Objective To analyze the diagnostic value of plasma human cartilage glycoprotein 39(YKL-40), C-reactive protein(CRP), and interleukin-6(IL-6) in coronary artery injury(CAL) in children with Kawasaki disease(KD). Methods 125 children with KD admitted to the Department of Pediatrics of Lanzhou University Second Hospital from April 1, 2018 to December 31, 2021 were selected as the KD group. They were further divided into the CAL subgroup of 53 cases and the NCAL subgroup of 72 cases based on whether they were combined with CAL. In addition, 125 healthy children who were matched in gender and age during the same period were selected as the healthy control(HC) group and 125 children with fever due to digestive tract infection as the fever control(FC) group. Collect clinical data of each group, and detect the plasma levels of YKL-40, CRP, and IL-6 in all tested children. Multivariate logistic regression analysis was used to analyze the risk factors for the occurrence of CAL in children with KD. The value of plasma YKL-40, CRP, and IL-6 in the diagnosis of CAL in children with KD was analyzed by subject performance characteristic curve(ROC).Results Comparison of plasma levels of YKL-40, CRP, and IL-6 showed that KD group>FC group>HC group(F/P=296.352/<0.001, 35.162/<0.001, 20.266/<0.001). The plasma levels of YKL-40, CRP, and IL-6 in the CAL subgroup were higher than those in the NCAL subgroup(t/P=7.434/<0.001, 7.967/<0.001, 7.444/<0.001); The proportion of pretreatment thermal duration, IVIG non response, and IVIG start time ≥ 10 days in CAL subgroup was higher than that in NCAL subgroup(χ2/P=6.831/<0.001,8.304/0.004,3.953/0.047).Multivariate logistic regression analysis showed that non response to IVIG, high YKL-40, high CRP, and high IL-6 were risk factors for developing CAL in children with KD [OR(95%CI)=4.627(2.072 to 10.336), 1.441(1.128 to 1.840), 1.540(1.106 to 2.145), and 1.343(1.098 to 1.644)]. The ROC curve analysis showed that the area under the curve of plasma YKL-40, CRP, IL-6, and their combination in the diagnosis of CAL in children with KD was 0.813, 0.846, 0.821, and 0.923. The combined diagnostic efficacy of the three indicators was higher than that of the single indicator diagnosis(Z/P=2.965/0.012, 2.536/0.020, 2.308/0.025). Conclusion The average increase in plasma YKL-40, CRP, and IL-6 levels in children with KD is related to the occurrence of CAL in children with KD. Combining the three indicators has high value in the diagnosis of CAL in children with KD.

Objective To investigate the expression of histone lysine methyltransferase 2D(KMT2D) in patients with prostate cancer and its relationship with clinicopathological characteristics and prognosis. Methods A total of 126 patients with prostate cancer undergoing radical prostatectomy in the Urological Department of the Fifth People's Hospital of Datong City, Shanxi Province, from July 2015 to July 2017 were selected as the observation group, and were divided into a subgroup with good prognosis(n=88) and a subgroup with poor prognosis(n=38) based on the 3-year follow-up. In addition, 120 health examination volunteers from the same hospital were selected as the health control group. Clinical data of subjects were collected, and serum KMT2D expression levels were measured using real-time fluorescence quantitative PCR(qRT-PCR). Cox regression analysis was used to analyze factors that affect the prognosis of prostate cancer patients. The predictive value of serum KMT2D for poor prognosis of prostate cancer was analyzed using subject performance characteristic curve(ROC). Results The serum KMT2D level in the observation group was higher than that in the healthy control group(t/P=8.504/<0.001); The serum KMT2D level in the poor prognosis subgroup was higher than that in the good prognosis