Objective To analyze the effect of glycated albumin(GA) level on neonatal adverse events in pregnant women with gestational diabetes mellitus(GDM).Methods The data of 360 pregnant women with GDM who delivered at full term in the Obstetric Department of Beijing Jishuitan Hospital from January 2021 to July 2022 were retrospectively analyzed. They were divided into a group with neonatal adverse events(case group, 60 cases) and a group without neonatal adverse events(control group, 300 cases). The pregnancy data of the two groups were compared, and the regression equation was established using binary logistic regression analysis for the possible influencing factors screened by univariate analysis.Results The level of GA at the end of pregnancy was higher than that of control group(t=2.324, P=0.020), the pre-pregnancy body mass index(BMI) of the case group was higher than that of the control group(t=2.344,P=0.019), the fasting blood glucose at the end of pregnancy was higher than that of the control group(4.6±0.5 vs 4.5±0.5,t=2.518,P=0.012), and the rate of cesarean section was higher than that of the control group(χ2=5.361, P=0.021). Binary logistic regression equation was established, and GA[OR(95% CI)=1.338(1.070-1.672)]was an independent risk factor for neonatal adverse events(P=0.011).Conclusion GA level at the end of pregnancy of GDM pregnant women is correlated with the occurrence of neonatal adverse events, and the value of this index should be attached importance in clinical work.

Objective To explore the prognostic value of microRNA-132-3p(miR-132-3p) and SMAD2 in patients with Alzheimer's disease(AD). Methods From January 2020 to December 2022, 82 AD patients treated in Department of Neurology, Jiamusi Central Hospital were collected as research subjects, according to the prognosis after 6 months, they were grouped into a good prognosis group(39 cases) and a poor prognosis group(43 cases), the baseline data of the patient were recorded. Real-time fluorescence quantitative PCR(qRT-PCR) method was applied to detect serum levels of miR-132-3p, enzyme-linked immunosorbent assay was applied to detect serum levels of SMAD2; Pearson and Spearman correlations were applied to analyze and test the correlation between miR-132-3p, SMAD2 levels and baseline data in AD patients with poor prognosis; ROC curve was applied to analyze the predictive value of serum miR-132-3p and SMAD2 levels for poor prognosis in AD patients; Logistic multiple factor regression was applied to analyze the influencing factors of poor prognosis in AD patients. Results Serum levels of SMAD2 and the proportion of mid/late stage AD, CRP, and Hcy in the poor prognosis group were higher than those in the good prognosis group, while serum levels of miR-132-3p, and HDL-C were lower than those in the good prognosis group(t/χ2=7.362, 5.598, 14.021, 12.593, 7.145, 6.655, P<0.05). MiR-132-3p in AD patients with poor prognosis was negatively correlated with AD staging, CRP, Hcy, and positively correlated with HDL-C(r=-0.513,-0.492,-0.507, 0.485, P<0.001); SMAD2 in AD patients with poor prognosis was positively correlated with AD stage, CRP, Hcy, and negatively correlated with HDL-C(r=0.504, 0.527, 0.510,-0.496, P<0.001). The AUC of serum miR-132-3p and SMAD2 alone and in combination for predicting poor prognosis in AD patients was 0.828, 0.835, and 0.910, the combined predictive value of the two is greater than that of the two alone(Z=2.148, 1.964, P=0.032, 0.046). AD staging was an independent risk factor for poor prognosis in AD patients, and miR-132-3p were protective factors for poor prognosis in AD patients, SMAD2 is an independent risk factor for poor prognosis in AD patients(OR: 95%CI=2.978(1.609-5.511), P=0.001; OR: 95%CI=0.828(0.722-0.950), P=0.007; OR: 95%CI=2.826(1.776-4.497), P<0.001). Conclusion The expression of miR-132-3p is reduced in AD patients with poor prognosis, the expression of SMAD2 is increased, and they may become serum markers of poor prognosis in AD patients, which can to some extent predict the development of the disease in AD patients.

