ObjectiveTo investigate the expression and clinical significance of serum Omentin-1 and chondroitin sulfate 846(CS846) in elderly patients with rheumatoid arthritis(RA).MethodsA total of 100 elderly RA patients admitted to the Department of Geriatric Medicine of Dongfeng General Hospital, Hubei University of Medicine from April 2022 to April 2024 were selected as the study objects(RA group). Patients with RA were evaluated according to a 28-point joint disease activity score(DAS28) and classified into a low activity subgroup(n=43, DAS28 < 5.0) and a high activity subgroup(n=57, DAS28≥5.0). Another 50 healthy subjects who underwent physical examination in hospital during the same period were selected as healthy control group. The levels of Omentin-1 and CS846 in serum were detected by ELISA. The correlations of serum Omentin-1 and CS846 levels with rheumatoid factor(RF), interleukin(IL)-6, tumor necrosis factor(TNF-α) and DAS28 scores were analyzed by Pearson method. ROC curve to evaluate the diagnostic value of serum Omentin-1 and CS846 levels in RA disease.ResultsOmentin-1 level in RA group was lower than that in healthy control group(t/P=9.583/<0.001), and CS846 level was significantly higher than that in healthy control group(t/P=5.556/<0.001). The levels of RF, IL-6 and TNF-α in RA group were higher than those in healthy control group(t/P=14.413/<0.001,11.708/<0.001,12.036/<0.001).The serum Omentin-1 level of RA patients in high activity subgroup was lower than that in low activity subgroup(t/P=4.914/< 0.001), and the serum CS846 level was significantly higher than that in low activity subgroup(t/P=5.272/<0.001). Serum Omentin-1 expression was negatively correlated with RF, IL-6, TNF-α and DAS28 scores(r=-0.518,-0.435,-0.486,-0.434, all P<0.001). Serum CS846 expression was positively correlated with RF, IL-6, TNF-αand DAS28 scores(r=0.473, 0.496, 0.454, 0.541, all P<0.001). The AUC of serum Omentin-1, CS846 and the combination of OMentin-1 and CS846 in the diagnosis of RA were 0.882, 0.755 and 0.924, respectively, and the efficacy of the combined diagnosis was better than that of single diagnosis(Z/P=2.447/0.014, 4.429/< 0.001).ConclusionSerum Omentin-1 expression is decreased and CS846 expression is increased in elderly RA patients. The combined detection of the two can help diagnose RA.

ObjectiveTo explore the predictive value of serum Nesfatin-1, Kisspeptin, and fatty acid binding protein-4(FABP4) for poor pregnancy outcomes in patients with gestational diabetes.MethodsFrom January 2023 to September 2024, 139 patients with gestational diabetes admitted to the Obstetrical Department of Tangshan Maternal and Child Health Hospital were labeled as study group. Another 139 healthy pregnant women who had normal pregnancy tests were labeled as the healthy control group. Complying with blood glucose control status, the study group was assigned into well controlled group of 49 cases, poorly controlled group of 54 cases, and high-risk group of 36 cases. The study group was assigned into good pregnancy outcome group(n=94) and poor pregnancy outcome group(n=45) based on whether the patient experienced poor pregnancy outcomes. Enzyme linked immunosorbent assay was performed to detect serum Nesfatin-1, Kisspeptin, and FABP4. Logistic regression was used to analyze the influencing factors of poor pregnancy outcomes. ROC curve was drawn to analyze the efficacy of each factor alone and jointly in predicting pregnancy outcome of gestational diabetes.ResultsThe study group had manifestly higher serum Nesfatin-1, Kisspeptin, and FABP4 than healthy control group(P<0.05). The Nesfatin-1, Kisspeptin, and FABP4 in serum increased sequentially in well controlled group, poorly controlled group, and high-risk group(P<0.05). Univariate analysis showed that the proportion of adverse pregnancy history, fasting blood glucose, serum Nesfatin-1, Kisspeptin, and FABP4 levels in the group with poor pregnancy outcomes were higher than those in the group with good pregnancy outcomes(P<0.05). Multivariate logistic regression revealed that elevated levels of serum Nesfatin-1, Kisspeptin and FABP4 were risk factors for poor pregnancy outcome in gestational diabetes(P<0.05). ROC curve revealed that the area under the curve(AUC) of serum Nesfatin-1, Kisspeptin, FABP4 and their joint in predicting poor pregnancy outcomes in patients with gestational diabetes was 0.825, 0.820, 0.838 and 0.939, respectively. The AUC of joint prediction was higher than that predicted by Nesfatin-1, Kisspeptin, and FABP4 alone(Z=4.618, 4.449, 3.925, P<0.05).ConclusionThe serum Nesfatin-1, Kisspeptin and FABP4 in patients with gestational diabetes are elevated, which can be used as potential indicators to predict the poor pregnancy outcome of patients. And the joint prediction of the three is more effective.

