To analyze the relationship between the expression level of phosphatase tensin homolog gene
( PTEN) and histone deacetylase 3 ( HDAC3) deleted on chromosome 10 and vascular cognitive impairment ( VCI) after
stroke． Methods From June 2019 to April 2021，126 stroke patients diagnosed and treated by the First Department of Neurology of Shijiazhuang People＇s Hospital were selected as the study subjects． According to whether the patients had VCI three
months after onset，they were divided into VCI group of 52 cases and non VCI group of 74 cases． Serum PTEN mＲNA was detected by real-time fluorescent quantitative PCＲ ( qＲT-PCＲ) ，and HDAC3 expression was detected by enzyme-linked immunosorbent assay; Pearson rank correlation analysis was used to analyze the correlation between the expression levels of PTEN
mＲNA and HDAC3 in serum and the scores of NIHSS and MoCA; Multivariate logistic regression analysis was used to analyze
the influencing factors of VCI after stroke．Ｒesults The serum PTEN mＲNA and HDAC3 levels in VCI group were higher than
those in non VCI group ( t /P = 17． 156 /＜ 0． 001，17． 904 /＜ 0． 001) ． Compared the expression levels of PTEN mＲNA and
HDAC3 in serum，the VCI group had severe cognitive impairment＞moderate cognitive impairment＞mild cognitive impairment
( F/P= 8．928 /＜0．001，64．433 /＜0．001) ． The NIHSS score of VCI group was higher than that of non VCI group，and the MoCA score of visual space and executive power，naming，attention，language，abstraction，delayed recall，directional score and
total score was lower than that of non VCI group，with statistical significance ( t /P= 26．197 /＜0．001，5．073 /＜0．001，13．310 /
＜0．001，11．432 /＜0．001，19．058 /＜0．001，20．211 /＜0．001，6．138 /＜0．001，3．586 /＜0．001，16．511 /＜0．001) ． Pearson correlation analysis showed that the serum PTEN mＲNA level in VCI group was significantly positively correlated with NIHSS
score，and significantly negatively correlated with MoCA score ( r = 0．713，－0．640，P＜0．001) ． The serum HDAC3 level was
significantly positively correlated with NIHSS score，and significantly negatively correlated with MoCA score ( r = 0．706，－0．
638，P＜0．001) ． Multivariate logistic regression analysis showed that hypertension，high Hcy，high hs-CＲP，high PTEN mＲNA，high HDAC3 and high NIHSS score were independent risk factors for VCI after stroke［OＲ ( 95% CI) = 2．012 ( 1．087－3．
723) ，2．587 ( 1．533－4．366) ，1．902 ( 1．063－3．404) ，1．774 ( 1．169－2．693) ，2．384 ( 1．391－4．087) ，2．895 ( 1．164－7．
202) ］．Conclusion The high expression of PTEN mＲNA and HDAC3 in serum is closely related to stroke complicated with
VCI． Both of them are risk factors for stroke patients to develop VCI

To investigate the predictive value of serum α-melanocyte-stimulating hormone ( α-MSH) and
monocyte chemoattractant protein-1 ( MCP-1) in the early assessment and prognosis of acute pancreatitis ( AP) severity．Methods From December 2019 to December 2021，117 patients with AP were diagnosed and treated in the Emergency Department
of Hefei Second People＇s Hospital /Hefei Hospital Affiliated to Anhui Medical University． They were divided into mild pancreatitis ( MAP) 45 cases，moderate There were 38 cases of severe pancreatitis ( MSAP) and 34 cases of severe pancreatitis
( SAP) ，and they were divided into a death group of 11 cases and a survival group of 106 cases according to the death situation
within 28 days． The levels of serum-α MSH and MCP-1 in AS patients with different severity and different prognosis were detected and compared，the correlation between serum α-MSH and MCP-1 and the severity was analyzed by Spearman，the influencing factors of the prognosis of AS patients，and receiver operating characteristic curve were analyzed by Logistic regression．
( ＲOC) analysis of the predictive value of serum α-MSH and MCP-1 on the prognosis of AS patients．Ｒesults The proportion
of pleural effusion and SAP on the day of admission in the death group were higher than those in the survival group ( χ2 /P= 4．
193 /0．041，2．691 /0．007) ．Serum level of α-MSH in death group was lower than survial group，and MCP-1 was higher than