subgroup(t/P=6.601/<0.001); the="" relative="" expression="" of="" kmt2d="" in="" prostate="" cancer="" patients="" with="" gleason="" score="">7, microvascular invasion, TNM stage III, and lymph node metastasis was significantly higher than those with Gleason score ≤ 7, microvascular invasion, TNM stage I-II, and lymph node metastasis, respectively(t/P=3.958/<0.001, 2.292/0.024, 3.022/0.003, 5.982/<0.001); The 3-year survival rate of patients with low expression of KMT2D is significantly higher than that of patients with high expression of KMT2D(χ2/P=10.112/0.001);Cox regression analysis showed that high expression of KMT2D, Gleason score>7, TNM stage III, and lymph node metastasis were risk factors for poor prognosis in prostate cancer patients [OR(95%CI)=1.245(1.019-1.521), 1.063(1.010-1.119), 1.152(1.053-1.261), 1.474(1.127-1.928)]. ROC curve analysis showed that the area under the curve(AUC) of serum KMT2D for predicting poor prognosis in prostate cancer patients was 0.814, the sensitivity was 0.680, the specificity was 0.908, and the Jordan index was 0.588. Conclusion KMT2D is highly expressed in the serum of prostate cancer patients, which has a good predictive value for poor prognosis and is a risk factor for poor prognosis

Objective To study the expression and clinical significance of F-box leucine-rich repeat protein 2(FBXL2) and Wnt signal pathway inhibitor 1(Dkk-1) in colorectal cancer(CRC). Methods From October 2017 to May 2019, 75 patients with CRC diagnosed and treated by general surgery in Baoding First Central Hospital were selected as the study subjects. The expression levels of FBXL2, Dkk-1 mRNA and protein in CRC carcinoma and adjacent tissues were detected by fluorescence quantitative PCR and immunohistochemistry. To compare the differences of FBXL2 and Dkk-1 protein expression in cancer tissues of CRC patients with different clinicopathological characteristics. Kaplan-Meier survival analysis(Log-rank test) The relationship between the expression of FBXL2 and Dkk-1 protein and the survival and prognosis of CRC patients. Multivariate COX regression analysis was used to analyze the risk factors affecting the survival and prognosis of CRC patients. Results The expression level of FBXL2 and Dkk-1 mRNA in CRC cancer tissue was lower than that in adjacent tissues(t=25.053, 34.053,P<0.001). The positive rate of FBXL2 and Dkk-1 protein expression in CRC cancer tissue is lower than that in adjacent tissues(χ2=58.134, 51.042,P<0.001). There was a significant positive correlation between FBXL2 and Dkk-1 protein expression in CRC cancer tissue(rs=0.714,P<0.001). The positive rates of FBXL2 and Dkk-1 protein expression in cancer tissues of patients with stageⅠto stageⅡof TNM stage and negative lymph node metastasis were higher than those of patients with stage Ⅲ of TNM stage and positive lymph node metastasis. The difference was statistically significant(χ2/P=7.588/0.006, 5.220/0.022). The 3-year cumulative survival rate of patients with negative FBXL2 expression was significantly lower than that of patients with positive FBXL2 expression(χ2=5.991,P=0.014). The 3-year cumulative survival rate of DKK-1 negative expression group was significantly lower than that of positive expression group( χ2=8.058,P=0.005). Tumor stage III, positive lymph node metastasis, negative expression of FBXL2 and negative expression of Dkk-1 were independent risk factors affecting the prognosis of CRC patients [HR(95% CI)=1.613(1.223-2.126), 1.917(1.314-2.799), 1.837(1.229-2.745), 1.738(1.246-2.426),P<0.001]. Conclusion The expression of FBXL2 and Dkk-1 in CRC cancer tissue is decreased. The expression of FBXL2 and Dkk-1 is related to the tumor stage and lymph node metastasis of CRC, and is a new tumor marker to evaluate the survival and prognosis of CRC patients.