Objective To study the level of cerebrospinal fluid(CSF) secretory CD163(sCD163), α alpha 2 macroglobulin(A2M) in children with acute purulent meningitis(PM) and correlation with poor prognosis.Methods To investigate the levels of sCD163 and A2M in cerebrospinal fluid of children with acute purulent meningitis(PM) and their relationship with poor prognosis. A total of 114 children with PM who were diagnosed and treated in the Department of Pediatrics, Hainan Hospital Affiliated to Hainan Medical College/Hainan Provincial People's Hospital from January 2020 to January 2022 were enrolled as the PM group. According to the degree of central nervous system dysfunction, they were divided into severe subgroup(n=38) and general subgroup(n=76). According to the Glasgow outcome Scale at 6 months after discharge, the patients were divided into good prognosis subgroup(n=84) and poor prognosis subgroup(n=30). A total of 60 children with viral meningitis(VM) who were diagnosed and treated during the same period were enrolled as VM group, and 60 children with non-infectious diseases of the central nervous system were enrolled as control group. Enzyme-linked immunosorbent assay was used to measure the levels of sCD163 and A2M in cerebrospinal fluid. Logistic regression was used to analyze the prognostic factors of PM. Receiver operating characteristic curve was used to analyze the value of cerebrospinal fluid sCD163 and A2M in evaluating the prognosis of PM. Results The levels of sCD163 and A2M in cerebrospinal fluid of PM group were higher than those of VM group and control group(F/P=1522.470/< 0.001, 1487.801/< 0.001).The children in the severe subgroup had significantly higher levels of sCD163 and A2M in cerebrospinal fluid than those in the general subgroup(t/P=17.900/<0.001, 14.896/<0.001). The proportion of children with shock and the levels of WBC, CRP, sCD163, and A2M in cerebrospinal fluid in the poor prognosis subgroup were significantly higher than those in the good prognosis subgroup(t/P=4.878/0.027, 7.612/<0.001,16.100/<0.001,9.522/<0.001, 20.939/<0.001). Shock, cerebrospinal fluid WBC, cerebrospinal fluid CRP, cerebrospinal fluid sCD163, and cerebrospinal fluid A2M were risk factors for poor prognosis in children with PM[OR(95%CI)=1.237(1.054-1.453), 1.172(1.104-1.355), 1.202(1.208-1.406); 1.210(1.057-1.386), 1.156(1.040-1.285)]; The area under the curve(AUC) of the combination of cerebrospinal fluid CSF sCD163 and A2M in the diagnosis of PM was 0.929, which was larger than 0.880 and 0.841 predicted by CSF sCD163 or A2M alone(Z=4.572, 5.358, all P<0.001).Conclusion The levels of sCD163 and A2M in cerebrospinal fluid of children with PM are abnormally increased, and they are related to the severity of the disease. The combination of scD163 and A2M has a high predictive value for the poor prognosis of children with PM.

Objective To investigate the relationship between serum C-X-C motif chemokine ligand(CXCL) 1, CXCL16 and liver failure stage and short-term prognosis in patients with hepatitis B virus-acute-on-chronic liver failure(HBV-ACLF). Methods A total of 170 HBV-ACLF patients admitted to the Hepatology Department of Dazhou Central Hospital from January 2018 to June 2023 were included in the study group. According to the stages of liver failure, HBV-ACLF patients were divided into early subgroup(71 cases), middle subgroup(54 cases) and late subgroup(45 cases). According to the 90-day prognosis, the patients were divided into death subgroup(60 cases) and survival subgroup(110 cases), and another 100 healthy subjects were selected to be included in the control group. Serum CXCL1 and CXCL16 levels were detected by enzyme-linked immunosorbent assay. The influencing factors of death in HBV-ACLF patients were analyzed by univariate and multivariate Logistic regression. The predictive effect of serum CXCL1, CXCL16, and model of end-stage liver disease(MELD) scores in HBV-ACLF patients was evaluated by receiver operating characteristic(ROC) curves. Results Compared with the control group, the serum CXCL1 and CXCL16 levels in the study group were significantly increased(t/P=30.816, <0.001, 29.811, <0.001). Serum CXCL1 and CXCL16 levels in early subgroup, middle subgroup and late subgroup were increased successively(F/P=317.656, <0.001, 211.391, <0.001). Compared with the survival subgroup, the serum CXCL1 and CXCL16 levels in the death subgroup were significantly increased(t/P=7.775, <0.001, 7.137, <0.001). Advanced stages of liver failure [OR(95%CI)=5.069(1.322-19.441)], MELD score [OR(95%CI)=1.548(1.174-2.042)], CXCL1 [OR(95%CI)=1.914(1.162-3.153)], CXCL16 [OR(95%CI)=3.423(1.468-7.980)] elevation were independent risk factors for death in patients with HBV-ACLF. The area under the curve of serum CXCL1 and CXCL16 combined with MELD score predicted the death of HBV-ACLF patients was 0.927, which was larger than 0.786, 0.781 and 0.784 predicted by serum CXCL1, CXCL16 and MELD score alone(Z=4.594, 4.261, 4.288, all P<0.001). Conclusion The levels of serum CXCL1 and CXCL16 in HBV-ACLF patients are increase, which are relate to liver failure stage and short-term poor prognosis, the combination of serum CXCL1 and CXCL16 on the basis of MELD score has a high value in predicting the short-term prognosis of HBV-ACLF patients.