survival group ( t /P= 12． 072 /＜ 0． 001，6． 390 /＜ 0． 001) ; serum α-MSH levels were compared，MAP ＞MSAP ＞SAP，while
MCP-1 levels were compared，MAP＜MSAP＜SAP ( F/P= 261．462 /＜0．001，128．199 /＜0．001) ． According to Spearman correlation analysis，serum α-MSH level was negatively correlated with AP severity ( r = －0．415，P = 0．021) ，and MCP-1 was
positively correlated with AP severity ( r = 0．520，P = 0．017) ． Logistic regression analysis showed that pleural effusion on the
day of admission，more severe disease severity，low serum α-MSH，and high MCP-1 were risk factors for the prognosis of AP
patients ［OＲ( 95%CI) = 4．768( 2．075－10．959) ，4．162 ( 1．811－9．565) ，3．747 ( 1．630－8．612) ，4．289 ( 1．866－9．857) ］．
The AUCs of serum α-MSH，MCP-1 and their combination in predicting the prognosis of AP patients were 0．843，0．880，and
0．956，and the combined effect of the two in predicting the prognosis of AP patients was better than single prediction( Z = 2．
495，1．981，P= 0．029，0．047) ．Conclusion Serum α-MSH and MCP-1 levels are closely related to the severity of AP，and
have certain predictive value for prognosis．

To explore the clinical efficacy of self-made Xiezhuo Jiedu Ｒecipe combined with mesalazine in
the treatment of patients with ulcerative colitis ( UC) ． Methods From November 2019 to November 2020，60 patients with
turbid toxin intrinsic UC were selected from Hebei Hospital of Traditional Chinese Medicine，and were divided into control
group and test group according to random number table，with 30 patients in each group． The control group was given mesalazine enteric coated tablets orally，and the test group was given self-made Xiezhuo Jiedu Ｒecipe combined treatment on the basis
of the control group． Both groups were treated for 12 weeks． The therapeutic effects of TCM syndromes of the two groups were
compared，and the changes of inflammatory indexes ( erythrocyte sedimentation rate ( ESＲ) ，C-reactive protein ( CＲP) ，procalcitonin ( PCT) ) ，Geboes histological index，Mayo activity index score and intestinal flora level before and after treatment
were compared． Ｒesults After 12 weeks of treatment，the total effective rate in the test group was 96．7%，higher than 76．7%
in the control group ( χ2 = 5．192，P= 0．023) ． After 12 weeks of treatment，the levels of ESＲ，CＲP，PCT and Geboes histological index in the two groups were lower than those before treatment ( P＜0．01) ，but there was no significant difference between
the test group and the control group ( P＞0．05) ． After 12 weeks of treatment，the Mayo activity index scores of patients in both
groups were lower than those before treatment，and those in the test group were lower than those in the control group ( t = 4．
308，P＜0．001) ． After 12 weeks of treatment，at the phyla level，the abundance of Firmicutes and Bacteroides in the two
groups increased significantly，while the abundance of Actinomyces and Proteus decreased significantly． At the genus level，
the abundance of Bifidobacterium，Clostridium pratensii，Brautzia，Bacteroides and Lactobacillus in the test group increased，
while the abundance of Shigella and Streptococcus decreased．Conclusion The self-made Xiezhuo Jiedu Ｒecipe combined with
mesalazine enteric coated tablets can improve the clinical efficacy of UC patients，inhibit inflammatory reactions，regulate the
intestinal microecology，and achieve the effect of treating UC．

To analyze the relationship between serum cystatin C ( CysC) ，monocyte chemoattractant protein 1 ( MCP-1) and the treatment effect of febuxostat in patients with hyperuricemia．Methods From December 2018 to December 2021，102 patients with hyperuricemia who were admitted to the General Medicine Department of Minhang Hospital
Affiliated to Fudan University were selected as the research subjects． The patients all received general treatment and febuxostat
treatment． The patients＇ blood uric acid ( BUA) compliance was divided into the treatment effective group of 82 cases and the
ineffective group of 20 cases． Serum CysC，MCP-1 levels and renal function indexes［serum creatinine ( SCr) ，blood urea nitrogen ( BUN) ，BUA］were detected before and after treatment． Logistic regression analysis of the relationship between serum
