Relationship between serum FGF5 , TF levels and adverse pregnancy outcome in pre-eclampsia pregnant womenCuZhangxia, Zhang Na Department of Obstetrics, the Second Afiliated Hospital of Shaanxi Unwersity of Traditional ChineseMedicine, Shaanxi Province , Xianyang 712000  China

Corresponding author: Zhang Na. E-mail: zhyxbi01 @ 163. comFunding program : Special Scientific Research Plan Project of Shaanxi Provincial Department of Education ( 19 JK0224)

Abstract：

Objective To explore the correlation between the levels of serum fibroblast growth factor 5(FGF5), tissue factor(TF) and adverse pregnancy outcome in pre-eclampsia pregnant women. Methods Seventy-two pregnant women with preeclampsia who were examined and delivered in the Second Affiliated Hospital of Shaanxi University of Traditional Chinese Medicine from January 2017 to March 2022 were selected as the observation group. According to the severity of the patient's condition, they were divided into mild subgroup of 40 cases and severe subgroup of 32 cases. According to the pregnancy outcome of preeclampsia patients, they were divided into poor pregnancy outcome subgroup of 22 cases and good pregnancy outcome subgroup of 50 cases. In addition, 78 singleton pregnant women with healthy obstetrics in the same period were selected as the healthy control group. The expression level of serum FGF5 was detected by real-time quantitative PCR, the level of serum TF was determined by enzyme-linked immunosorbent assay, and the level of activated partial prothrombin time(APTT), fibrinogen(FIB), prothrombin time(PT), and platelet(PLT) in plasma were detected by automatic hemagglutination instrument. Pearson method was used to analyze the correlation between serum FGF5, TF and plasma coagulation index in patients with preeclampsia. Logistic regression analysis was used to analyze the independent risk factors affecting the adverse pregnancy outcome of pre-eclampsia pregnant women. Results Compared with the healthy control group, the expression level of serum FGF5 and TF in the observation group increased(t/P=19.916/<0.001, 14.530/<0.001). Compared with mild subgroup, the expression level of serum FGF5 and TF in severe subgroup increased(t/P=5.624/<0.001, 6.938/<0.001). Compared with the subgroup with good pregnancy outcome, the levels of APTT, PT and PLT in the subgroup with poor pregnancy outcome decreased(t/P=12.202/<0.001, 4.294/<0.001, 78.325/<0.001), and the expression levels of FIB, FGF5 and TF increased(t/P=6.436/<0.001, 10.600/<0.001, 12.562/<0.001). Pearson analysis showed that serum FGF5 and TF were negatively correlated with APTT, PT and PLT(FGF5:r=-0.386,-0.416,-0.463; TF:r=-0.394,-0.434,-0.459,P<0.001), and positively correlated with FIB(r=0.447, 0.428, P<0.001). Logistic regression analysis showed that high FGF5, high TF, high FIB and low PLT were independent risk factors for adverse pregnancy outcome in pre-eclampsia pregnant women [OR(95% CI)=2.432(1.577-3.750), 2.203(1.494-3.248), 1.957(1.169-3.277), 2.018(1.327-3.069),P<0.05].Conclusion The expression level of serum FGF5 and TF in pre-eclampsia pregnant women was significantly increased, which was an independent risk factor for adverse pregnancy outcome in pre-eclampsia pregnant women.

Preeclampsia;                    Fibroblast growth factor 5;                    Tissue factor;                    Adverse pregnancy outcomes;                    Correlation;