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:Objective To explore the predictive value of waist to height ratio(WHTR), glycated hemoglobin(HbA1c), high-density lipoprotein cholesterol(HDL-C), systolic blood pressure(SBP), and diastolic blood pressure(DBP) indicators for the prognosis of patients with preserved ejection fraction heart failure(HFpEF). Methods A retrospective study was conducted on 183 patients with HFpEF who visited the Emergency General Hospital from February 2021 to February 2022. All patients were followed up for one year after standardized treatment. The patients were divided into MACE group(49 cases) and No MACE group(134 cases) based on their readmission due to major adverse cardiovascular events(MACE) within one year. The clinical data of the two groups were compared. Multivariate logistic regression was used to analyze the factors affecting the poor prognosis of HFpEF patients, and receiver operating characteristic(ROC) curves were drawn to analyze the predictive value of WHTR, HbA1c, HDL-C, SBP, and DBP in evaluating poor prognosis at admission. Results The incidence of MACE in 183 patients during the 1-year follow-up period was 26.78%(49/183). Compared with the No MACE group, the MACE group had significantly higher HbA1c, SBP, DBP, BMI, WC, troponin I, NT proBNP, NYHA functional class Ⅲ+Ⅳ ratio, systemic inflammatory index(SII), and neutrophil to lymphocyte ratio(NLR), while WHTR, HDL-C, and left ventricular ejection fraction(LVEF) were lower than those in the No MACE group, and the differences were statistically significant( P<0.05); Through multiple logistic regression analysis, the results showed that high WHTR and HDL-C were independent protective factors affecting poor prognosis in HFpEF patients [OR(95%CI)=0.874(0.786-0.971), 0.855(0.757-0.965), all P<0.05], while high SII, NLR, and NYHA functional class Ⅲ+Ⅳ were independent risk factors [OR(95%CI)=1.143(1.013-1.291), 1.134(1.024-1.256), 2.784(1.121-6.913), all P<0.05]. The ROC curve shows that the area under the curve of WHTR, HbA1c, HDL-C, SBP, and DBP predicting poor prognosis is 0.845, 0.732, 0.785, 0.669, and 0.683, respectively, indicating good predictive value. Conclusion WHTR, HDL-C, NYHA functional classification, SII, and NLR can affect the readmission of HFpEF patients after treatment with MACE, leading to a higher incidence of adverse prognosis. WHTR, HbA1c, HDL-C, SBP, and DBP have important predictive value for their adverse prognosis.

Objective To investigate the relationship between transvaginal ultrasonography parameters, serum vascular endothelial growth factor(VEGF), hypoxia-inducible factor-1α(HIF-1α) and endometrial receptivity in patients with polycystic ovary syndrome(PCOS).Methods From April 2019 to December 2021, the Department of Gynecology of the First Affiliated Hospital of Heilongjiang University of Traditional Chinese Medicine diagnosed and treated 120 PCOS patients. According to the results of endometrial pinocytosis, 42 patients were divided into good receptive group and 78 patients into poor receptive group. Compare the general data, transvaginal ultrasound parameters and serum VEGF, HIF-1 between the two groups α Level, Pearson method to analyze ultrasonic parameters and serum VEGF, HIF-1 α. Logistic regression analysis was used to analyze the risk factors of endometrial receptivity impairment. Results There was no significant difference in age, body mass and body mass index(BMI) between the two groups( P>0.05). The proportion of patients with hyperandrogenism and hyperinsulinemia in the poor receptive group was higher than that in the good receptive group( χ2/P=12.897/<0.001、5.500/0.019). The mean number of pinocytotic processes, endometrial thickness, endometrial volume and serum VEGF, HIF-1α in patients with poor receptivity were lower than those in the well tolerated group( t=13.804, 9.021, 15.004, 11.453, 11.671,P<0.001), and the resistance index(RI) and pulsatility index(PI) of uterine spiral artery were higher than those in the well tolerated group( t=4.198,8.008,P<0.001). Endometrial pinocytosis, endometrial thickness, endometrial volume and serum VEGF, HIF-1α in patients with PCOS were positive correlation(VEGF: r/P=0.364/0.007, 0.562/<0.001, 0.482/0.011; HIF-1 α: r/P=0.405/0.014, 0.409/0.017, 0.597/<0.001), RI and PI of uterus, serum VEGF and HIF-1α were negative correlation(VEGF: r/P=-0.631/0.002,-0.593/0.007; HIF-1 α: r/P=-0.689/0.001、-0.571/0.009). Hyperandrogenism, hyperinsulinemia, low endometrial thickness, low endometrial volume, high RI, high PI, low serum VEGF and HIF-1 α were risk factors for poor endometrial receptivity in PCOS patients [OR(95%CI)=1.361(1.115-1.607)、1.294(1.017-1.571)、1.852(1.324-2.380)、2.586(1.714-3.458)、1.763(1.203-2.320)、1.608(1.182-2.034)、1.526(1.056-1.996)、1.267(0.571-1.963)].Conclusion Transvaginal ultrasound parameters and serum VEGF, HIF-1α in patients with PCOS is related and is a risk factor for patients with poor endometrial receptivity, which can effectively evaluate patients' endometrial receptivity.

