To observe the effects of simvastatin on oxidative stress，insulin like growth factor-1 ( IGF-1)
and vascular endothelial growth factor ( VEGF) levels in patients with endometriosis ( EMS) after operation． Methods One
hundred and eight EMS patients diagnosed and treated in the Obstetrics and Gynecology Department of Yan＇an People＇s Hospi￾tal from December 2018 to December 2020 were selected as the research objects，and were divided into the control group and
the research group by simple randomization，with 54 patients in each group． The control group was treated with laparoscopy
combined with Goserellin acetate sustained-release implant，while the study group was treated with simvastatin on the basis of
the control group for 6 months． The curative effects of the two groups were compared，including Kupperman score，subjective
feeling score ( positive sign score，pelvic symptom score，total subjective feeling score) ，serum oxidative stress indicators
［ＲOS，vitamin E，superoxide dismutase ( SOD) ］，IGF-1，VEGF levels before and after treatment，as well as the incidence
and recurrence rate of adverse reactions． Ｒesults The complete remission rate in the study group was higher than that in the
control group ( 94．44% vs．79．63%，χ2 /P= 5．252 /0．022) ． After 6 months of treatment，Kupperman score，positive sign score，
pelvic symptom score and total subjective feeling score in the study group were lower than those in the control group ( t = 5．
683，11．073，10．155，11．797，P＜0．001) ． The levels of serum ＲOS，IGF-1 and VEGF in the study group were lower than
those in the control group，while the levels of serum VE and SOD were higher than those in the control group ( t /P= 4．009，7．
010，4．236，5．168，5．286，P＜0．001) ． There was no significant difference in the incidence of adverse reactions and recurrence
rate between the two groups ( P＞0．05) ． Conclusion Simvastatin can reduce serum IGF-1 and VEGF levels and improve oxi-
dative stress status in patients with EMS，with significant efficacy and high safety．

To explore the application value of circulating microＲNAs prediction model in the early diagnosis of preeclampsia． Methods 246 pregnant women ( gestational age ≥ 20 weeks) who underwent prenatal examination in
the Obstetrics Department of the Second Affiliated Hospital of Hainan Medical College from January 2019 to June 2021 and had
pre-eclampsia risk factors were selected as the study subjects． They were divided into eclampsia group and non-eclampsia group
according to whether they were diagnosed with pre-eclampsia during pregnancy． The content of circulating microＲNAs，soluble
vascular endothelial growth factor receptor 1 ( sFlt-1) ，placental growth factor ( PlGF) and uterine artery pulsation index ( UTPI) in pregnant women of the two groups were detected，and the clinical data of the two groups were compared． Logistic regression analysis was used to analyze the relevant factors affecting the occurrence of preeclampsia，and nomograms were used
to build a prediction model based on the content of circulating microＲNAs and verify the feasibility． The predictive efficacy of
the receiver operating characteristic curve ( ＲOC) analysis prediction model and sFlt-1/PlGF for preeclampsia． Ｒesults Preeclampsia occurred in 71 of 246 pregnant women． The family history of eclampsia，hypertension，kidney disease，proportion of
24-hour urine protein ≥ 0．3 g and mean arterial pressure ( MAP) in the eclampsia group were higher than those in the non-eclampsia group，and PLT and Alb were lower than those in the non-eclampsia group ［χ2
( U) /P= 10．894 /＜0．001，13．518 /＜0．
001，8．573 /0．003，28．612 /＜0．001，3．510 /＜0．001，4．371 /＜0．001，7．039 /＜0．001］．The levels of miＲ-16，miＲ-21，miＲ-31
and miＲ-495 in patients with eclampsia were lower than those in non-eclampsia group，while the levels of miＲ-136 and miＲ494 were higher than those in non-eclampsia group ( U/P= 3．999 /＜0．001，7．614 /＜0．001，10．239 /＜0．001，2．709 /0．007，2．
492 /0．013，3．719 /＜0．001) ． UTPI，sFlt-1，sFlt-1/PlGF levels in patients with eclampsia were significantly higher than those
in non-eclampsia patients，while PlGF levels were significantly lower than those in non-eclampsia patients ( U/P= 5．114 /＜0．
001，2．359 /0．025，5．805 /＜0．001，2．104 /0．039) ． Logistic regression analysis showed that high PLT，high Alb，high miＲ-21
and high miＲ-31 were independent protective factors affecting the occurrence of preeclampsia ［OＲ ( 95% CI) = 0．964 ( 0．938
－0．991) ，0．782 ( 0．673－0．908) ，0．384 ( 0．215－0．685) ，0．199 ( 0．097－0．409) ］，while 24h urine protein ≥ 0．3 g，high
