Objective To investigate the effects of selenium on the proliferation, apoptosis, migration, invasion, malondialdehyde(MDA) and glutathione peroxidase(GSH-Px) activities of lung cancer cells(A549) induced by cisplatin(CIS). Methods From July 2021 to March 2022, the experiment was conducted in the Selenium Laboratory of the Central Hospital of Enshi Tujia and Miao Autonomous Prefecture. The median inhibitory concentration(IC50) of CIS and sodium selenite(Na2SeO3) was calculated on A549 cells in logarithmic phase. A549 cells were divided into control group(Group C), CIS group, Na2SeO3 group, CIS+Na2SeO3 group. Group C was routinely cultured for 48 h, and CIS group was added with 12 μmol/L CIS was cultured for 48 h, Na2SeO3 group was added with Na2SeO3 110 nmol/L for 48 h, CIS+Na2SeO3 group was added with Na2SeO3 110 nmol/L for 24 h, and then 12 μmol/L CIS was cultured for 24 h. CCK-8 method was used to detect the viability of A549 cells, flow cytometry was used to detect the apoptosis of A549 cells, Western blot was used to detect the expression of apoptosis and migration invasion proteins, and Transwell test was used to detect the number of A549 cells migrating and invading; The levels of ROS, MDA, 4-HNE and GSH-Px were measured. Results The IC50 of CIS is 12.02 μmol/L, IC50 of Na2SeO3 is 110.20 nmol/L. Compared with group C, the activity, migration, invasion, MMP-9, MMP-3 protein levels of A549 cells in CIS group and Na2SeO3 group decreased(F/P=147.876/<0.001, 78.926/<0.001, 43.071/<0.001, 36.862/<0.001, 75.431/<0.001), apoptosis rate, cleaved caspase9, cleaved caspase3 protein, ROS, MDA The level of 4-HNE increased significantly(F/P=15.625/<0.001, 8.131/0.004, 22.371/< 0.001, 45.779/< 0.001, 5.216/0.019, 25.084/< 0.001), and there was no significant difference in GSH-Px activity(P>0.05). Compared with CIS group, A549 cells in CIS+Na2SeO3 group had lower activity, migration, invasion, MMP-9, MMP-3 protein levels(t/P=9.340/< 0.001, 9.573/< 0.001, 9.746/<0.001, 6.788/0.001, 9.481/< 0.001), higher apoptosis rate, cleaved caspase9, cleaved caspase3 protein, ROS, MDA, 4-HNE levels(t/P=10.911/< 0.001, 9.098/< 0.001, 10.809/< 0.001, 6.069/0.002, 9.218/< 0.001, 15.945/< 0.001), There was no significant difference in GSH Px activity(P>0.05). Conclusion Na2SeO3 can enhance the apoptosis of A549 cells induced by CIS and inhibit their proliferation, migration and invasion, which may be related to the promotion of ROS and MDA production.

Objective To analyze the value of low-dose spiral CT(LDCT) combined with serum lung cancer autoantibodies in early diagnosis of lung cancer. Methods From August 2020 to August 2022, 120 patients with lung cancer were selected as the lung cancer group in the Department of Respiratory and Critical Care Medicine of Yan'an People's Hospital, and 46 patients with benign lung diseases in the same period were selected as the control group. LDCT scanning was performed on all patients. Seven tumor related autoantibodies [7-TAAB, including p53, protein gene product 9.5(PGP9.5), SRY box transcription factor 2(SOX2), G antigen 7(GAGE7) ATP binding RNA helicase(GBU4-5), melanoma antigen A1(MAGE A1), tumor associated gene(CAGE)] levels. The value of LDCT combined with serum 7-TAAB in the early diagnosis of lung cancer was analyzed by subject operating characteristic curve(ROC). Results The proportion of ground glass nodule, air bronchogram sign, vacuole sign, pleural indentation sign, spiculation sign and lobulation sign in the lung cancer group was higher than that in the control group(χ2/P=5.743/0.017,8.260/0.004,7.275/0.007,11.499/0.001,14.118/<0.001,11.474/0.001).Among 166 patients, 114 cases were diagnosed as lung cancer positive by LDCT, 52 cases were diagnosed as lung cancer negative, 24 cases were misdiagnosed as benign lesions, and 28 cases were misdiagnosed as lung cancer benign lesions. Serum p53, PGP9.5, SOX2, GAGE7, GBU4-5, MAGE A1, CAGE levels in lung cancer group were higher than those in control group(Z=5.168, 4.170, 4.660, 4.561, 4.039, 5.528, 4.981, P<0.001). The positive rates of serum p53, SOX2, GAGE7, MAGE A1, CAGE, 7-TAAB in lung cancer group were higher than those in control group(χ2/P=18.669/<0.001, 9.114/0.005, 13.676/<0.001,12.377/<0.001, 6.794/0.009, 27.041/<0.001). The area under the curve of LDCT, serum 7-TAAB and their combination in diagnosing early lung cancer were 0.704, 0.725 and 0.886, respectively. The diagnostic efficacy of LDCT combined with 7-TAAB was higher than that of single index(Z=4.000, 4.184, P<0.001). Conclusion LDCT combined with serum autoantibody of lung cancer is of high value in early diagnosis of lung cancer.