Objective To investigate the relationship between serum human chorionic gonadotropin(β-HCG), placental growth factor(PLGF) and pregnancy outcomes in patients with gestational hypothyroidism. Methods From May 2023 to May 2024, 102 patients with hypothyroidism during pregnancy in our hospital were selected to form the hypothyroidism group. At the same time, 102 healthy pregnant women who underwent pregnancy examination during the same period were considered as control group. Patients with hypothyroidism were assigned into the adverse group and the good group based on pregnancy outcomes. Chemiluminescence immunoassay was used to detect serum β-HCG. ELISA was used to detect serum PLGF. Multivariate Logistic regression was used to analyze the influencing factors of adverse pregnancy outcomes. ROC curve was used to evaluate the predictive value of serum β-HCG and PLGF for adverse pregnancy outcomes. Results For the control group, the hypothyroidism group had higher serum β-HCG and lower serum PLGF( t/P=18.208/<0.001, 31.703/<0.001). For the control group, the hypothyroidism group had a higher overall incidence of adverse pregnancy outcomes( χ2/P=4.293/0.038). The adverse group had higher thyroid stimulating hormone and β-HCG, and lower PLGF than good group( t/P=4.940/<0.001, 7.015/<0.001, 5.260/<0.001). Multivariate Logistic regression analysis showed that high TSH and β-HCG were independent risk factors for adverse pregnancy outcomes in patients with hypothyroidism during pregnancy[OR(95%CI)=5.037(1.909-13.290), 4.456(2.005-8.616)]. High PLGF was an independent protective factor[OR(95%CI)=0.320(0.121-0.781)]. ROC curve showed that the AUC of serum β-HCG, PLGF, and their joint in predicting adverse pregnancy outcomes was 0.776(95%CI: 0.683-0.853), 0.761(95%CI: 0.666-0.840), and 0.880(95%CI: 0.801-0.936), respectively. The AUC predicted by the joint of β-HCG and PLGF was manifestly greater than the AUC predicted by them alone( Z/P=2.224/0.026, Z/P=2.546/0.011). Conclusion Serum β-HCG increases and PLGF decreases in patients with gestational hypothyroidism. Both are influencing factors for adverse pregnancy outcomes, and the joint detection of these two indicators has higher value in predicting adverse pregnancy outcomes.

