The relationship between serum MIC-1, CGRP and Helicobacter pylori infection in patients with chronic gastritis and their clinical significance

Abstract:Objective To investigate the relationship and clinical significance between serum macrophage inhibitory factor-1(MIC-1), calcitonin gene-related peptide(CGRP) and Helicobacter pylori(Hp) infection in patients with chronic gastritis. Methods From June 2021 to June 2024, 104 patients diagnosed with chronic gastritis in our hospital were included as the chronic gastritis group. Based on the results of the 13 C urea breath test(13 C-UBT) breath test, they were assigned into the Hp subgroup(64 cases) and the non Hp subgroup(40 cases). Ninety healthy individuals were simultaneously selected as the healthy group. 13 C-UBT retesting was used to confirm the prognosis of eradication treatment in Hp subgroup patients. Enzyme linked immunosorbent assay(ELISA) was applied to detect serum levels of MIC-1, CGRP, and inflammatory factors interleukin-2(IL-2) and interleukin-6(IL-6). Pearson and Spearman correlation were applied to analyze the correlation between serum MIC-1, CGRP levels with IL-6, Hp infection, and prognosis. Multivariate Logistic regression was applied to analyze the influencing factors of Hp infection in patients with chronic gastritis. ROC was applied to analyze the predictive value of serum MIC-1 and CGRP for Hp infection in patients with chronic gastritis. Results The serum MIC-1 level in chronic gastritis group was higher than that in healthy control group, and the serum CGRP level was lower than that in healthy control group( t/P=15.529/<0.001, 33.596/<0.001). The serum IL-6 and MIC-1 levels of Hp subgroup were higher than those of non-Hp subgroups, and the serum IL-2 and CGRP levels were lower than those of non-Hp subgroups( t/P=23.282/<0.001, 5.501/<0.001, 5.968/<0.001, 6.925/<0.001). After Hp eradication treatment, the serum MIC-1 level in the non-eradication group was higher than that in the eradication group, and the serum CGRP level was lower( t/P=2.667/0.008, 6.646/<0.001). The serum MIC-1 level was positively correlated with IL-6 and Hp infection, and negatively correlated with prognosis( r/P=0.544/<0.001, 0.764/<0.001,-0.487/<0.001). The level of serum CGRP was negatively correlated with IL-6 levels and Hp infection, and positively correlated with prognosis( r/P=-0.572/<0.001,-0.654/<0.001, 0.682/<0.001). And positively correlated with prognosis. MIC-1, IL-6, and CGRP were influencing factors of Hp infection in patients with chronic gastritis[OR(95%CI)=0.835(0.750-0.901), 0.840(0.755-0.904), 0.913(0.841-0.959)]. Serum MIC-1 and CGRP could be used separately to diagnose Hp infection in patients with chronic gastritis, and the combined diagnostic effect of the two was greater than the individual predictive value( Z/P=2.342/0.019, 2.197/0.028). Conclusion Serum MIC-1, CGRP, and inflammatory factor IL-6 are all factors that affect the infection of Hp in patients with chronic gastritis. Among them, serum MIC-1 and CGRP can diagnose whether patients with chronic gastritis are infected with Hp, and the combination of the two has a better diagnostic effect.