Objective To study the expression of serum semaphorin3a(Sema3A), long non coding RNA colon cancer associated transcript 2(CCAT2) and their clinical significance in patients with primary laryngeal cancer(PLC), and to analyze the prognostic value. Methods From February 2019 to February 2021, 104 patients with PLC who underwent surgical treatment(partial or total laryngectomy) were admitted to the department of otolaryngology and head and neck surgery at the Affiliated Hospital of North Sichuan Medical College. The expression of Sema3A mRNA, CCAT2 in tissues was detected by qPCR. Kaplan Meier curve and Cox regression were used to analyze the prognostic factors. Results The levels of Sema3A mRNA and CCAT2 in cancer tissues were higher than those in adjacent tissues, and the difference was statistically significant( t=38.470, 35.015, P<0.001). The expression of Sema3A mRNA in PLC cancer tissue was significantly positively correlated with CCAT2( r=0.678, P<0.001). The levels of Sema3A mRNA and CCAT2 in PLC patients with TNM stage Ⅲ-Ⅳ, lymph node metastasis were higher( t=8.986,10.526,11.195,5.225,P<0.001). The area under the curve(95% CI) of the combination of Sema3A mRNA and CCAT2 for the prognosis of PLC patients was 0.905(0.880-0.948), which was higher than that of Sema3A mRNA [0.844(0.809-0.887)] and CCAT2 [0.815(0.781-0.852)], the difference was statistically significant( Z=4.641, 4.910, P<0.001). The 3-year overall survival rate in the high expression group of Sema3A was 63.42%(26/41), which was lower than 95.24%(60/63) in the low expression group(log rank χ2=10.680, P=0.001); The 3-year overall survival rate in the high expression group of CCAT2 was 67.50%(27/40), which was lower than 92.19%(59/64) in the low expression group(log rank χ2=11.870, P<0.001). TNM stage, lymph node metastasis, Sema3A mRNA and CCAT2 were the factors affecting the prognosis of PLC[OR(95%CI)=1.458(1.106-1.922),1.433(1.128-1.821),1.377(1.125-1.685), 1.315(1.105-1.565)]. Conclusion Elevated levels of Sema3A mRNA and CCAT2 in PLC are associated with adverse clinical pathological features and poor prognosis of PLC patients.

