Analysis of the correlation between the expression of miＲ-21，miＲ-155 and clostridium difficile infection in patients
with ulcerative colitis Pang Guojin，Zhou Meijia，Pi Lijuan，Han Leilei． Department of Emergency Medicine，The First Af-
filiated Hospital of Tsinghua University，Beijing 100016，China
Correspondent author: Han Leilei，E-mail: 176615367@ qq． com
Funding program: Scientific Ｒesearch Program of Beijing Municipal Commission of Science and Technology
( D204700000532006)
【Abstract】 Objective To explore the correlation between the expression levels of serum microＲNA-21 ( miＲ-21) and
miＲ-155 in patients with ulcerative colitis ( UC) and Clostridium difficile infection ( CDI) ． Methods One hundred and twen-
ty-four patients with UC admitted to the First Affiliated Hospital of Tsinghua University from February 2021 to August 2022
were selected as the study subjects． According to whether the UC patients were infected with Clostridium difficile，they were
divided into non-CDI group ( 58 cases) and combined CDI group ( 66 cases) ． At the same time，55 healthy persons were se-
lected as the healthy control group． Collect clinical data of all subjects; Hemoglobin ( Hb) ，albumin ( Alb) ，white blood cell
count ( WBC) ，neutrophil ratio ( NEUT) ，C-reactive protein ( CＲP) ，erythrocyte sedimentation rate ( ESＲ) and other bio-
·492· 疑难病杂志 2023 年 3 月第 22 卷第 3 期 Chin J Diffic and Compl Cas，March 2023，Vol． 22，No． 3

chemical indicators were detected; The expression levels of miＲ-21 and miＲ-155 in serum were detected by real-time fluores-
cence quantitative PCＲ; Pearson＇s method was used to analyze the correlation between the expression levels of serum miＲ-21
and miＲ-155 and biochemical indicators in patients with UC complicated with CDI． Multifactor logistic regression analysis was
used to analyze the factors affecting the occurrence of UC complicated with CDI． The diagnostic value of serum miＲ-21 and
miＲ-155 in patients with UC complicated with CDI was evaluated by the working characteristic curve ( ＲOC) of subjects． Ｒe-
sults Comparison of Hb and Alb levels，combined CDI group ＜ non-CDI group ＜ healthy control group ( F / P = 55． 339 / ＜
0． 001，433． 328 / ＜ 0． 001) ，and comparison of NEUT and CＲP levels，combined CDI group ＞ non-CDI group ＞ healthy con-
trol group ( F / P = 64． 375 / ＜ 0． 001，532． 313 / ＜ 0． 001) ． The levels of WBC and CＲP in CDI group were higher than those
in non-CDI group and healthy control group ( P ＜ 0． 05) ． Compared with the expression level of miＲ-21 and miＲ-155 in ser-
um，the expression level of combined CDI group ＞ non-CDI group ＜ healthy control group ( F / P = 207． 397 / ＜ 0． 001，339．
643 / ＜ 0． 001) ． In patients with UC complicated with CDI，serum miＲ-21 was positively correlated with the expression level
of miＲ-155 ( r / P = 0． 449 / ＜ 0． 001) ，and serum miＲ-21 and miＲ-155 were positively correlated with WBC，NEUT，CＲP
and ESＲ ( miＲ-21: r / P = 0． 257 /0． 037，0． 490 / ＜ 0． 001，0． 405 /0． 001，0． 287 /0． 020; miＲ-155: r / P = 0． 313 /0． 011，0．
526 / ＜ 0． 001，0． 492 / ＜ 0． 001，0． 310 /0． 011) Alb was negatively correlated ( miＲ-21: r / P = － 0． 451 / ＜ 0． 001，－ 0． 417 /
＜ 0． 001． miＲ-155: r / P = － 0． 436 / ＜ 0． 001，－ 0． 397 / ＜ 0． 001) ． High Hb and high Alb are protective factors for UC com-
bined with CDI ［OＲ ( 95% CI) = 0． 527 ( 0． 349 － 0． 767) ，0． 624 ( 0． 440 － 0． 885) ］，and high NEUT，CＲP，miＲ-21 and
miＲ-155 are risk factors for UC combined with CDI ［OＲ ( 95% CI) = 2． 042 ( 1． 207 － 3． 455) ，4． 620 ( 1． 086 － 19． 659) ，
3． 457 ( 1． 625 － 7． 352) ，4． 527 ( 1． 791 － 11． 440) ］． The area under curve ( AUC) of serum miＲ-21，miＲ-155 and their
combined diagnosis of UC with CDI were 0． 915，0． 878 and 0． 950，respectively; The AUC of the combined diagnosis was
higher than that of the single index diagnosis ( Z / P = 2． 565 /0． 010，3． 338 /0． 001) ． Conclusion The expression of miＲ-21
and miＲ-155 in the serum of patients with UC complicated with CDI are both increased，which are risk factors for the occur-
rence of UC complicated with CDI． Combined detection can be used to diagnose the occurrence of UC complicated with CDI．
【Key words】 Ulcerative colitis; Clostridium difficile infection; MicroＲNA-21; MicroＲNA-155; Correlation