Objective To analyze the expression of spinal protein 2(SPON2) in lung adenocarcinoma cells and its effect on the proliferation and metastasis of lung adenocarcinoma cells.Methods From January 2021 to April 2022, the experiment was carried out in Cangzhou Central Hospital. 86 lung adenocarcinoma tissues and their paired adjacent tissue samples were collected from January 2015 to December 2016, respectively. Immunohistochemical method(IHC) was used to detect the expression level of SPON2 in lung adenocarcinoma tissues and paired adjacent tissues, and analyze the relationship between the expression of SPON2 and clinical pathological parameters and prognosis of lung adenocarcinoma patients. Lung adenocarcinoma cells A549 were cultured and divided into si NC group(transfected with NC siRNA) and si SPON2 group(transfected with SPON2 siRNA). Western blot was used to detect the expression of SPON2, cyclin D1, cyclin dependent kinase 4(CDK4), matrix metalloproteinase 7(MMP-7), and MMP-9 in the two groups; CCK8 test and colony formation test were used to detect the proliferation ability of the two groups of cells; The cell cycle was detected by flow cytometry; Transwell test was used to detect the metastatic ability of the two groups of cells.Results Compared with the adjacent tissues, the positive expression rate of SPON2 in lung adenocarcinoma tissues was significantly increased(χ2=5.821,P=0.016). The expression of SPON2 in lung adenocarcinoma patients with T stage T3-4, N stage N1-2, TNM stage Ⅲ-Ⅳ was higher than that of T1-2, N0, Ⅰ-Ⅱ, respectively(χ2/P=7.481/0.006, 5.331/0.021, 9.139/0.003). Compared with lung adenocarcinoma patients with low expression of SPON2, lung adenocarcinoma patients with high expression of SPON2 have a poor prognosis, and the difference is statistically significant(χ2=4.877,P=0.027). The expression of SPON2 in si-SPON2 group was significantly higher than that in si-NC group(t=28.343,P<0.001). Compared with the si NC group, the proliferation and metastasis of A549 cells in the si SPON2 group decreased, the cell cycle was blocked in G0/G1 phase, and the expression of cyclinD1, CDK4, MMP-7, and MMP-9 decreased, with significant differences(P<0.05).Conclusion The up-regulated expression of SPON2 in lung adenocarcinoma may promote the proliferation and metastasis of lung adenocarcinoma by regulating cell cycle related proteins and metastasis related proteins, which may be a potential target for the treatment of lung adenocarcinoma.

Objective To explore the potential mechanism of Qiyue Tea in the treatment of rats with decreased ovarian reserve. Methods From March to May 2021, experiments will be conducted in the central laboratory of Dongzhimen Hospital, Beijing University of Traditional Chinese Medicine. Twenty-four 6-week-old SD rats were randomly divided into blank group, model group, Qiyue Tea group, and estradiol valerate tablet(Progalo) group, with 6 rats in each group. Except for the blank group, the other rats were intraperitoneally injected with VCD 160 mg/kg to establish a model of decreased ovarian reserve(DOR). The rats in the control group were given 0.12 mg/kg of Procyone by gavage, and the blank group and the model group were given 3 ml/kg of normal saline by gavage for 4 weeks. After the intervention, the serum levels of follicle-stimulating hormone(FSH), estradiol(E2) and anti-Müllerian hormone(AMH) were detected by ELISA. The protein expressions of Bax and Bcl 2 in the ovary were detected, and the expression level of Bcl 2/Bax in the ovary was detected by Western blotting.Results Compared with the blank group, the number of primary and secondary follicles in the model group decreased and the number of atretic follicles increased; the Qiyue Tea group and the Bujiale group had more primary and mature follicles, and fewer atretic follicles than the model group. Compared with the blank group, the ovarian BMI and AMH levels in the model group decreased, while the FSH level increased(P<0.05). FSH level decreased(P<0.05). Bax protein was mainly expressed in the granulosa cells, corpus luteum and interstitium. Compared with the blank group, the expression of Bax protein in the model group was higher than that in the blank group. The ratio of Bcl 2/Bax in the model group was lower than that in the control group(P<0.05); the ratio of Bcl 2/Bax in the Qiyue Tea group and Bujiale group was higher than that in the model group(P<0.05). Conclusion Qiyue Tea can reduce the level of FSH and increase the level of AMH in serum of DOR rats, which is related to down-regulating the expression of apoptosis factor Bax in ovarian tissue.

Objective The prognosis and influence of complete revascularization on patients with acute ST segment elevation myocardial infarction(STEMI) complicated with multi vessel disease were analyzed by systematic evaluation. Methods Search CNKI, Wanfang data, Vip Chinese database, PubMed, Embase, Cochrane Library and other databases until January 2021, and include relevant literature on randomized controlled trials for comparing the prognosis of complete revascularization and only criminal vascular interventional treatment of acute ST segment elevation myocardial infarction with multiple vascular diseases. After selecting literature and extracting data according to selection criteria, meta-analysis was conducted with Revman 5.3 software.Results Finally, 18 randomized controlled trials were included, with a total of 11 106 patients, including 4 680 patients in the complete revascularization group and 6 426 patients in the criminal vessel percutaneous coronary intervention group. Meta analysis showed that in the complete revascularization group, major cardiovascular events(MACE)(RR=0.60, 95%CI 0.52-0.71,P<0.001), all-cause death(RR=0.68,95% CI 0.58-0.81,P<0.001), cardiac death(RR=0.62, 95% CI 0.49-0.78,P<0.001), recurrent myocardial infarction(RR=0.78,95% CI 0.67-0.92,P=0.003), revascularization(RR=0.48, 95% CI 0.35-0.65,P<0.001) The incidence of recurrent angina pectoris(RR=0.66, 95% CI 0.47-0.93,P=0.02) was significantly lower than that in the criminal vessel PCI group, with a statistically significant difference; There was no significant difference between the two groups in the incidence of postoperative heart failure(RR=0.64, 95% CI 0.25-1.62,P=0.35), coronary artery bypass grafting(CAGB)(RR=0.64,95% CI 0.58-1.66,P=0.94), bleeding(RR=1.19, 95% CI 0.88-1.46,P=0.33), and contrast nephropathy(RR=1.59, 95% CI 0.94-2.71,P=0.18). Conclusion Complete revascularization treatment strategy can effectively reduce the incidence of MACE, all-cause death, cardiac death, recurrent myocardial infarction, revascularization, angina pectoris in STEMI patients with multi vessel disease, and can improve the prognosis to a certain extent, and it is safe and effective.

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