ObjectiveTo investigate the roe of Yixinyin in alleviating myocardial ischemia-reperfusion injury(MIRI) and elucidate its potential molecular mechanisms using a network pharmacology approach.MethodsThe active components of Yixinyin and their target genes were identified through the TCMSP and BATMAN databases. MIRI-related target genes were collected from the GeneCards, OMIM, and HPO databases. Intersection analysis was performed to construct a "Yixinyin-active component-MIRI common target" network. Subsequently, a protein-protein interaction(PPI) network was developed using the STRING database and visualized with Cytoscape to identify the core target genes, followed by GO and KEGG enrichment analyses. Molecular docking was conducted between the core target genes and the key active components, with cellular model validation performed.ResultsA total of 146 common target genes for Yixinyin and MIRI were identified, with 18 core target genes further confirmed. GO and KEGG enrichment analyses revealed that these targets are mainly involved in oxidative stress and inflammation-related pathways. Molecular docking indicated the strong binding affinity between the active components of Yixinyin and the core target genes. Cell experiments have shown that Yixinyin inhibits cardiomyocyte apoptosis under ischemic and hypoxic conditions.ConclusionYixinyin may alleviate MIRI through a synergistic effect on multiple targets and pathways.

ObjectiveTo investigate the impacts of transcatheter left gastric artery embolization(LGAE) on glucose and lipid metabolisms and insulin resistance in obese rabbits with type 2 diabetes mellitus(T2DM).MethodsThe experimental rabbits were assigned into a control group, a non-embolization group, and an LGAE group randomly, with 10 rabbits in each group. A high-fat and high glucose diet combined with intraperitoneal injection of streptozotocin(STZ) was used to establish an experimental rabbit model of obesity with T2DM. After femoral artery catheterization, LGAE was guided by percutaneous catheterization. The fasting body mass, fasting blood glucose(FPG) and fasting insulin(FINS) levels of experimental rabbits in each group was measured and recorded before surgery and at 4th, 8th, and 12th weeks after surgery, HOMA-IR and HOMA-β were calculated. The levels of serum lipid[(total cholesterol(TC), triglyceride(TG), high density lipoprotein cholesterol(HDL-C), low density lipoprotein cholesterol(LDL-C)]and the Ghrelin and Leptin were detected by the kit.ResultsAt postoperative 4th, 8th, and 12th weeks, compared with the control group, fasting body mass, FPG, FINS, HOMA-IR, TC, TG, LDL-C, ghrelin and leptin levels were increased in the non-embolization group and the LGAE group, the levels of HOMA-β and HDL-C were decreased(P<0.001). At the 4th, 8th and 12th week after surgery, compared with the non-embolization group, the levels of body weight, FPG, FINS, HOMA-IR, TC, TG, LDL-C, ghrelin and leptin in the LGAE group were decreased, while the levels of HOMA-βand HDL-C were increased(4th week: t/P=9.665/<0.001, 38.240/<0.001, 39.546/<0.001, 92.674/<0.001, 34.687/<0.001, 32.193/<0.001, 42.321/<0.001, 32.194/<0.001, 39.623/<0.001, 14.770/<0.001, 23.362/<0.001; 8th week: t/P=12.490/<0.001, 39.513/<0.001, 36.943/<0.001, 106.661/<0.001, 30.934/<0.001, 24.736/<0.001, 39.583/<0.001, 31.694/<0.001, 34. 430/<0.001, 13.430/<0.001, 24.794/<0.001; 12th week: t/P=15.381/<0.001, 37.054/<0.001, 35.575/<0.001, 113.798/<0.001, 29.579/<0.001, 18.933/<0.001, 36.352/<0.001, 31.680/<0.001, 24. 839/<0.001, 10.329/<0.001, 20.092/<0.001).ConclusionLGAE guided by percutaneous catheterization can reduce blood glucose, alleviate insulin resistance, and improve lipid metabolism in obese experimental rabbits with T2DM.

To report the clinical data of two pediatric patients with acute renal infarction and to conduct a literature review.