients with mild liver fibrosis (S1 － S2 group), and 25 patients with mod⁃
erate to severe liver fibrosis (S3 － S4 group); In addition, 30 patients undergoing physical examination in the same hospital
were selected as the healthy control group. Compare the serum expression levels of β⁃catenin and lncRNA KCNQ1OT1
and indicators of liver fibrosis; analysis the correlation between the expression levels of β⁃catenin and lncRNA KC⁃
NQ1OT1 and the degree of liver fibrosis and its indicators; Logistic regression analysis was used to analyze the influen⁃
cing factors of liver fibrosis in patients with chronic hepatitis B; receiver operation characteristic curve (ROC) analysis the
predictive value of serum β⁃catenin and lncRNA KCNQ1OT1 levels in the development of liver fibrosis in patients with
·２０４· 疑难病杂志 ２０２３ 年 ４ 月第 ２２ 卷第 ４ 期 Ｃｈｉｎ Ｊ Ｄｉｆｆｉｃ ａｎｄ Ｃｏｍｐｌ Ｃａｓ，Ａｐｒｉｌ ２０２３，Ｖｏｌ． ２２，Ｎｏ． ４

chronic hepatitis B. Ｒｅｓｕｌｔｓ Serum β⁃catenin and lncRNA KCNQ1OT1 levels in healthy control group, S0 group, S1⁃S2
group, S3⁃S4 group increased sequentially (
F/P = 28.066/ < 0.001, 25.173/ < 0.001). The levels of hyaluronic acid (HA), pro⁃
collagen N⁃terminal peptide (P III P), type IV collagen (C IV), and laminin (LN) were gradually increased (
F/P = 79.414/ <
0.001, 119.630/ < 0.001, 104.574/ < 0.001, 96.631/ < 0.001). The levels of serum β⁃catenin and lncRNA KCNQ1OT1 were sig⁃
nificantly and positively correlated with the degree of liver fibrosis (
r/P = 0.598/0.001, 0.643/0.009). β⁃Catenin and lncRNA
KCNQ1OT1 levels were significantly positively correlated with liver fibrosis indicators HA, P III P, C IV, and LN（ β⁃cate⁃
nin：
r/P = 0.483/0.016, 0.456/0.013, 0.641/0.006, 0.519/0.008； Serum lncRNA KCNQ1OT1:
r/P = 0.496/0.014, 0.604/0.007, 0.523/
0.014, 0.611/0.002). Elevated levels of serum β⁃catenin, lncRNA, KCNQ1OT1, HA, P III P, C IV, and LN are risk factors for
liver fibrosis in patients with chronic hepatitis B ［
OR(95% CI) = 1.564 (1.205 － 2.030), 2.213 (1.449 － 3.379), 1.816 (1.261 －
2.615), 2.315 (1.380 － 3.884), 1.564 (1.161 － 2.107), and 3.013 (1.491 － 6.090)］ . The AUC of serum β⁃catenin, lncRNA, KC⁃
NQ1OT1, and their combination in predicting liver fibrosis in patients with chronic hepatitis B were 0.784, 0.775, and 0.857,
respectively. The combination of the two was superior to their respective prediction alone (
Z/P = 2.617/0.028, 2.897/0.014).
Ｃｏｎｃｌｕｓｉｏｎ The levels of serum β⁃catenin and lncRNA KCNQ1OT1 from patients with chronic hepatitis B are closely
related to the degree of liver fibrosis, and the combined detection of the two has a good reference value for the occur⁃
rence of liver fibrosis in patients with chronic hepatitis B.
【 Ｋｅｙ ｗｏｒｄｓ】 Hepatitis B,chronic; Hepatic fibrosis; β⁃catenin; Long non⁃coding RNA KCNQ1OT1; Diagnosis