Objective To investigate the effects of autoimmune function and tumor markers on recurrence after radical resection in patients with cervical cancer. Methods We conducted a study involving 108 patients diagnosed with cervical cancer who received treatment at our hospital between January 2021 and November 2022. All patients underwent radical resection for cervical cancer. Based on the occurrence of recurrence and metastasis within 1 year after treatment initiation, the patients were categorized into two groups: non-recurrent group(n=86) and recurrent group(n=22). The differences of clinical data, disease characteristics, autoimmune function and tumor markers of cervical cancer between the two groups were compared. Spearman correlation analysis and multivariate logistic regression analysis were employed to identify the risk factors for tumor recurrence in patients with cervical cancer. Furthermore, the predictive value of these risk factors in postoperative recurrence of cervical cancer was evaluated using receiver operating characteristic curve(ROC) analysis. Results In the recurring cohort, there was a notable increase in the proportion of patients displaying vascular tumor thrombus and deep muscular invasion(χ2/P=4.923/0.027, 4/284/0.038). Additionally, there was a significant decrease in the average CD4+T/CD8+T ratio and the proportion of NK cells(t/P=4.117/<0.001, 2.680/0.009, 3.708/<0.001). Conversely, the average SII level and the average CA125 and CA199 levels were markedly elevated(t/P=2.040/0.044, 2.586/0.011). Through Spearman correlation analysis and multivariate Logistic regression analysis, it was determined that a lower CD4+T/CD8+T ratio, NK cell ratio, and higher SII and CA125 levels were identified as risk factors for postoperative recurrence in cervical cancer patients [OR(95%CI)=0.019(0.010-0.048), 0.621(0.453-0.852), 1.080(1.003-1.160), 1.042(1.009-1.076)].Moreover, ROC analysis demonstrated that the CD4+T/CD8+T ratio, NK cell ratio, SII, and CA125 independently and collectively provided higher predictive value in the prognosis of postoperative recurrence in cervical cancer patients(Z/P=4.234/<0.001, 5.113/<0.001, 4.512/<0.001, 5.452/<0.001). Conclusion Patients who have undergone radical resection for cervical cancer face a daunting challenge, as their compromised immune system and elevated levels of tumor markers highlight the potential for recurrence. Proper surveillance of immune function and prompt intervention can effectively reduce the likelihood of tumor recurrence after surgery, ultimately leading to a more favorable prognosis for these patients.

Objective To investigate the effect of neoadjuvant chemotherapy(NACT) by arterial and intravenous route on chemotherapy in patients with locally advanced cervical cancer.Methods The clinical data of 178 patients with cervical cancer ⅠB2/ⅡA2(FIGO2009) who were received NACT combined with radical cervical cancer(RH) treatment and admitted to seven first-class hospitals from January 1,2009 to December 31,2016 were collected from the Beijing Obstetrics and Gynecology Hospital, Capital Medical University, Peking University People's Hospital, Peking University First Hospital, Peking University Third Hospital, Beijing Chaoyang Hospital, Beijing Anzhen Hospital and Beijing Shijitan Hospital. The patients were divided into arterial group(104 cases) and intravenous administration group(74 cases) according to different chemotherapy regimences.To compare the difference of pathological high-risk factors and clinical outcomes between the two groups, and the difference of bone marrow suppression degree after chemotherapy.Results There was no significant difference in the total effective rate between the arterial group and the intravenous administration group(P>0.05),while the lymphatic metastasis rate in arterial administration group was lower than that of the intravenous administration group(χ2/P=6.311/0.010).The hemoglobin in arterial administration group was lower than that of the intravenous administration group, and the degree of bone marrow suppression in the arterial administration group was lower than that in the intravenous administration group(t/χ2=2.864,2.299,P=0.005,0.022).The rate postoperative radiotherapy in arterial administration group was lower than that in intravenous administration group(χ2=8.029,P=0.003).Conclusion In patients with locally advanced cervical cancer, arterial chemotherapy has potential advantages in reducing postoperative pathological risk factors and the rate of postoperative supplementary radiotherapy.

