ObjectiveTo investigate the changes of lipid storage index(LAP) and visceral adipose index(VAI) in obstructive sleep apnea-hypopnea syndrome(OSAHS) and their predictive value for coronary heart disease(CHD).MethodsA total of 671 patients suspected of having coronary heart disease who were hospitalized at the Department of Hypertension, Cardiovascular Disease Center, the First Affiliated Hospital of Xinjiang Medical University between April 2020 and December 2023 were selected for this study. Based on whether there were complications of CHD, the patients were divided into two groups: CHD group(n=318) and the non-CHD group(n=353). LAP and VAI were calculated in all OSAHS patients. Logistic regression analysis was conducted to identify the influencing factors for OSAHS in patients with coronary heart disease, and the receiver operating characteristic(ROC) curve was utilized to evaluate the clinical predictive value of LAP and VAI in this context. Spearman correlation analysis was used to assess the correlation between various variables and the strength of the association between coronary heart disease risk factors and Gensini score. The results were respectively reflected in the heatmap and chord diagram.ResultsLAP and VAI of OSAHS patients in CHD group were higher than those in non-CHD group(t/P=12.084/< 0.001, 11.198/< 0.001).Logistic regression analysis showed that OSAHS with CHD was associated with male, age, hypertension, diabetes, AHI, LAP and VAI[OR(95%CI)=3.251(1.813-5.831), 3.027(1.954-4.690), 2.151(1.297-3.567), 2.253(1.366-3.715), 2.173(1.416-3.333), 2.530(1.421-4.503), 3.343(2.286-8.250)];The area under the curve(AUC) of LAP, VAI, and the combination of LAP and VAI for predicting CHD in patients with OSAHS were 0.773, 0.786, 0.788.The combined AUC of the two is greater than the AUC of LAP, and there is no statistical significance compared with the AUC predicted by VAI alone(Z/P=-2.590/0.010,-0.701/0.483). Spearman analysis showed that LAP was positively correlated with TG and VAI(r=0.97, 0.91), VAI was positively correlated with TG(r=0.93) and negatively correlated with HDL-C(r=-0.53).Male, age, hypertension, diabetes, AHI, LAP and VAI were positively correlated with Gensini score in OSAHS patients(r=0.149,0.253,0.126,0.204,0.196,0.325,0.336,P<0.001).ConclusionLAP and VAI are independent risk factors for OSAHS complicated with coronary heart disease, and they also exacerbate the severity of coronary heart disease. The combination of the two factors holds significant application value in the early diagnosis of OSAHS patients with comorbid CHD.

ObjectiveTo explore the early diagnostic value of spiral CT in combination with serum periostin and mannan-binding lectin-associated serine protease 2(MASP-2) for lung cancer.MethodsA total of 115 suspected lung cancer patients admitted to the Oncology Department of China Resources Wuhan Steel General Hospital Affiliated to Huatech University from June 2022 to August 2024 were selected as the study objects. All patients underwent spiral CT examination, and were divided into lung cancer group and benign lesion group using lung tissue biopsy results as the gold standard. The expression levels of serum periostin and MASP-2 were detected by ELISA. ROC curve was used to analyze the early diagnostic value of Spiral CT, periostin and MASP-2 for lung cancer patients. Kappa analysis was employed to analyze the consistency of spiral CT, serum periostin, MASP-2, and combined detection in diagnosing lung cancer with the gold standard. A four-cell table was drawn to analyze the early diagnostic value of spiral CT imaging detection, serum periostin, MASP-2, and combined detection for lung cancer.ResultsThe proportion of nodule size, "vacuole" sign, "aeration" sign, "lobed" sign and "burr" sign in lung cancer group was higher than that in benign lesion group(t/χ2/P=22.188/<0.001,5.351/0.021, 6.706/0.010, 10.823/0.001, 10.524/0.001). Compared with the benign lesion group, the serum Periostin and MASP-2 levels were up-regulated in the lung cancer group(t/P=7.925/<0.001, 7.185/<0.001). The AUC of spiral CT, sero-periostin, MASP-2 and their combination in the diagnosis of lung cancer were 0.896, 0.864, 0.844, and 0.970, respectively. The combined AUC of the three was superior to the diagnostic value of spiral CT, serperiostin, and MASP-2 respectively(Z/P=2.187/0.029, 4.310/<0.001, 3.943/<0.001). The diagnostic value of Periostin is superior to that of MASP-2. The diagnostic consistency between MASP-2 and Periostin and the gold standard for lung cancer was moderate, and the diagnostic consistency between spiral CT and the gold standard for lung cancer was strong. The combination of the three tests was in good agreement with the gold standard diagnosis of lung cancer(Kappa/P=0.604/<0.001, 0.587/<0.001, 0.460/<0.001, 0.816/<0.001). The accuracy, sensitivity, and negative predictive value of spiral CT, Periostin, and MASP-2 combined in the diagnosis of lung cancer were higher than those of Periostin and MASP-2 alone(P<0.05).ConclusionPeriostin and MASP-2 are upregulated in the serum of lung cancer patients, and the combination of spiral CT and serum Periostin and MASP-2 has a high diagnostic value for early lung cancer.