CysC，MCP-1 and the treatment effect of febuxostat in patients with hyperuricemia． The receiver operating characteristic curve
( ＲOC) was used to test the predictive efficacy of serum CysC and MCP-1 on the ineffectiveness of febuxostat in patients with
hyperuricemia，and a decision curve was drawn to analyze its net return． Ｒesults After 3 months of treatment，20 of 102 patients with hyperuricemia failed to reach the BUA standard，and the treatment ineffective rate was 19．61%． The duration of hyperuricemia and the proportion of patients with chronic kidney disease ( CKD) stage 3a in the ineffective group were higher
than those in the effective group ［t( χ2
) /P= 3．194 /0．002，2．723 /0．007］． Before treatment，the serum levels of SCr，BUN，
BUA，CysC and MCP-1 in the ineffective group were higher than those in the effective group ( t /P = 2．380 /0．019，2．047 /0．
043，3．838 /＜0．001，4．539 /＜0．001，5．020 /＜0．001) ． Logistic regression analysis showed that the overexpression of serum
BUA，CysC and MCP-1 were the risk factors for ineffective treatment of febuxostat in patients with hyperuricemia ［OＲ ( 95%
CI) = 1．060 ( 1．019－1．103) ，2．071 ( 1．189－3．068) ，1．055 ( 1．022－1．088) ］．ＲOC curve analysis showed that the areas under
the curve ( AUC) of serum CysC，MCP-1 and their combination in predicting the ineffectiveness of febuxostat in patients with
hyperuricemia were 0．794，0．791，and 0．803，respectively． ＲOC showed that the combined prediction of serum CysC and
MCP-1 in patients with hyperuricemia was higher than that of single index detection． Conclusion Serum CysC and MCP-1
overexpression is associated with ineffective treatment of febuxostat in patients with hyperuricemia． Combined detection of
serum CysC and MCP-1 levels can be used to predict the risk of ineffective treatment of hyperuricemia

To observe the effect of Self-made Qingre Lishi Tongluo Formula combined with etocoxib in
treating acute gouty arthritis and its influence on serum inflammatory factors． Methods From May 2020 to May 2022，96 pa￾tients with acute gouty arthritis admitted by the Department of Traditional Chinese Medicine of Enshi Clinical College of Wuhan
University were selected and divided into the control group and the research group according to the random number table，with
48 patients in each group． Both groups were given conventional treatment，the control group was given corxi tablets on the basis
of conventional treatment，and the research group was given self prepared heat clearing，dampness removing and collaterals
dredging formula on the basis of the control group． Both groups were treated for 1 week． Compare the clinical efficacy of the two
groups and the scores of clinical symptoms ( joint swelling，joint pain，joint function) ，biochemical indicators ［erythrocyte
sedimentation rate ( ESＲ) ，blood uric acid ( BUA) ］，and serum inflammatory factors ( tumor necrosis factor) before and after
treatment tumor necrosis factor-α( TNF-α) ，Interleukin ( IL) -6，IL-1 β，IL-8，IL-10，observe and record adverse reactions
during treatment． Ｒesults After one week of treatment，the total effective rate of the study group was higher than that of the
control group ( 95．83% vs．81．25%，χ2 /P= 5．031 /0．025) ．After one week of treatment，joint swelling，joint pain，joint function
score，ESＲ，BUA in both groups decreased，and the study group was lower than the control group ( t /P = 2．929 /0．004，2．
591 /0．011，2．436 /0．017，14．394 /＜0．001，11．210 /＜0．001) ． After one week of treatment，the levels of serum TNF-α，IL-6，
IL-1β，IL-8 and IL-10 in the study group were lower than those in the control group ( t /P= 9．758 /＜0．001，12．054 /＜ 0．001，
8．203 /＜ 0．001，10．532 /＜ 0．001，10．833 /＜ 0．001) ． There was no significant difference in the incidence of adverse reactions
between the two groups during treatment ( P＞0．05) ． Conclusion The combination of Self-made Qingre Lishi Tongluo Formula
and Kaoxi Tablet can reduce the related symptoms of patients with acute gouty arthritis，reduce the level of serum inflammatory
factors，with significant efficacy and safety．