UTPI，high sFlt-1/PlGF were independent risk factors affecting the occurrence of preeclampsia ［OＲ ( 95% CI) = 14． 821
( 3．794－24．593) ，35．601 ( 1． 291 － 981． 401) ，2． 391 ( 1． 431 － 3． 998) ］． The C-index of pre-eclampsia predicted by the
nomogram model based on the above influencing factors was 0．947 ( 95% CI 0．902－0．979) ，with good consistency and differentiation． ＲOC curve analysis showed that the predictive efficacy of nomogram model was higher than that of sFlt-1/PlGF ( Z=
6．504，P＜0．001) ，and the sensitivity and specificity were higher than that of sFlt-1/PlGF( χ2 /P = 20．218 /＜0．001，14．464 /＜
0．001) ．Conclusion The nomogram model based on miＲ-21 and miＲ-31 has a good predictive value for preeclampsia

To investigate the relationship between the levels of serum microＲNA-499a-5p ( miＲ-499a5p) and matrix metalloproteinase 16 ( MMP-16) mＲNA in children with acute respiratory distress syndrome ( AＲDS) and the severity and prognosis of the disease． Methods One hundred and five children with AＲDS admitted to the Department of Critical Medicine of Hunan Children＇s Hospital from January 2019 to January 2022 were selected as the AＲDS group． According to the oxygen index ( OI) ，they were divided into 39 mild subgroups，42 moderate subgroups，and 24 severe subgroups． According to the clinical outcome of 28 days in hospital，they were divided into 88 survival subgroups and 17 death subgroups． 47 healthy children were selected as the health control group． The serum miＲ-499a-5p and MMP-16 mＲNA levels were detected by qPCＲ． Pearson /Spearman correlation analysis was used to analyze the correlation between serum miＲ-499a-5p and MMP-16 mＲNA levels and blood gas analysis indicators in children with AＲDS． The predictive value of serum miＲ-499a-5p and MMP16 mＲNA levels on the death of children with AＲDS was analyzed by the receiver operating characteristic curve ( ＲOC) ． Ｒesults The level of serum miＲ-499a-5p in AＲDS group was lower than that in healthy control group，and the level of MMP-16 mＲNA was higher than that in healthy control group ( t = 21．349，24．932，P＜0．001) ． The level of serum miＲ-499a-5p in mild subgroup，moderate subgroup and severe subgroup decreased in turn，and the level of MMP-16 mＲNA increased in turn ( F= 215．087，99．676，P＜ 0．001) ． Serum miＲ-499a-5p level in death subgroup was lower than that in survival subgroup，and MMP-16 mＲNA level was higher than that in survival subgroup ( t = 4．074，3．907，P＜0．001) ． Pearson /Spearman correlation analysis showed that there was a negative correlation between serum miＲ-499a-5p and MMP-16 mＲNA level in children with AＲDS ( r = －0．603，P＜0．001) ． Serum miＲ-499a-5p level in children with AＲDS was negatively correlated with arterial partial pressure of carbon dioxide and OI，positively correlated with arterial partial pressure of oxygen and saturation of blood oxygen ( r = －0．662，－0．782，0．509，0．535，P＜0．001) ，and MMP-16 mＲNA level was positively correlated with arterial partial pressure of carbon dioxide and OI，negatively correlated with arterial partial pressure of oxygen and saturation of blood oxygen ( r = 0．642，0．752，－0．519，－0．587，P＜0．001) ． ＲOC curve analysis showed that the area under the curve predicted by serum miＲ499a-5p and MMP-16 mＲNA levels alone and jointly was 0．793，0．781，0．888，respectively，and the area under the curve predicted by the combination of the two was greater than that predicted alone ( Z= 1．995，2．162，P= 0．046，0．031) ． Conclusion The lower serum miＲ-499a-5p level and the higher MMP-16 mＲNA level in children with AＲDS are related to the aggravation of the disease and poor prognosis，which can be used as prognostic indicators for children with AＲDS．

To investigate the expression and clinical significance of glycosylphosphatidylinositol anchored protein 1 ( GPAA1) and Endocan mＲNA in children with acute lymphoblastic leukemia ( ALL) ． Methods Eighty-six children with ALL who were admitted to the Department of Pediatrics of the General Hospital of Ningxia Medical University from January 2018 to January 2021 were selected as the ALL group． They were divided into 70 non relapse subgroups and 16 relapse subgroups according to whether they recurred one year later． In addition，54 healthy children who were examined at the same time in the hospital were selected as the healthy control group． The expression of serum GPAA1 and Endocan mＲNA was detected by qPCＲ method． Pearson correlation analysis was used to analyze the correlation between the expression of serum GPAA1 mＲNA and Endocan mＲNA in children with ALL，multifactor logistic regression analysis was used to analyze the factors affecting the recurrence of children with ALL，and the predictive value of serum GPAA1 and Endocan mＲNA expression on the recurrence of children with ALL was analyzed by the subject＇s work characteristic curve ( ＲOC) ． Ｒesults The expression of GPAA1 and Endocan mＲNA in serum of ALL group was higher than that of healthy control group ( t = 13．