ObjectiveTo explore the value of the prediction model based on dynamic contrast-enhanced magnetic resonance imaging(DCE-MRI) parameters in predicting lymph node metastasis in non-small cell lung cancer(NSCLC).MethodsA retrospective study was conducted and 310 patients with NSCLC admitted to the Second People's Hospital of Hengshui in Hebei Province from March 2020 to February 2024 were enrolled in this study. All patients underwent DCE-MRI scan before surgery. According to surgical and pathological results, the subjects were divided into metastasis group(n=113) and non-metastasis group(n=197). General information, laboratory indexes and DCE-MRI parameters [volume transfer constant(K trans), extracellular extravascular space volume fraction(Ve), plasma volume fraction(V p) and rate constant(K ep)] of the two groups were compared. Multivariate logistic regression model was used to analyze the independent risk factors for lymph node metastasis in NSCLC. A risk prediction model was constructed based on the analysis results and the goodness of fit of the prediction model was evaluated by Homser-Lemeshow test. The receiver operating characteristic(ROC) curve was used to evaluate the efficacy of the model for predicting lymph node metastasis in NSCLC.ResultsPreoperative serum carcinoembryonic antigen(CEA), cytokeratin fragment antigen 21-1(CYFRA21-1), free prostate-specific antigen(f-PSA) and human growth hormone(HGH) levels in the metastasis group were higher than those in the non-metastasis group, while K trans and Ve were higher than those in the non-metastasis group(P<0.05). there="" was="" no="" significant="" difference="" in="" v="" p="" or="" k="" ep="" between="" the="" two="">0.05). Multivariate logistic regression analysis found that preoperative f-PSA, preoperative HGH, K trans and Ve were risk factors for lymph node metastasis(P<0.05). 2="6.287," a="" prediction="" model="" was="" constructed="" as="" follows:="" preoperative="" k="" ve.="" homser-lemeshow="" goodness="" of="" fit="" test="" showed="" p="">0.05. ROC curve analysis showed that the areas under the curve(AUCs) of K trans and Ve for predicting lymph node metastasis in NSCLC were 0.590 and 0.616, respectively. The AUC of the prediction model was 0.808, which was greater than that of each index(Z/P=5.824/<0.001,4.923/<0.001).ConclusionDCE-MRI parameters(K trans and Ve) are related to lymph node metastasis in NSCLC. The prediction model constructed by combining above parameters with preoperative f-PSA and preoperative HGH demonstrates good predictive efficacy for lymph node metastasis in NSCLC.