Objective To analyze the relationship between serum miR-124-3p, miR-361-5p and clinicopathological characteristics, chemosensitivity, and phosphatidylinositol 3 kinase/threonine protein kinase/mammalian rapamycin target protein(PI3K/AKT/mTOR) signaling pathway in patients with advanced gastric cancer.Methods Ninety patients with advanced gastric cancer admitted to the Department of Gastroenterology of the Third People's Hospital of Yunnan Province from March 2020 to February 2022 were selected as the study group, and 45 health examinees from hospitals in the same period were selected as the healthy control group. The expression levels of serum miR-124-3p and miR-361-5p were compared between the two groups, and the correlation between serum miR-124-3p and miR-361-5p and the clinicopathological characteristics of patients with advanced gastric cancer and the levels of PI3K, AKT, mTOR mRNA was analyzed. Results The relative expression levels of serum miR-124-3p and miR-361-5p in the study group were lower than those in the healthy control group(t/P=34.613/<0.001, 31.233/<0.001). The serum miR-124-3p and miR-361-5p levels in patients with poorly differentiated advanced gastric cancer were lower than those in patients with moderately and highly differentiated gastric cancer(P<0.05), and the serum miR-124-3p levels in patients with moderately differentiated gastric cancer were lower than those in patients with highly differentiated gastric cancer(P<0.05), but="" there="" was="" no="" significant="" difference="" between="" the="" serum="" mir-361-5p="" levels="" in="" patients="" with="" moderately="" and="" highly="" differentiated="" gastric="" p="">0.05). The serum miR-124-3p and miR-361-5p levels in TNM stage IV patients were lower than those in stage III B patients(t/P=17.314/<0.001, 20.734/<0.001). 90 patients completed more than 2 cycles of chemotherapy, chemotherapy sensitivity accounted for 37.78%(34/90), chemotherapy resistance accounted for 62.22%(56/90). The relative expression of serum miR-124-3p and miR-361-5p in chemotherapy sensitive subgroup was higher than that in chemotherapy resistant subgroup(t/P=33.766/<0.001, 32.659/<0.001), and the relative expression of PI3K, AKT, mTOR mRNA in chemotherapy sensitive subgroup was lower than that in chemotherapy resistant subgroup(t/P=14.134/<0.001, 15.936/<0.001, 7.104/<0.001). Pearson correlation analysis showed that serum miR-124-3p, miR-361-5p were negatively correlated with the relative expression of PI3K, AKT and mTOR in patients with advanced gastric cancer(miR-124-3p: r/P=-0.315/0.011,-0.402/0.002,-0.554/<0.001; miR-361-5p: r/P=-0.356/0.006,-0.427/<0.001,-0.510/<0.001).Conclusion Serum miR-124-3p and miR-361-5p are related to the differentiation degree, TNM stage and chemotherapy sensitivity of advanced gastric cancer, and may be related to chemotherapy resistance through negative regulation of PI3K/AKT/mTOR signal pathway.