375，17．485， P＜0．001) ． The expression levels of serum GPAA1 and Endocan mＲNA in the recurrent subgroup were higher than those in thenon-recurrent subgroup ( t = 4． 069，3． 791，P＜ 0． 001) ． Pearson correlation analysis showed that the expression of GPAA1 mＲNA in serum was positively correlated with that of Endocan mＲNA ( r = 0．564，P＜0．001) ． Multivariate logistic regression analysis showed that the immune phenotype T-ALL and WBC ≥ 50×109 /L，mixed lineage leukemia rearrangement，middle and high risk of risk classification，and high expression of serum GPAA1 and Endocan mＲNA were independent risk factors for relapse in children with ALL ［OＲ ( 95%CI) = 1．958 ( 1．612－4．748) ，1．465 ( 1．076－1．995) ，1．979 ( 1．094－3．581) ，2．854 ( 2．327－5．916) ，1．512 ( 1．054－2．170) ，2．133 ( 1．080－4．214) ］． ＲOC curve analysis showed that the area under the curve ( AUC) of serum GPAA1，Endocan mＲNA expression and their combined prediction of relapse in children with ALL were 0． 786，0．784 and 0．866，respectively． The AUC of the combined prediction of relapse in children with ALL was greater than that of the single prediction ( Z= 2．574，2．919，P = 0．010，0．004) ． Conclusion The up-regulated expression of serum GPAA1 and Endocan mＲNA in children with ALL is an independent risk factor of relapse in ALL children，and can be used as a predictor of relapse in ALL children．​

To investigate the effect of down-regulation of FK506-binding protein 11 ( FKBP11) on the proliferation，migration and invasion of renal cancer ( ＲCC) cells and its effect on the transforming growth factor-β1 ( TGF- β1 ) /Smad homolog 3 ( Smad3) pathway． Methods From March 2020 to March 2021，experiments were conducted in the Biological Laboratory of the People＇s Hospital of Wuhan University． The mＲNA of FKBP11 in human normal renal tubular epithelial cell line ( HK-2) and ＲCC cell line ( A498，ACHN，CaKi-1，786-O) was determined by real-time fluorescence quantitative PCＲ ( qＲT-PCＲ) and western blotting ( Western-blot) methods and protein expression levels． The 786-O cells were divided into blank group ( not transfected) ，control group ( transfected with negative control siＲNA) ，si-FKBP11 group ( transfected with si-FKBP11) and si-FKBP11 + LY364947 group ( transfected with si-FKBP11 and added TGF-β1 /Smad3 pathway inhibitor LY364947) ，the expression levels of FKBP11 mＲNA and protein in cells of each group were detected by qＲT PCＲ and Western blot，the proliferation activity of cells was detected by CCK-8 method and colony formation assay，the invasion and migration ability of cells were detected by Transwell chamber，and Western blot was detected proliferating cell nuclear antigen ( PCNA) ，E-cadherin ( E-cadherin) ，vimentin ( Vimentin) ，apoptosis inhibitory protein ( Twist) ，zinc finger transcription factor ( Snail) ，TGF-β1，Smad3 and phosphorylation in each group of cells Smad3 ( p-Smad3) protein expression levels． Ｒesults Compared with the normal renal tubular epithelial cell line HK-2，the mＲNA and protein expression levels of FKBP11 in ＲCC cell lines A498，ACHN，CaKi-1，and 786-O were significantly up-regulated ( P＜0．05) ，and the expression was the highest in 786-O cells． Compared with the control group，the expression levels of FKBP11 mＲNA and protein，the ability of cell proliferation，the ability of cell invasion and migration，and the protein levels of PCNA，Vimentin，Twist，Snail， TGF-β1 and p Smad3 in the si-FKBP11 group were significantly decreased，and the protein level of E-cadherin was significantly decreased． Compared with the si-FKBP11 group，the si-FKBP11+LY364947 group had significantly lower cell proliferation ability，cell invasion and migration ability，PCNA，Vimentin，Twist，Snail，TGF-β1 and p-Smad3 protein levels，E-cadherin protein level was significantly increased ( P＜0．05) ; however，there was no significant difference between the blank group and the control group in the changes of the above indicators ( P＞0．05) ． Conclusion Downregulation of FKBP11 can inhibit ＲCC cell proliferation，invasion and migration by inhibiting the activation of TGF-β1 /Smad3 pathway．​