Objective To study and analyze the relationship between serum long non-coding RNA KCNQ1 opposite strand/antisense transcript 1(LncRNA KCNQ1OT1), microribonucleic acid-192-5p(miR-192-5p) and short-term progression of albuminuria and prognosis in patients with diabetic nephropathy. Methods A total of 102 patients with diabetic nephropathy admitted to Department of Endocrine Rheumatology and Immunology, Qingdao Central Hospital, University of Health and Rehabilitation Sciences(Qingdao Central Hospital) from April 2021 to December 2022 were regarded as the disease group. according to the 24 h urine albumin level at time, they were separated into a non-progressing subgroup(n=78) and a progressing subgroup(n=24). According to the prognosis, they were separated into a good prognosis group(n=84) and a poor prognosis group(n=18). 58 healthy volunteers who underwent physical examination were regarded as the control group. QRT-PCR was applied to detect the relative expression levels of serum LncRNA KCNQ1OT1 and miR-192-5p. General clinical data of patients were collected and analyzed. Multivariate logistic regression was applied to analyze the influencing factors of short-term progression of albuminuria in patients. Receiver operating characteristic(ROC) curve was plotted to analyze the predictive value of serum LncRNA KCNQ1OT1, miR-192-5p on prognosis. Pearson method was applied to analyze the correlation between LncRNA KCNQ1OT1 and miR-192-5p. Results Compared with the control group, the serum LncRNA KCNQ1OT1 level in the diseased group increased, while the miR-192-5p level decreased(t/P=17.619/<0.001, 16.120/<0.001). Compared with the non-progression group, the serum LncRNA KCNQ1OT1 level in the progression group increased, while the miR-192-5p level decreased(t/P=7.698/<0.001, 9.485/<0.001). There was a statistically obvious difference in blood urea nitrogen(BUN), creatinine(Scr), fasting blood glucose(FBG), and glycated hemoglobin(HbA1c) between the two groups(χ2/P=9.835/0.002,t/P=4.582/<0.001, 3.139/0.002, 2.593/0.011, 2.356/0.020). Compared with good prognosis group, poor prognosis group showed an increase in serum LncRNA KCNQ1OT1 level and a decrease in miR-192-5p level(t/P=7.602/<0.001,9.380/<0.001). LncRNA KCNQ1OT1 is negatively correlated with miR-192-5p(r/P=-0.452/<0.001). Hypertension and elevated serum LncRNA KCNQ1OT1 levels were risk factors for short-term progression of albuminuria in patients [OR(95%CI)=1.532(1.058-2.219);1.638(1.100-2.438)], while elevated miR-192-5p level was a protective factor for short-term progression of albuminuria in patients [OR(95%CI)=0.671(0.517-0.871)]. The AUC of LncRNA KCNQ1OT1, miR-192-5p, and their combined prediction for patient prognosis was 0.808, 0.822, and 0.896, respectively. The combined prediction was obviously better than individual diagnosis of LncRNA KCNQ1OT1 and miR-192-5p(Z/P=2.030/0.042, 2.116/0.034). Conclusion The serum LncRNA KCNQ1OT1 level in patients with diabetic nephropathy is increased, and the miR-192-5p level is decreased. Both are factors influencing the short-term progression of albuminuria in patients with diabetic nephropathy, and have a certain auxiliary predictive value for the prognosis of patients.