Objective To analyze the expression of SERPINE1 in gastric cancer and its clinical significance.Methods The expression of SERPINE1 in 33 kinds of cancer tissues was investigated by searching the TIMER database. By retrieving the TCGA database, the RNA-Sep data and clinical information of 375 gastric cancer tissues and 32 paracancerous tissues were downloaded, and the gene expression profile chips of 111 gastric cancer tissues and 21 paracancerous tissues were downloaded by retrieving the GSE54129 data set of the GEO database. The TCGA data set and SERPINE1 in the GSE54129 data set were respectively used to analyze the expression differences between cancerous tissues and paracancerous tissues, COX analyzed the impact of clinical baseline data and SERPINE1 mRNA expression level on the prognosis of gastric cancer patients in TCGA data set. Kaplan Meier survival curve and ROC were drawn to analyze the relationship between SERPINE1 mRNA expression and prognosis. Gene enrichment analysis(GSEA) was used to explore and predict SERPINE1 involved pathways.Results TIMER database analysis showed that SERPINE1 was significantly highly expressed in 8 kinds of cancer tissues(P<0.05). The analysis of TCGA data set and GSE54129 data set showed that the expression of SERPINE1 in gastric cancer tissues was higher than that in adjacent tissues(P<0.001). Multivariate COX analysis showed that high levels of pathological Stage4 and SERPINE1 mRNA expression were independent risk factors affecting the prognosis and survival time of gastric cancer patients [HR(95% CI)=3.061(1.107-8.464),1.778(1.224-2.582),P<0.05]. Kaplan-Meier survival curve analysis showed that the total survival time of the high expression group of SERPINE1 was significantly lower than that of the low expression group(P<0.001). ROC curve analysis showed that the area under the curve of SERPINE1 expression predicting 1-, 3-, and 5-year survival probability was 0.598, 0.649, and 0.731, respectively. GESA analysis showed that SERPINE1 was correlated with epithelial mesenchymal transformation, inflammatory reaction, cell apical junction complex, KRAS pathway, angiogenesis and other pathways. Conclusion The expression of SERPINE1 in gastric cancer is obviously up-regulated, and its high expression indicates poor prognosis of gastric cancer patients. SERPINE1 can be used as a biological marker affecting the prognosis of gastric cancer patients, and as a potential therapeutic target.

Objective To observe the effect of immune checkpoint inhibitor combined with ranvartinib in the treatment of transcatheter arterial chemoembolization(TACE) against advanced primary liver cancer(PLC) and its influence on serum platelet derived growth factor(PDGF) and vascular endothelial growth factor(VEGF). Methods From January 2019 to January 2022, 104 patients with PLC in advanced stage of TACE resistance who were admitted to the General Surgery Center of Beijing You'an Hospital Affiliated to Capital Medical University were selected as the research objects. They were divided into control group and observation group according to the random number table, with 52 patients in each group. The control group was treated with ranvartinib, and the observation group was treated with immunosuppressant combined with ranvartinib. To compare the efficacy of the two groups, the levels of tumor markers [alpha fetoprotein(AFP), carcinoembryonic antigen(CEA) α-L-fucosidase(AFU)], the core quality of life scale(QLQ-C30) score, angiogenic factors [PDGF, basic fibroblast growth factor(bFGF), VEGF], and the incidence of adverse reactions. Results The disease control rate in the observation group was higher than that in the control group(75.00% vs 55.77%, χ2/P=4.248/0.039). Compared with that before treatment, after 3 months of treatment, the levels of serum AFP, CEA and AFU in both groups decreased, and the levels in the observation group were lower than those in the control group(t/P=5.399/<0.001, 6.030/<0.001, 3.086/0.003). The scores of QLQ-C30 in both groups decreased, and those in the observation group were lower than those in the control group(t/P=3.060/0.003). The levels of serum PDGF, bFGF and VEGF in the two groups were lower than those in the control group(t/P=7.386/<0.001, 2.513/0.014, 4.796/0.003). The incidence of diarrhea and dermatitis in the observation group was higher than that in the control group(χ2/P=7.121/0.008, 6.310/0.012), but all of them were mild. After rest and symptomatic treatment, the symptoms were alleviated. Conclusion The combination of immunocheckpoint inhibitor and ranvartinib in the treatment of TACE resistant patients with advanced PLC can reduce the levels of serum PDGF and VEGF, improve tumor reactivity, reduce the level of tumor markers, and improve the quality of life, which is a safe and effective treatment.