ObjectiveTo investigate the expression levels and prognostic value of serum decoy receptor 3(DcR3) and gasdermin D( GSDMD) in uremic hemodialysis patients.MethodsFrom January 2022 to June 2023, 170 patients with uremia hemodialysis admitted to the Department of Nephrology, Hanzhong 321 Hospital were selected as uremia group, and 170 patients with non-uremia chronic kidney disease( CKD) admitted to the same period were selected as control group. Uremic hemodialysis patients were divided into poor prognosis subgroup(62 cases) and good prognosis subgroup(108 cases) according to whether major adverse cardiovascular events(MACE) occurred after 1 year. Serum DcR3 and GSDMD levels were detected by enzyme-linked immunosorbent assay. Multivariate unconditional Logistic regression analysis was used to analyze the relationship between serum DcR3 and GSDMD levels and poor prognosis of uremic hemodialysis patients, the receiver operating characteristic curve was used to analyze the evaluation value of serum DcR3 and GSDMD levels.ResultsCompared with the control group, the levels of serum DcR3 and GSDMD in the uremia group were increased(t/P=11.414/<0.001, 12.994/<0.001). The poor prognosis rate of 170 uremic hemodialysis patients was 36.47%(62/170). Compared with the good prognosis subgroup, the serum DcR3 and GSDMD levels in the poor prognosis subgroup were increased(t/P=7.598/<0.001, 7.287/<0.001). Age, long dialysis age, high intact parathyroid hormone, high DcR3, and high GSDMD were independent risk factors for poor prognosis in uremic hemodialysis patients[OR(95%CI)=1.120(1.042-1.205),1.061(1.027-1.096),1.002(1.001-1.003),5.349(2.499-11.447),1.894(1.419-2.527)]. The area under the curve of serum DcR3, GSDMD levels and the combination of the two in evaluating the poor prognosis of uremic hemodialysis patients were 0.783,0.789 and 0.871, respectively. The AUC of the combination of the two was greater than that of serum DcR3 and GSDMD levels alone(Z/P=3.492/0.001, 2.997/0.003).ConclusionThe levels of serum DcR3 and GSDMD in uremic hemodialysis patients are increased, which is closely related to the poor prognosis. The combined evaluation of serum DcR3 and GSDMD levels is of high value in evaluating the poor prognosis of uremic hemodialysis patients.

ObjectiveTo investigate the alterations and clinical significance of interleukin-8(IL-8) and CXC chemokine receptor 2(CXCR2) levels in peripheral blood CD34+cells of patients with myelodysplastic syndrome(MDS).MethodsClinical data from 100 MDS patients admitted to the Department of Hematology, People's Hospital of Xinjiang Uygur Autonomous Region, from January 2021 to February 2024 were retrospectively analyzed. Patients were grouped according to the Revised International Prognostic Scoring System(IPSS-R): the low-risk MDS group(n=50) included very low-, low-, and intermediate-risk patients, while the high-risk MDS group(n=50) included high-and very high-risk patients, and 20 healthy subjects were selected as healthy control group.Reverse transcription-polymerase chain reaction(RT-PCR) was used to measure IL-8 and CXCR2 mRNA expression in peripheral blood CD34+cells, while Western blot(WB) was employed to determine IL-8 and CXCR2 protein levels. Differences in IL-8 and CXCR2 expression among groups were compared. Pearson correlation analysis was performed to assess the relationship between IL-8 and CXCR2 in MDS patients. Receiver operating characteristic(ROC) curve analysis was conducted to evaluate the diagnostic value of IL-8 and CXCR2 mRNA in peripheral blood CD34+cells for MDS.ResultsIL-8 and CXCR2 mRNA expression levels followed the order: high-risk MDS group > low-risk MDS group > healthy control group(F/P=351.477/<0.001, 872.932/<0.001). IL-8 and CXCR2 protein levels in peripheral blood CD34+cells followed the same trend: high-risk MDS group > low-risk MDS group > healthy control group(F/P=278.583/<0.001, 455.498/<0.001). Pearson correlation analysis revealed a positive correlation between IL-8 and CXCR2 in peripheral blood CD34+cells of MDS patients(r/P=0.419/0.023). The area under the curve(AUC) for diagnosing MDS using IL-8 mRNA, CXCR2 mRNA, and their combination was 0.754, 0.801, and 0.842, respectively. The combined detection outperformed individual assessments(Z/P=2.345/0.019, 2.750/0.006).ConclusionThe expression levels of IL-8 and CXCR2 in peripheral blood CD34+cells of MDS patients were significantly higher than those in healthy controls and correlated with prognostic risk stratification. Combined detection of IL-8 and CXCR2 mRNA may serve as a potential biomarker for the diagnosis and prognostic assessment of MDS.