Objective To investigate the effect of astaxanthin(AST) intervention on the pathological process of mice with non-alcoholic fatty liver disease(NAFLD) and its molecular mechanism.Methods This study was conducted from June to November 2023 at the Animal Center of Xinjiang Medical University. A NAFLD mouse model was established using a high-fat diet, and mice were randomly divided into four groups: Control group, NAFLD group, NAFLD+Erastin group and NAFLD+AST group, Immunohistochemistry was used to detect the protein expression of SLC7A11 in liver tissues. Hematoxylin-eosin(HE) staining was performed to observe inflammatory responses and structural changes in liver tissues. Enzyme-linked immunosorbent assay(ELISA) was conducted to measure the levels of blood lipids and inflammatory factors. Additionally, quantitative real-time polymerase chain reaction(RT-qPCR) and Western blot analyses were used to assess the expression levels of ferroptosis-related molecules(GPX4, HMOX1, SLC7A11) and autophagy-related molecules(LC3, p62). Results Immunohistochemistry showed that, compared with the blank control group, the expression of SLC7A11 protein in the NAFLD group decreased(P<0.05). Compared with the NAFLD group, the expression of SLC7A11 protein in the NAFLD+Erastin group decreased(P<0.05), while the expression of SLC7A11 protein in the NAFLD+AST group increased(P<0.05). Compared with the NAFLD+AST group, the expression level of SLC7A11 protein in the NAFLD+Erastin group was lower(P<0.05).The results of HE staining indicated that, compared with the blank control group, the inflammatory response in the liver tissue of the NAFLD group was exacerbated. When compared with the NAFLD group, the inflammatory response in the NAFLD+Erastin group was further aggravated, while that in the NAFLD+AST group was alleviated. AST can effectively reduce the inflammatory response in the liver tissue. The inflammatory response in the NAFLD+AST group was significantly milder than that in the NAFLD+Erastin group. The detection of serum indicators showed that, compared with the blank control group, the levels of triglyceride(TG), total cholesterol(TC), and low-density lipoprotein Cholesterol(LDL-C) in the serum of mice in the NAFLD group were significantly increased(P<0.05). Compared with the NAFLD group, the levels of TG, TC, and LDL-C in the NAFLD+Erastin group were all increased(P<0.05), while the levels of TG, TC, and LDL-C in the NAFLD+AST group were all decreased(P<0.05). The levels of TG, TC, and LDL-C in the NAFLD+Erastin group were significantly higher than those in the NAFLD+AST group(P<0.05).Compared with the blank control group, the level of glutathione(GSH) in the NAFLD group was decreased(P<0.05), while the levels of reactive oxygen species(ROS), malondialdehyde(MDA), tumor necrosis factor-α(TNF-α), and interleukin-6(IL-6) were increased(P<0.05).Compared with the NAFLD group, the level of GSH in the NAFLD+AST group was increased(P<0.05), and the levels of ROS, MDA, TNF-α, and IL-6 were decreased(P<0.05). In the NAFLD+Erastin group, except that the level of GSH was decreased(P<0.05), the levels of ROS, MDA, TNF-α, and IL-6 were all increased(P<0.05).Compared with the NAFLD+Erastin group, the level of GSH in the NAFLD+AST group was significantly increased(P<0.05), and the levels of ROS, MDA, TNF-α, and IL-6 were decreased to some extent(P<0.05).Molecular-level analysis revealed that qRT-PCR results showed that, compared with the blank control group, the expressions of GPX4, HMOX1, p62, and SLC7A11 in the NAFLD
group were downregulated(P<0.05), while the expression of LC3B was upregulated(P<0.05).Compared with the NAFLD group, the expressions of GPX4, HMOX1, SLC7A11, and p62 in the NAFLD+Erastin group were all decreased(P<0.05), and the level of LC3B was increased(P<0.05). In the NAFLD+AST group, the level of LC3B was decreased(P<0.05), and the expressions of GPX4, HMOX1, SLC7A11, and p62 were all increased(P<0.05).The expression levels of GPX4, HMOX1, SLC7A11, and p62 in the NAFLD+AST group were higher than those in the NAFLD+Erastin group(P<0.05), while the expression of LC3B was decreased(P<0.05).The results of Western Blot showed that compared with the blank control group, the expression of LC3B in the NAFLD group was upregulated(P<0.05), and the protein expressions of GPX4, HMOX1, p62, and SLC7A11 were all downregulated(P<0.05).Compared with the NAFLD group, the expression of LC3B in the NAFLD+Erastin group was upregulated(P<0.05), and the expressions of GPX4, HMOX1, SLC7A11, and p62 were downregulated(P<0.05). In the NAFLD+AST group, the expression of LC3B was downregulated(P<0.05), and the expressions of GPX4, HMOX1, SLC7A11, and p62 were all upregulated(P<0.05).The expression of LC3B in the NAFLD+Erastin group was lower than that in the NAFLD+AST group(P<0.05), and the expressions of GPX4, HMOX1, SLC7A11, and p62 were all increased(P<0.05).Conclusion AST intervention may regulate the ferroptosis and autophagy pathways by upregulating the expression of SLC7A11, thereby improving the pathological changes of liver tissues in NAFLD mice, reducing the levels of oxidative stress and inflammatory response, and providing potential new targets and innovative ideas for the treatment of NAFLD.

Objective To systematically evaluate the clinical efficacy and safety of Yiqi Huoxue therapy combined with conventional western medicine in the treatment of heart failure in the elderly through systematic review and Meta-analysis. Methods The original clinical literature related to the treatment of senile heart failure with Yiqi Huoxue therapy combined with conventional western medicine was searched by computer. The database was selected from commonly used Chinese and English databases, including China National Knowledge Infrastructure(CNKI), China Biology Medicine Disc(CBM), Wanfang Literature Data platform, VIP journal database platform. English databases included PubMed, Embase and The CochraneLibrary. The literature retrieval period was from January 1, 2015 to December 1, 2020. The latest version of Revman 5.4 software was used to conduct statistical meta-analysis of the relevant data of the included studies. Results A total of 21 RCTS were included, involving 2070 patients(1033 in the control group and 1037 in the experimental group). The results of Meta-analysis showed that The efficacy of cardiac function in the experimental group was significantly higher than that in the control group, and the difference was statistically significant[MD=4.11, 95%CI(2.90, 5.82), P<0.00001]. LVEF in the experimental group was significantly higher than that in the control group[MD=5.41, 95%CI(4.18, 6.64), P<0.01], the 6MWD of the experimental group was significantly higher than that of the control group [MD=64.61, 95%CI(49.39, 79.83), P<0.01]. NT-proBNP in the experimental group was significantly lower than that in the control group, and the difference was statistically significant[MD=-119.07, 95%CI(-148.56,-89.58); LVEDD in the experimental group was significantly lower than that in the control group, the difference was statistically significant [MD=-2.41,95%CI(-4.43,-0.39), P<0.01]. Conclusion Yiqi Huoxue therapy combined with conventional western medicine is safe and effective in the treatment of heart failure in the elderly. However, due to the small number of included studies and the general quality of the studies, high-quality, large-sample, randomized, double-blind trials are needed to confirm it in the future.

Report one case of congenital hepatic fibrosis with Caroli patients clinical data, and carry on the literature review.

Report the clinical data of a case with COPA syndrome and conduct literature review.