Objective To investigate the value of peripheral blood cold-inducible RNA binding protein(CIRP) and CXC chemokine ligand 5(CXCL5) in the diagnosis and prognosis of neonatal sepsis. Methods A total of 113 children with neonatal sepsis admitted to the Department of Pediatrics, Chengdu Xiqu Hospital from June 2021 to June 2024 were selected as the case group. According to the pediatric critical illness score, they were divided into non-critical subgroup(n = 51), critical subgroup(n = 37), and extremely critical subgroup(n = 25). Based on outcome, they were divided into good prognosis subgroup(n = 79) and poor prognosis subgroup(n = 34). According to a 1:1 ratio, 113 healthy newborns were randomly selected as the healthy control group. Serum CIRP and CXCL5 were detected by enzyme-linked immunosorbent assay. Spearman rank correlation analysis was used to analyze their correlation with disease severity. Logistic regression analysis was used to examine their correlation with poor prognosis in children with neonatal sepsis. Receiver operating characteristic(ROC) curve analysis was used to evaluate their diagnostic value and predictive efficacy for poor prognosis in neonatal sepsis. Results Compared with the healthy control group, the serum levels of CIRP and CXCL5 in the case group were increased(t/P =18.223/<0.001, 26.819/<0.001). With the aggravation of neonatal sepsis, the levels of serum CIRP and CXCL5 gradually increased(F/P = 77.582/<0.001, 177.616/<0.001). Serum CIRP and CXCL5 were positively correlated with disease severity(rs =0.682, 0.703, both P <0.001). Compared with the good prognosis subgroup, the poor prognosis subgroup had more severe disease, and the levels of serum CIRP and CXCL5 were increased(t/P = 5.504/<0.001, 8.546/<0.001). Disease severity being critical or extremely critical [compared with non-critical children, the risk of poor prognosis increased by 2.807 times and 10.859 times, respectively(P <0.05)], and elevated serum CIRP and CXCL5 were independent risk factors for poor prognosis in children with neonatal sepsis [OR(95%CI) = 1.756(1.246-2.471), 2.046(1.372-3.052)]. The AUC values of serum CIRP,CXCL5, and their combination for the diagnosis of neonatal sepsis were 0.803, 0.812, and 0.905, respectively. The AUC of the combination was greater than that of each single index(Z = 2.215, 2.079; P = 0.013, 0.009). The AUC values of serum CIRP,CXCL5, and their combination for predicting poor prognosis in neonatal sepsis were 0.764, 0.758, and 0.887, respectively. The AUC of the combination was greater than that of each single index(Z = 1.934, 1.891;P = 0.022, 0.023). Conclusion Peripheral blood CIRP and CXCL5 levels are elevated in children with neonatal sepsis and are related to disease severity and poor prognosis. Early combined detection can serve as a biomarker for auxiliary diagnosis of neonatal sepsis and prediction of poor prognosis risk.

Objective To explore the role of the AKT-EZH2-β-catenin axis in regulating the proliferation, migration,and invasion of colon cancer. Methods The expression levels of EZH2 in colon cancer tissues and SW480 colon cancer cells were analyzed using the GEPIA tool based on TCGA and GTEx databases and verified by Western blot(WB). The interaction among AKT phosphorylation, EZH2, and β-catenin was further investigated by co-immunoprecipitation(Co-IP) experiments.The effects of the AKT-EZH2-β-catenin axis on the proliferation, migration, and invasion of SW480 colon cancer cells were detected by 5-ethynyl-2 '-deoxyuridine(EdU) assay, wound healing assay, and Transwell assay. Results The results of GEPIA and WB showed that the expression of EZH2 in colon cancer tissues and SW480 colon cancer cells was significantly higher than that in normal tissues and the human colonic epithelial cell line NCM460(P <0.05). The Co-IP experimental results indicated that after AKT phosphorylation was activated by H 2O 2, the expression of p S473-AKT increased significantly(P <0.05), and both AKT and EZH2 proteins were present in the precipitation complex. The results of the Ed U assay, wound healing assay, and Transwell assay showed that after AKT phosphorylation was activated by H 2O 2, the proliferation, migration,and invasion abilities of SW480 cells were significantly enhanced(P <0.05). After silencing EZH2, the migration and invasion abilities of the cells were significantly decreased(P <0.05). When β-catenin was further silenced, this inhibitory effect became more prominent. Conclusion There is an interaction among AKT, EZH2, and β-catenin. The high expression of EZH2 may phosphorylate AKT, thereby upregulating the expression and activity of β-catenin, and ultimately promoting the proliferation,migration, and invasion of colon cancer cells.

From a historical perspective and within the context of inheritance and innovation in the theory of collateral disease, this paper systematically analyzes the academic and clinical values of the systematic research on the theory of visceral collateral disease. Although the history of research on collateral disease in traditional Chinese medicine has witnessed the development of "three milestones," a comprehensive theoretical system has never been fully established. This limitation has constrained the in-depth development of visceral syndrome differentiation in traditional Chinese medicine and the further improvement of clinical efficacy in treating refractory diseases. In the breakthrough achievement of the "fourth milestone" in contemporary research on collateral disease, the uniqueness of collateral vessel physiology, pathogenesis, and treatment is systematically studied based on the temporal and spatial differences between collateral vessels and meridians. This work systematically constructs the theoretical system of collateral disease and establishes the new discipline of collateral disease theory in traditional Chinese medicine. It provides an important theoretical foundation for guiding clinical treatment based on syndrome differentiation of visceral diseases, reveals the inherent laws governing the commonality of qi and blood circulation in collateral vessels and the unique functional structures of viscera, promotes the systematization of the theory of visceral collateral disease, and enhances the clinical efficacy in treating refractory diseases. This paper also presents phased achievements in the research on the rules of syndrome differentiation and treatment of lung collateral disease and cardiovascular continuum, aiming to initiate discussion and promote systematic research on the theory of visceral collateral disease.

This paper reports the clinical data of a patient with anti-metabolic glutamate receptor 5 encephalitis and conducts a literature review.

Department of Hepatobiliary and Pancreatic Medicine and Intervention,The Second Norman Bethune Hospital of Jilin University