Objective To study the expression of serum microRNA(miR)-21-5p, miR-377-3p in patients with colorectal cancer(CRC) and their correlation with Wnt/β-catenin pathway and prognosis. Methods From January 2017 to January 2018, 92 patients with CRC admitted to the Oncology Department of the 989th Hospital of the Joint Service Support Force of the Chinese People's Liberation Army were selected as the CRC group, and 60 patients undergoing physical examination in the hospital during the same period were selected as the health control group. Detection of serum miR-21-5p, miR-377-3p expression and Wnt/β-Catenin pathway indicators Wnt3a β-MRNA expression levels of catenin, c-myc, and cyclin D1. Pearson correlation analysis miR-21-5p, miR-377-3p, and Wnt3a β-Correlation between expression of Catenin, c-myc, and Cyclin D1 mRNA. To compare the differences in the expression of miR-21-5p and miR-377-3p in serum of CRC patients with different clinical and pathological characteristics. Kaplan Meier curve and Logrank test were used to analyze the difference in prognosis of patients with CRC with different serum miR-21-5p and miR-377-3p expressions. Multivariate Cox regression analysis was used to analyze the prognostic factors of patients with CRC. Results Compared with the healthy control group, the expression levels of serum miR-21-5p, Wnt3a, β-catenin, c-myc, and Cyclin D1 mRNA were increased, while the expression level of miR-377-3p was decreased in the CRC group(t/P=29.202/<0.001, 52.006/<0.001, 45.973/<0.001, 39.196/<0.001, 23.119/<0.001, 38.120/<0.001). Serum miR-21-5p was positively correlated with the expression of Wnt3a, β-catenin, c-myc, and Cyclin D1 mRNA(r/P=0.404/<0.001, 0.410/0.002, 0.529/<0.001, 0.378/<0.001), while miR-377-3p was negatively correlated with the expression of Wnt3a, β-catenin, c-myc, and Cyclin D1 mRNA(r/P=-0.347/0.007,-0.408/<0.001,-0.450/<0.001,-0.419/<0.001). The serum miR-21-5p expression level in CRC patients with TNM stage III-IV and lymph node metastasis was significantly higher than that in patients with TNM stage I-II and without lymph node metastasis, while the serum miR-377-3p expression level was significantly lower(t/P=19.741/<0.001, 18.534/<0.001, 11.799/<0.001, 17.639/<0.001). The 3-year survival rate of patients with high serum miR-21-5p expression was lower than that of patients with low expression(χ2/P=17.700/<0.001), while the 3-year survival rate of patients with low miR-377-3p expression was lower than that of patients with high expression(χ2/P=21.380/<0.001). Multivariate Cox regression analysis showed that tumor TNM stage III-IV, lymph node metastasis, and elevated miR-21-5p were independent risk factors for CRC prognosis [OR(95%CI)=1.875(1.322-2.662), 1.662(1.163-2.374), 1.847(1.366-2.500)], while elevated miR-377-3p was an independent protective factor [OR(95%CI)=0.518(0.363-0.739)].Conclusion In patients with CRC, the expression of miR-21-5p in serum increases and the expression of miR-377-3p decreases, both can be regulated by Wnt/β-Catenin pathway promotes tumor progression in CRC and can be used as a tumor marker to assist in evaluating the prognosis of patients with CRC.

Objective To evaluate the diagnostic value of cervical liquid-based cytology detection p16 and its combination with high-risk human papillomavirus(HR-HPV) detection in screening cervical cancer and precancerous lesions. Methods Select 900 women who received liquid-based cytology p16, liquid-based cytology(LBP) and high-risk HPV test in the Department of Obstetrics and Gynecology of Peking University First Hospital from January 2018 to August 2019 to analyze the screening results. Positive patients were referred to colposcopy biopsy. According to the pathological results of biopsy, evaluate the recognition efficiency of different screening strategies for high-level cervical lesions. Results The positive rate of p16 expression increased with the severity of cytology and the severity of cervical lesions. P16 has the highest sensitivity and negative predictive value for identifying high-level lesions, 0.969 and 0.995 respectively. LBP has the lowest sensitivity(0.853), but the highest specificity(0.859). HR-HPV positive predictive value and accuracy rate were the lowest, 0.328 and 0.700 respectively, with statistically significant difference(P<0.05). the="" sensitivity="" of="" lbp="" and="" hpv="" combined="" screening="" modes="" was="" 0.960="" 0.992="" respectively.="" specificity="" 0.634="" 0.643="" with="" no="" significant="" p="">0.05). Conclusion p16 and p16+HPV alone can effectively identify high-level cervical lesions. p16 is convenient and easy to operate, helps to simplify the screening strategy, and has better promotion and practical clinical application value.