Objective To analyze the expression of miR-210 and miR-143 in serum of children with bronchial asthma and their relationship with airway inflammation. Methods A total of 186 children with bronchial asthma admitted to Department of Pediatrics Langfang City People's Hospital from January 2020 to July 2022 were selected as Bronchial asthma group, 100 healthy children who underwent physical examinations during the same period were collected as Healthy control group, qRT-PCR method was applied to detect serum levels of miR-210 and miR-143, Pearson method was applied to analyze the correlation between miR-210, miR-143 levels and TNF-α, IL-4, IL-6, EOS, FeNO, IL-10, IL-12, IgE, IFN-γ in children with bronchial asthma; Logistic regression was applied to analyze the influencing factors of bronchial asthma in children. Results The serum miR-210 level in children with bronchial asthma were obviously higher than those in healthy children, and the miR-143 level was obviously lower than those in healthy children(t=8.317, 9.545, P<0.001), the serum miR-210 level in children with mild, moderate, and severe asthma increased sequentially, while the miR-143 level decreased sequentially(F=7.566, 19.914, P<0.001). The levels of FVC, FEV 1, VE, PEF, IL-10, IL-12, IgE, IFN-γ in children with bronchial asthma were obviously lower than those in healthy children, while the levels of Raw, IL-4, IL-6, TNF-α, EOS, and FeNO were obviously higher than those in healthy children(P<0.05). The serum levels of miR-210 and miR-143 in children with bronchial asthma were related to allergies, respiratory infections, and coughing(P<0.05). Pearson analysis showed there was a negative correlation between serum miR-210 and miR-143 levels(r=-0.362, P<0.001), the serum miR-210 level was positively correlated with TNF-α, IL-4, IL-6, EOS, and FeNO(r=0.326, 0.359, 0.315, 0.334, 0.312, P<0.001), while negatively correlated with IL-10, IL-12, IgE, and IFN-γ(r=-0.406,-0.303,-0.317,-0.453, P<0.001); the level of miR-143 was negatively correlated with TNF-α, IL-4, IL-6, EOS, and FeNO(r=-0.336,-0.324,-0.323,-0.424,-0.397, P<0.001), but positively correlated with IL-10, IL-12, IgE, and IFN-γ(r=0.338, 0.338, 0.327, 0.467, P<0.001); miR-210, miR-143, TNF-α, IL-4, IL-6, EOS, FeNO, IL-10, IL-12, IgE, and IFN-γ were all influencing factors for the occurrence of bronchial asthma in children [OR(95%CI)=1.025(1.015-1.035), 0.857(0.788-0.932), 1.034(1.020-1.048), 1.136(1.059-1.219), 1.243(1.147-1.347), 1.349(1.151-1.581), 1.408(1.178-1.683), 0.932(0.900-0.965), 0.473(0.312-0.718), 0.671(0.549-0.820), 0.583(0.437-0.778)].Conclusion The in serum miR-210 level increases and the in miR-143 level decreases in children with bronchial asthma, they are closely related to the progression of airway inflammation.