Objective To investigate the expression of long non-coding RNA zinc finger E-box binding homeobox protein 1 antisense chain 1(LncRNA ZEB1-AS1) and long non-coding RNA SOX2 overlapping transcript(LncRNA SOX2OT) in patients with diabetic nephropathy(DN) and their correlation with renal function. Methods In this study, 106 patients with DN admitted in Department of Nephrology, Renmin Hospital of Wuhan University from November 2021 to December 2023 were collected as the DN group, and 106 patients with simple diabetes in the hospital were regarded as the control group. Both groups were collected venous blood. The serum levels of LncRNA ZEB1-AS1 and LncRNA SOX2OT were detected. According to the renal function of DN patients, they were separated into groups. The serum levels of LncRNA ZEB1-AS1 and LncRNA SOX2OT, and renal function, blood lipids, and blood glucose levels were compared. Pearson method was applied to analyze the correlation between LncRNA ZEB1-AS1 and LncRNA SOX2OT with renal function indicators. Logistic was applied to analyze the factors affecting renal function impairment in DN patients. Results The serum levels of LncRNA ZEB1-AS1 and LncRNA SOX2OT in the DN group were lower than those in the control group(t=11.471, 10.257/all P<0.001). The levels of serum LncRNA ZEB1-AS1 and LncRNA SOX2OT in the normal urine protein subgroup were higher than those in the microalbuminuria subgroup and higher than those in the large urine protein subgroup(F=58.720, 117.722, 122.493, 595.589, 53.178/all P<0.001). The levels of serum BUN,SCr, UA in the normal urine protein subgroup were lower than those in the microalbuminuria subgroup and lower than those in the large urine protein subgroup(F=122.493, 595.589, 53.178,all P<0.001). LncRNA ZEB1-AS1 was negatively correlated with BUN, SCr, UA(r=-0.487,-0.498,-0.521, P<0.001),there was a negative correlation between LncRNA SOX2OT and the above indicators(r=-0.527,-0.515,-0.534, P<0.001). Logistic model analysis showed that the course of diabetes, BUN, SCr and UA levels were risk factors for renal function damage in DN patients[OR(95%CI)=1.672(1.128-2.479), 2.839(1.534-5.253), 2.754(1.512-5.017), 2.693(1.464-4.954)], and high LncRNA ZEB1-AS1 and high LncRNA SOX2OT were protective factors [OR(95%CI)=0.875(0.798-0.959), 0.898(0.832-0.969)].Conclusion Serum levels of LncRNA ZEB1-AS1 and LncRNA SOX2OT are related to renal function in DN patients, and may be potential indicators for evaluating renal function in DN patients.

Objective To investigate the distribution of pathogenic bacteria in elderly patients with diabetes complicated with pulmonary infection, and analyze the relationship between serum levels of heat shock protein 70(HSP70) and high mobility group B1(HMGB1) with the severity and prognosis of the disease. Methods A total of 253 patients with type 2 diabetes mellitus(T2DM) combined with pulmonary infection admitted to First Affiliated Hospital of Xi 'an Jiaotong University from January 2021 to November 2023 were selected as the study objects. According to the prognosis at discharge, they were divided into good prognosis group(n=191) and poor prognosis group(n=62). The pathogenic bacteria in sputum were identified and serum HSP70 and HMGB1 levels were detected. The prognostic factors of elderly T2DM patients with pulmonary infection were analyzed using multivariate logistic regression. ROC analysis was used to predict the predictive value of serum HSP70 and HMGB1 levels for the prognosis of elderly T2DM patients with pulmonary infection. Results The proportion of age, fasting blood glucose(FBG), hypertension, chronic obstructive pulmonary disease(COPD) and severity in the poor prognosis group was significantly higher than that in the good prognosis group(t/χ2/P=6.251/<0.001, 14.949/<0.001, 4.666/0.031, 5.827/0.016, 16.530/< 0001). A total of 298 pathogenic strains were detected in 253 patient specimens, of which gram-negative bacteria accounted for 65.10%, gram-positive bacteria accounted for 28.86%, and fungi accounted for 6.04%. Serum HSP70 and HMGB1 levels in the poor prognosis group were significantly higher than those in the good prognosis group(t=11.672, 13.069, all P<0.001). Serum HSP70 and HMGB1 levels were higher in patients with severe disease than those with moderate or mild disease(F=54.146, 231.257, all P<0.001). Multivariate Logistic regression showed that high HSP70, high HMGB1, high age, high FBG, COPD and severity were the prognostic factors of elderly T2DM patients with pulmonary infection[OR(95%CI)=1.993(1.336-2.973), 1.754(1.302-2.363), 1.322(1.015-1.722), 1.876(1.401-2.512), 3.016(1.798-5.060), 3.956(2.208-7.718),all P<0.05]. The Area under the curve(AUC) of serum HSP70, HMGB1 and their combination in predicting the prognosis of elderly T2DM patients with pulmonary infection were 0.785, 0.772 and 0.897, respectively, and the combined efficacy of the two was superior to that of each alone(the difference was compared by DeLong method)(Z=3.452, 3.297, all P<0.001). Conclusion The levels of serum HSP70 and HMGB1 are closely related to the severity and prognosis of pulmonary infection in elderly T2DM patients, and their combination has high predictive value for prognosis.