Expression level and clinical significance of miＲ-34a-5p and miＲ-149-5p in joint fluid of patients with knee osteoar-
thritis Wang Hao，Tu Feng，Zhao Wenbin，Zhang Lin． Department of Orthopedics，Wuhan First Hospital，Hubei Province，
Wuhan 430022，China
Corresponding author: Wang Hao，E-mail: 506352714@ qq． com
Funding program: General Project of Hubei Natural Science Foundation ( 2018cfc832)
【Abstract】 Objective To analyze the expression and clinical significance of miＲ-34a-5p and miＲ-149-5p in joint
fluid of patients with knee osteoarthritis ( KOA) ． Methods The clinical data of 90 KOA patients from the Orthopedics De-
partment of Wuhan First Hospital from January 2020 to June 2022 were collected． According to the Kellgren-Lawrence ( KL)
standard grading of X-ray film，45 patients were divided into mild KOA group ( grade 1-2 patients) and severe KOA group
( grade 3-4 patients) ． In addition，45 patients with physical examination in the same period were selected as the control
group． The expression of miＲ-34a-5p and miＲ-149-5p in joint fluid of each group was detected by qＲT-PCＲ，and the amount
of expression IL-1β in joint fluid of each group was detected by ELISA． Spearman analyzed the correlation between expression
level of miＲ-34a-5p，miＲ-149-5p and IL-1β in joint fluid，KL grading． The diagnostic value of the expression of miＲ-34a-5p
and miＲ-149-5p in the joint fluid for the severity of KOA was analyzed by the working characteristic curve ( ＲOC) of subjects．
Ｒesults MiＲ-34a-5p，IL-1 in knee joint fluid β Comparison of expression level，severe KOA group ＞ mild KOA group ＞ con-
trol group ( F / P = 19． 21 / ＜ 0． 001，14． 86 / ＜ 0． 001) ． Compared with the expression level of miＲ-149-5p in knee joint fluid，
severe KOA group ＜ mild KOA group ＜ control group ( F / P = 63． 81 / ＜ 0． 001) ． Spearman correlation analysis showed that the
expression level of miＲ-34a-5p and IL-1 β，KL grading showed significant positive correlation ( r / P = 0． 835 / ＜ 0． 001，0．
·113·疑难病杂志 2023 年 3 月第 22 卷第 3 期 Chin J Diffic and Compl Cas，March 2023，Vol． 22，No． 3

835 / ＜ 0． 001) ，and the expression level of miＲ-149-5p was correlated with IL-1 β，KL grading showed significant negative
correlation ( r / P = － 0． 828 / ＜ 0． 001，－ 0． 943 / ＜ 0． 001) ． The ＲOC curve analysis showed that the area under the curve of
miＲ-34a-5p，miＲ-149-5p and their combined prediction of KOA severity were 0． 902，0． 850 and 0． 976，respectively． The
value of their combined prediction of KOA severity was higher than the single index ( Z / P = 240． 70 /0． 046224． 13 /0． 005) ．
Conclusion The expression of miＲ-34a-5p and miＲ-149-5p in the joint fluid of KOA patients is abnormal，and is significant-
ly related to the clinical grade and laboratory indicators of KOA，which can be used as a potential marker for diagnosis and mo-
nitoring of KOA disease activity．
【Key words】 Knee osteoarthritis; miＲ-34a-5p; miＲ-149-5p; Synovial fluid