Objective To investigate the expression of a disintegrin and metalloprotease 10(ADAM10), ubiquitin-conjugating enzyme E2T(UBE2T) in ovarian cancer tissues and their relationship with clinical pathological features and prognosis of patients. Methods From September, 2020 to September, 2023, 78 cases of ovarian cancer were diagnosed by pathology after gynecological treatment in Tongchuan People's Hospital(North Hospital), Shaanxi Province. The relationship between the expression of ADAM10 and UBE2T in ovarian cancer tissues and prognosis was analyzed using Kaplan-Meier method. The factors influencing the prognosis of ovarian cancer patients were analyzed using multiple Logistic regression. Results The positive expression rates of ADAM10 and UBE2T in ovarian cancer tissues were greatly higher than those in adjacent tissues( χ2=12.955, 13.952, P<0.001). The expressions of ADAM10 and UBE2T in ovarian cancer were higher than those in FIGO Ⅲ～Ⅳ, lymph node metastasis and low differentiation, and no lymph node metastasis and moderate/high differentiation( χ2/P=14.857/<0.001, 9.916/0.002, 10.858/0.001, 13.791/<0.001, 16.458/<0.001, 6.334/0.012). The 78 ovarian cancer patients were followed up for 1 year, with a total of 56 survivors and 22 deaths. The overall 1-year survival rate was 71.79%(56/78). The positive expression rate of ADAM10 and UBE2T in ovarian cancer tissue of death subgroup was higher than that of survival subgroup( χ2=13.038, 14.832, P<0.001). There was a statistically significant difference in the proportions of patients in subgroup 2 with FIGO stageⅢ-Ⅳ, lymph node metastasis, and low degree of differentiation(χ2/P=8.104/0.004,7.503/0.006,9.254/0.002). The one-year survival rates of patients with positive expression of ADAM10 and UBE2T in ovarian cancer tissues were 54.76%(23/42) and 54.55%(24/44), respectively. The one-year survival rates of patients with negative expression of ADAM10 and UBE2T were 91.67%(33/36) and 94.12%(32/34), respectively. The one-year survival rates of patients with positive expression of ADAM10 and UBE2T in ovarian cancer tissues were greatly lower than those of patients with negative expression of ADAM10 and UBE2T(Log Rank χ2=13.305, 13.978, P<0.01). FIGO Ⅲ～Ⅳ stage, lymph node metastasis, low degree of differentiation, positive ADAM10 and UBE2T were independent risk factors for the prognosis of ovarian cancer patients[OR(95%CI)=2.537(1.545-1.166), 2.614(1.646-4.151), 1.3 97(1.297-3.001), 2.625(1.481-4.625), 1.674(1.245-2.251)].Conclusion The positive expression of ADAM10 and UBE2T in ovarian cancer tissues is closely related to the clinical pathological features and prognosis of patients.

Objective To investigate the relationship between nucleotide binding oligomerization domain like receptor 5(NLRC5), NOP2/Sun RNA methyltransferase family member 2(NSUN2) in endometrial cancer(EC) and clinical pathological features and prognosis. Methods One hundred and thirty-six EC patients admitted to the Department of Gynecology, Shunyi Maternal and Child Health Hospital of Beijing from February 2016 to February 2019 were selected. Immunohistochemistry and qPCR were used to detect the protein and mRNA expression of NLRC5 and NSUN2 in EC cancer tissues and adjacent tissues. The differential expression of NLRC5 and NSUN2 mRNA in EC patients with different clinical pathological parameters were compared. The value of NLRC5 and NSUN2 mRNA expression in evaluating the prognosis of EC patients analyzed by the receiver operating characteristic curve. Kaplan Meier curves and COX regression analysis were used to investigate the factors affecting the prognosis of EC patients. Results Compared with adjacent tissues, the positivity rates of NLRC5 mRNA and protein were lower in cancer tissues, while the positivity rates of NSUN2 mRNA and protein were higher( t/χ2=14.428,159.835, 31.642,143.753,P<0.001). There were significant differences in NLRC5 and NSUN2 mRNA expression among EC patients with different FIGO stages and lymph node metastasis. Patients with FIGO stage Ⅲ and lymph node metastasis EC had lower NLRC5 mRNA expression and higher NSUN2 mRNA expression in cancer tissues(both P<0.05). The optimal cutoff values for prognostic evaluation of NLRC5 and NSUN2 mRNA expression in EC cancer tissues are 2.52 and 3.83, respectively. The 5-year overall survival(OS) of the NLRC5 mRNA high and low group were 91.67%(22/24) and 73.21%(82/112), respectively, with statistically significant differences in the curves(Log Rank χ2=3.402, P=0.012). The 5-year OS of the NSUN2 mRNA high and low group were 42.31%(11/26) and 84.55%(93/110), respectively, with statistically significant differences(Log Rank χ2=18.910, P<0.001). FIGO stage Ⅲ, lymph node metastasis, high NSUN2 mRNA were risk factors affecting the prognosis of EC patients, while high NLRC5 mRNA was a protective factor[HR(95%CI)=1.449(1.148-1.830), 1.442(1.124-1.850), 1.570(1.145-2.152), 0.611(0.462-0.807)]. Conclusion The decreased expression of NLRC5 and increased expression of NSUN2 in EC are associated with FIGO staging and lymph node metastasis, and are biomarkers for evaluating the prognosis of EC.